practical techniques Flashcards
1
Q
culture solid media
A
complex media
- common rich media have chemically undefined yeast
2
Q
culture media types
A
highly reproductive
rich/minimal
supplement w vitamin
3
Q
streak plating goal:
A
- reduce number of bacteria in each subsequent quadrant
4
Q
cuture-liquid media
A
seed culture
overnight growth
1% inoculum
5
Q
anaerobic —-> glycolysis —–> ……… (1) (2) ——–> ……………..
A
Fermentation
- alcoholic
- lactic acid fermentation from cytoplasm
2 ATP/glucose
6
Q
aerobic ——-> glycolysis —–>………….. (mitochondria)————-> …………………. (mitochondria) ————> …………
A
Krebs cycle
E- transport
36 ATP/glucose
7
Q
how can you reduce contamination
A
- glove box
- bunsen burner
- Luminar Flow Cabinet
8
Q
catalase test distinguishes
A
Gram pos cocci
9
Q
staph
A
pos
10
Q
strep
A
negative
11
Q
differentiation between clostrium
A
-ve
12
Q
bacillus
A
pos
13
Q
coagulase test
A
differentiate S.aureus from -ve staph
14
Q
in light microscope the transmission is
A
unpig
15
Q
the transmission in light does not allow living cells to be
A
clearly visible
