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Bacteriology > Unit 4- Practical Diagnostics > Flashcards

Unit 4- Practical Diagnostics Flashcards

1
Q

Abscess Collection

A

Scrape wall with pus

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2
Q

Exudate Collection

A

Aseptically with sterile syringe

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3
Q

Swab Collection

A

Use culturette transport system

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4
Q

Sample Preservation

A

Must be kept cold, feces should be in transport media at room temp, aseptic technique essential, bone marrow or rib from animals that have been dead for a long time

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5
Q

Anaerobic Culture

A

Anaerobic samples with normal flora cannot be cultured because they are contaminated with anaerobic normal flora

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6
Q

Anaerobic Shipment

A

Anaerobic transport system if not reaching lab within 30 min, large tissue will maintain anaerobic environment but must be refridgerated

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7
Q

Mastitis Procedures

A

Culture first few mLs of milk expressed and collect sample from within udder, collect into sterile narrow-necked bottles

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8
Q

UTI Procedures

A

Specimens must be refrigerated and cultured within 6 hours, innoculate to blood or MacConkey with sterile loop, most commonly enterics

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9
Q

Abortion Procedures

A

Must be comprehensive for all bacteria and fungi, maternal and fetal samples, fresh and formalin-fixed for histology

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10
Q

Fetus Samples

A

Placenta and cotyledons, uterine discharge, fetal stomach contents, fetal lung, liver, kidney, and spleen

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11
Q

Leptospirosis Abortion Samples

A

Midstream urine sample must be centrifuged and darkfield microscopied immediately, clotted blood samples at the time of abortion and 2 weeks later

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12
Q

Infertility Specimens

A

Uterine discharge, vaginal mucus, preputial washings, semen, urine

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13
Q

Cattle Infertility Samples

A

Should include Campylobacter fetus and opportunistic aerobes, culture within 4-6 hours, special transport medium for C. fetus

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14
Q

Mare Infertility Samples

A

Coliforms and other opportunistic aerobes

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15
Q

Blood Cultures

A

Skin shaved and aseptically prepped, 3 blood samples taken 1 hour apart

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16
Q

Johne’s Samples

A

Scraping of rectal mucosa in live animal or ileocecal valve in dead animal, can be examined by acid fast stain or PCR

17
Q

Diarrhea Samples

A

Campylobacter, Salmonella, and Yersinia, possible toxins in foods, E coli in neonates, fecal samples in live animal or mesenteric lymph nodes, liver, and intestinal contents in dead

18
Q

Swine Intestinal Disease Samples

A

Recently dead or killed pig, hemorrhagic intestine, or mucosal scrapings

19
Q

Swine Dysentery Samples

A

Brachyspira diagnosed by microscopy, feces from live animal or intestine and contents from dead

20
Q

Proliferative Enteropathy Samples

A

Lawsonia intracellularis, cannot be cultured, diagnose by PCR of feces or histopathology

21
Q

Tuberculosis Samples

A

Acid fast staining of smears, lesions sampled

22
Q

Mycoplasma Samples

A

Cultivate on mycoplasma media in CO2, differentiate with antisera

23
Q

Chlamydia Samples

A

Not culturable on culture media, submit to virology lab for culture on tissue culture, serology or ELISA

24
Q

Anthrax Samples

A

State or federal reference lab

25
Q

Systemic Fungal Infection Samples

A

Lesions for culture and histopathology

26
Q

Dermatophyte Infection Samples

A

Plucked hairs, skin scrapings, and scalpel in paper envelope, innoculated onto dermatophyte test medium, Wood’s lamp

27
Q

Streptothricosis or Dermatophiliasis Samples

A

Scabs with hairs stained by methylene blue

28
Q

Fluorescent Antibody Technique

A

Diagnose malignant edema and blackleg from rib bone marrow or muscle, Chlamydia, Leptospira, and Brucella from direct tissue

29
Q

Latex Agglutination

A

Detect antigen or antibody, rapid results

30
Q

PCR

A

Amplifies DNA, real time PCR must be used for diagnosis with the cycle threshold for diagnosis

31
Q

Gram + Cocci

A

Staphylococcus and Streptococcus

32
Q

Gram - Cocci

A

Erysipelothrix, Listeria, Cornyebacterium, Trueperella pyogenes

33
Q

Gram - Lactose Fermenters

A

E. coli, Klebsiella, Enterobacter

34
Q

Gram- Glucose Fermenters

A

Proteus, Salmonella, Citrobacter

35
Q

Oxidase Fermentors

A

Pasteruella, Actinobacillus, Pasteurella multilocida, Manheimmia haemolytica, Hemophilus

36
Q

Gram- GLucose Oxidizers

A

Psuedomonas aeruginosa

Bacteriology (44 decks)

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