Principle 3- Part A Flashcards
_____ kills E. coli that do not invade.
Gentamycin
______does not penetrate mammalian cells.
Gentamycin
______ is used to identify certain genes that confer invasiveness on E. Coli
Genetic complementation
In order to prove that a specific gene promotes some action, is there usually a gain or loss of function mutation in genetic complementation?
Loss of function
A ____ plasmid can’t replicate in the original bacteria but can possibly replicate in another bacterium
Suicide plasma
How is the suicide plasmid containing inv loss-of-function mutation transferred from E. coli to Y.psuedotuberculosis
Sex pillus
How many recombination events are needed to replace the inv gene in Y. pseudotuberculosis with the loss-of-function inv allele?
2
_____ is a small, mobile piece of DNA
transposon
A ____ transposon is made up of a core area surrounded by an inverted repeated sequence sandwiched between direct repeat sequences flanking the IS
Simple transposon
A ____ transposon is made up of some sort of gene sandwiched between two insertion sequences
Composite transposon
Insertion of a transposon in a gene most often creates a ______ mutation
loss-of-function
A ______ marks the site of the mutation
Transposon
_____ determines which genes are virulence factors that encode exported genes
Tn-phoA mutagenesis
_____ gene encodes a periplasmic phosphatase
phoA gene
engineered phoA gene lacks ______ so expression depends on fusion to an adjacent gene after transposition
N-terminus
