Acid-Fast Staining (Kinyoun Method) Flashcards
1
Q
primary dye
A
carbolfuchsin
2
Q
decolorizer
A
37% HCL & alcohol
3
Q
counter stain
A
methylene blue
4
Q
acid-fast negative cells
A
blue; NO mycolic acid
5
Q
acid-fast positive cells
A
red-purple; YES mycolic acid
6
Q
what is mycolic acid?
A
waxy coating
7
Q
mycolic acid is found in what cells?
A
acid-fast positive
8
Q
forgetting to use decolorizer causes what?
A
false positive; acid-fast negative cells will be stained red-purple and appear to be acid-fast positive
9
Q
too long of exposure to decolorizer will cause what?
A
false negative; acid-fast positive cells will be stained blue and appear to be acid-fast negative
10
Q
common acid-fast positive organisms have names that start with what?
A
mycobacterium (ex. TB & leprosy); nocardia
