Unit 1- Taxonomy Flashcards
Frank Pathogens
Cause disease in any host, considered a pathogen when isolated
Opportunistic Pathogens
Capable of causing disease given the opportunity, often normal part of flora, capable of causing disease in normally sterile tissues
Non-Pathogens
Can cause disease in hosts with weak immune system
D=N*V/R
Disease equals number of organisms times virulence divided by resistance
Bacterial Classification Schemes
Speed up identification using a limited number of characteristics
Bacterial Nomenclature
Binomial system with specific naming conventions that assigns a genus and species
Bacterial Identification
Based on morphological, biochemical, and serological traits
Gram-Positive
Thick peptidoglycan wall and appear blue/violet after staining
Gram-Negative
Thin peptidoglycan wall but lipid-rich outer membrane and appear red after staining
Gram Stain Procedure
Make a thin smear, stain with crystal violet, stain with Gram’s iodine, stain with safranin, rinse
Peptidoglycan Network
Lyseine and alanine are linked
β-Lactams
Bind penicillin-binding proteins and inhibit peptidoglycan synthesis
Lysozyme
Breaks bonds between tetrapeptides in peptidoglycan network
Disruption of Peptidoglycan
Lysozyme or penicillin will cause cytoplasmic membrane blebbing, and fragmentation
Acid Fast Bacteria
Mycolic acids connect muramic acid and lipids and prevent acid decolorization, staining red
Lipopolysaccharide
Endotoxin in gram-negative bacterial outer membrane, promotes secretion of pro-inflammatory cytokines in macrophages
Bacteremia
Bacteria in blood
Endotoxic Shock
Continuing host response to endotoxin and inflammation cause disease
Flagella
Filamentous appendages that allow for motility
Glycocalyx
Capsule with a lipid anchor that is associated with the cell
H Antigens
Flagella
K antigens
Capsule antigens
Exopolysaccharide
Slime layer surrounds capsule and forms biofilm
Pili
Filamentous appendages that allow for adhesion and sex pili, highly antigenic and can be used for diagnosis
Fimbriae
Pili used for adhesion
Sex Pili
Allow transfer of DNA between donor and recipient
Fermentation
Anaerobic glycolysis, produces acids and alcohols
Respiration
Aerobic oxidation
Photosynthesis
Light absorbed by pigments to generate ATP
Thermophiles
Prefer hot temperatures
Mesophiles
Prefer warm temperatures
Psychrophiles
Prefer cool temperatures
Facultative Anaerobe
Grows with or without oxygen
Microaerophile
Requires reduced oxygen
Sugar breakdown
Aerobic oxidation or anaerobic fermentation
Capnophile
Requires increased carbon dioxide
Basic Media Types
Defined/simple or enriched/complex
Identification Media
Differential, selective, enriched, transport
MacConkey Agar
Differential media that changes color if the bacteria ferments lactose
Stewart’s Transport Medium
Used for transport of samples, helps scavenge oxygen to preserve anaerobes, semi-solid consistency to minimize oxygenation
Metabolism
Net result of biochemical activities associated with catabolism and anabolism, leading to growth
ATP
Generation synonymous with energy generation
Metabolic Footprint
Byproducts associated with metabolism can be used to ID bacteria