Crossword CPA #13

Question 1

restriction enzymes

Answer 1

DNA scissors

Question 2

electroporation

Answer 2

scientific procedure that uses electricity to insert foreign DNA into bacteria cell

Question 3

vector

Answer 3

used by scientists to insert small sequences of DNA into an organism for easy DNA expression

Question 4

northern blot

Answer 4

a method to confirm the presence but not location in a tissue sample of RNA sequences of interest in a sample using a probe

Question 5

probes

Answer 5

synthetic sequences of nucleotides that visually identify the location of a DNA/RNA sequence; can be used on a microscope slide on a tissue sample; northern or southern blot

Question 6

invivo

Answer 6

something that occurs inside a living organism

Question 7

southern blot

Answer 7

method to confirm the presence of DNA sequences of interest in sample using a probe

Question 8

transgenic organisms

Answer 8

plants or animals that have been genetically altered

Question 9

blunt end

Answer 9

type of DNA cut by restriction enzymes in which all hydrogen are still satisfied between nucleotides

Question 10

sticky end

Answer 10

type of DNA cut by restriction enzymes that leaves nucleotides with unsatisfied hydrogen bonds between nucleotides

Question 11

invitro

Answer 11

something that occurs outside a living organism

Question 12

DNA microarray

Answer 12

method used to monitor gene expression of dozens of genes at the same time

Question 12

gel electrophoresis

Answer 12

scientific procedure that allows you to separate molecules (DNA, RNA, proteins), based on shape, charge, and size

Question 13

gene library

Answer 13

contains all the genes of interest for scientists to use in experiments

Question 14

microinjection

Answer 14

scientific procedure of inserting DNA directly into a cell using a very fine micropipette

Question 14

cDNA

Answer 14

type of DNA made from mRNA that does not occur naturally in huhmans

Question 15

xenotransplantation

Answer 15

when animal cells/tissues/organs are placed into the human body for use

Question 16

reverse transcriptase

Answer 16

unusual enzyme that makes DNA from RNA

Question 17

genetic mapping

Answer 17

a method for locating genes and their arrangement together in DNA

Question 18

nucleotide sequencing

Answer 18

a method that identifies all the nucleotides and their order in a particular DNA segment

Question 19

protoplast fusion

Answer 19

scientific procedure in which chemicals that digest cell walls are used to merge two cells together

Question 20

fluorescentinsituhybridization

Answer 20

scientific procedure that allows scientists to see where a particular mRNA is expressed using probe; can be used on a microscope slide on a tissue sample

Question 21

mutagens

Answer 21

agents that can cause unintentional changes in the sequence of a genome

Question 22

western blot

Answer 22

method to confirm the presence of proteins of interest in a sample using a “tagged” antibody

Question 23

PCR

Answer 23

method used to create several exact copies of a short DNA segment

Question 24

recombination

Answer 24

manipulation of DNA via scientific techniques