Crossword CPA #7 Flashcards
period in which the maximum number of microbes are growing in a medium
stationary
type of media; aka synthetic media
defined
organism that prefers a pH less than 6.5
acidophiles
type of method to count cells; ie microscopic counts, serial dilution with variable plate counts
direct
how bacteria reproduce; population doubles every generation time
binary fission
organisms that prefer to grow in salty areas
halophiles
sticky formation that causes tooth decay
biofilm
organism that gets energy from chemicals
chemotroph
type of method to count cells; ie turbidity, metabolic measurements, dry weight, PCR
indirect
term used to graphically describe bacteria growth (2 terms)
exponential or logarithmic
organism that causes dental caries
Streptococcus mutans
process to preserve cells by removing all liquid in the broth so that you can deep-freeze the pellet
lyophilization
period of bacteria growth
logphase
ie a nutrient rich test tube or plate that allows bacteria to grow
medium
oxygen is deadly to these organisms
obligate anaerobes
organisms that prefer a pH greater than 7.5
alkalinophiles
ie the small amount of bacteria that you put in a nutrient rich test tube/plate to grow
inoculum
organisms that prefer to grow in very, very hot temperatures (80C+)
hyperthermophiles
how long it takes for a particular bacteria population to double
generation time
organisms that prefer a pH of 6.5 to 7.5
neutrophiles
refers to either a test tube full of microbes or the process of inoculating a test tube to allow microbes to grow
culture
ie spots of bacteria on a plate
colonies
organisms that need 2-10% of oxygen to survive (ie H. pylori)
microaerophiles
organisms that prefer to grow in cold temp. (below 15C)
psychrophiles
organisms that prefer to grow in hot, but not too hot, temps (45-80C)
thermophiles
growth-limiting nutrient for many microbes; found in amino acids
nitrogen
organisms that can survive with or without oxygen, but grow faster with O2 (ie E. coli)
facultative anaerobes
organisms that get their carbon from other organsims
heterotrophs
test tube with agar solidified at an angle to allow for more agar surface are to grow microbes
slant
period in which bacteria death exceeds new bacteria formation in a medium
deathphase
method bacteria use to estimate their population size in an area
quorum sensing
organisms that need O2 to survive
obligate aerobes
a single one of these is required for a pure culture; appears as one spot on agar plate
colony forming unit (CFU)
organisms that prefer to grow when the temperature is 20C to 40C
mesophiles
type of media; helps you pick out one particular type of bacteria if 2 different bacteria are growing on your plate due to visible differences in the media
differential
ie a broth that contains only the progeny of one bacterium cell
pure culture
organisms that can detoxify O2 to survive on O2-filled environments
aerotolerant anaerobes
would not be carbon, hydrogen, oxygen, or nitrogen
trace elements
a method in which cells of interest in a population of different cells are tagged with florescent dyes and then a machine is used to count each tagged cell
flowcytometry
type of media; allows for several different types of microbes to possibly grow
complex
chemicals needed to live; must be obtained from diet because you can’t make them in your body
growth factors
type of media; encourages your preferred bacteria to grow and not others
selective
period of slow growth after inoculation
lagphase
how cloudy your cultured media broth is
turbidity
organisms that make their own food with carbon
autotroph
organisms that get their energy from sunlight
phototroph
liquid media
broth
