Unit 3- Francisella

Question 1

Francisella tularensis

Answer 1

Category A select agent requiring BSL-3, gram neg coccobacillus, aerobe, bipolar stain with giemsa, cysteine required for growth, no growth on blood agar, slow growth

Question 2

Tularemia

Answer 2

Transmitted by arthropod, water, infected carcass, common in wild animals and rabbits, cats and sheep infected, lesions in liver, spleen, and lungs, intracellular

Question 3

Human Tularemia

Answer 3

Skin ulcer and lymph node swelling, 7 forms based on route of infection, nausea, diarrhea, vomiting, sweats, fever, weakness

Question 4

Francisella tularensis subsp tularensis

Answer 4

Type A, tier 1 bioterrorism agent, only in north america and associated with land

Question 5

Francisella tularensis subsp holarctica

Answer 5

Type B, less virulent than type A, worldwide and associated with water

Question 6

Francisella Transmission

Answer 6

Wildlife and arthropod vector, systemic disease and granulomatous lesions, long environmental survival, human zoonosis

Question 7

Francisella Virulence Properties

Answer 7

Capsule, LPS, intracellular invasion, pathogenicity island, invisible pathogen

Question 8

Francisella Compromising Factors

Answer 8

Frank pathogen, highly virulent with low infectious dose

Question 9

Francisella Immunity

Answer 9

Cellular immunity required, antibodies cannot reach intracellular, T cells kill infected cells

Question 10

Francisella Diagnosis

Answer 10

Cysteine-glucose-blood-agar, slow growing, BSL-3 lab, serology, FA, PCR