Principles of enzyme action Flashcards
mechanism of action:
catalytic power of enzyme
enzymes will act as
acid/base catalyst
enzymes will
covalently bind with substrates and weaken other bonds
lock and key active site has
rigid shape
enzyme rate
no of moles produced/ second
1st order- rate depends on
[S]
rate of reaction increases as concenration
increases
higher enzyme concentration will have greater availability to ………. the reaction
catalyse
in Michaelis Menten kinetics
equilibrium: association and diss of susbtrate and enzyme is rapid equilibrium
in Michaelis Menten kinetics
steady state
ES comes to steady state and constant
as reaction is at steady state the concentration of ………… are assumed to ………… much faster than those of ……….. and ……
intermediate
equilibrate
product
susbtrate
in the linewaver burk plot what does the equation resemble
y=mx+c
