Crossword CPA #13 Flashcards
restriction enzymes
DNA scissors
electroporation
scientific procedure that uses electricity to insert foreign DNA into bacteria cell
vector
used by scientists to insert small sequences of DNA into an organism for easy DNA expression
northern blot
a method to confirm the presence but not location in a tissue sample of RNA sequences of interest in a sample using a probe
probes
synthetic sequences of nucleotides that visually identify the location of a DNA/RNA sequence; can be used on a microscope slide on a tissue sample; northern or southern blot
invivo
something that occurs inside a living organism
southern blot
method to confirm the presence of DNA sequences of interest in sample using a probe
transgenic organisms
plants or animals that have been genetically altered
blunt end
type of DNA cut by restriction enzymes in which all hydrogen are still satisfied between nucleotides
sticky end
type of DNA cut by restriction enzymes that leaves nucleotides with unsatisfied hydrogen bonds between nucleotides
invitro
something that occurs outside a living organism
DNA microarray
method used to monitor gene expression of dozens of genes at the same time
gel electrophoresis
scientific procedure that allows you to separate molecules (DNA, RNA, proteins), based on shape, charge, and size
gene library
contains all the genes of interest for scientists to use in experiments
microinjection
scientific procedure of inserting DNA directly into a cell using a very fine micropipette
cDNA
type of DNA made from mRNA that does not occur naturally in huhmans
xenotransplantation
when animal cells/tissues/organs are placed into the human body for use
reverse transcriptase
unusual enzyme that makes DNA from RNA
genetic mapping
a method for locating genes and their arrangement together in DNA
nucleotide sequencing
a method that identifies all the nucleotides and their order in a particular DNA segment
protoplast fusion
scientific procedure in which chemicals that digest cell walls are used to merge two cells together
fluorescentinsituhybridization
scientific procedure that allows scientists to see where a particular mRNA is expressed using probe; can be used on a microscope slide on a tissue sample
mutagens
agents that can cause unintentional changes in the sequence of a genome
western blot
method to confirm the presence of proteins of interest in a sample using a “tagged” antibody
PCR
method used to create several exact copies of a short DNA segment
recombination
manipulation of DNA via scientific techniques
