DNA Technology

Question 1

Gel Electrophoresis consists of determining DNA length via

Answer 1

• Gel

- Electrical charge

Question 2

In Gel Electrophoresis, the samples are placed in

Answer 2

Wells

Question 3

What kind of charges do the wells of Gel Electrophoresis have?

Answer 3

Negative

Question 4

What determines the migration in Gel Electrophoresis?

Answer 4

Mass

Question 5

In Gel Electrophoresis, what is the marker used to locate DNA?

Answer 5

Ethidium Bromide

Question 6

Ethidium Bromide becomes fluorescent when shined with

Answer 6

UV light

Question 7

What is the function of PCR?

Answer 7

It makes copies of fragment of DNA to clone it into a plasmid and perform other experiments with it

Question 8

What are the three steps of PCR?

Answer 8

• Denaturation (96ºC)
• Primer annealing (55ºC)
• Primer Extension (72ºC)

Question 9

Reverse transcriptase takes mRNA and makes

Answer 9

cDNA to the sequence

Question 10

What is DNA Hybridization?

Answer 10

Method to compare genomic similarities between different DNA strands

Question 11

How does Southern Blot work?

Answer 11

Small fragments of DNA are placed in gel electrophoresis, with the Agarose then transferred onto a filter and marked with radio-labeled DNA

Question 12

DNA sequencing is done when

Answer 12

A sample of DNA is amplified via PCR

Question 13

What nucleotides are used in DNA sequencing?

Answer 13

ddnucleotides, which terminate elongation

Question 14

Restriction enzymes function to

Answer 14

Destroy unprotected (foreign) DNA

Question 15

Restriction enzymes are normally found in

Answer 15

Bacteria