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Bacteriology > Unit 2- Salmonella > Flashcards

Unit 2- Salmonella Flashcards

1
Q

Enterobacteriaceae

A

Gram negative, rod shaped, catalase positive, oxidase negative, facultative anaerobe, non fastidious, non spore forming, grow on bile, most motile

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2
Q

Ceek Group

A

Lactose fermenters; Citrobacter, E. coli, Enterobacter, Klebsiella

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3
Q

SPY-Sh Group

A

Lactose non fermenters, highly pathogenic; Salmonella, Proteus, Yersinia, Shigella

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4
Q

Enterobacteriaceae Diagnostics

A

Lactose test for CEEK vs SPY-Sh, gas production, motility, IMVic test

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5
Q

Enterobacter Tests

A

Lactose fermenter, fast growth, CO2 producer, urease negative

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6
Q

Klebsiella Tests

A

Lactose fermenter, fast growth, CO2 producer, urease positive

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7
Q

Citrobacter Tests

A

Lactose fermenter, slow growth, H2S producer, urease positive

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8
Q

Proteus Tests

A

Non lactose fermenter, H2S producer on TSI, urease positive

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9
Q

Salmonella Tests

A

Non lactose fermenter, H2S producer on TSI, urease negative

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10
Q

Yersinia Tests

A

Non lactose fermenter, no gas production, urease positive

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11
Q

Urease Negative Enterobacteria

A

Salmonella and enterobacter

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12
Q

H2S Gas on TSI Enterobacteria

A

Salmonella, proteus, citrobacter

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13
Q

Enterobacteria Habitat

A

Small and large intestine

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14
Q

Enterobacteria Transmission

A

Oral-fecal transmission, commensal of digestive but can cause disease

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15
Q

Enterobacteriaceae Disease

A

GI infection, diarrhea, liver abscess, UTI, bacteremia, meningitis, mastitis

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16
Q

Enterobacteriaceae Surface Structures

A

Capsule, flagella, fimbriae, adhesins, invesins, LPS

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17
Q

Enterobacteriaceae Enzymes

A

Catalase and superoxide dismutase

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18
Q

Enterobacteriaceae Toxins

A

Hemolysin, siderophores, pathogenicity island, type 3 secretion system

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19
Q

Pathogenicity Island

A

Area of genome encoding toxins, enzymes, and type 3 secretion system

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20
Q

Type 3 Secretion System

A

Syringe apparatus on the cell wall to inject enzymes, toxins, and cytokine inhibitors

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21
Q

Enterobacteriaceae Antigenicity

A

Wide diversity due to LPS, flagella, and capsular antigen

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22
Q

Enterobacteriaceae Culture

A

Blood agar, Mac Conkey agar, eosin methylene blue, TSI

23
Q

AMR Genera

A

Cabapenem resistance from Klebsiella, Enterobacter and E. coli

24
Q

Salmonella Gas Production

A

H2S, rotten egg smell and black colonies

25
Q

Salmonella Culture

A

MacConkey, eosin methylene blue, brilliant green, samonella and shigella agar

26
Q

Selective Salmonella Media

A

Brilliant green or salmonella and shigella agar, perform TSI test, serotype

27
Q

Salmonella Habitat

A

Normal digestive flora, S. typhimurium on reptile and amphibian skin

28
Q

Transmission

A

Oral fecal, transovarian in poultry

29
Q

Salmonella Serotypes

A

2500 based on combinations of LPS and flagella antigen

30
Q

Salmonella Species

A

S. enterica and S. bongori

31
Q

Salmonella enterica Subspecies

A

enterica I, salmae II, arizonae IIIa, diarizonae IIIb, houtanae IV, indica VI

32
Q

Salmonella Serotype Grouping

A

Host specific, host adapted, broad host range

33
Q

Salmonella zoonosis

A

All S enterica are zoonotic besides S. pullorum and gallinarium

34
Q

Major Human Salmonella Serotypes

A

S typhurium, newport, and enteridis

35
Q

Salmonella in Poultry

A

S pullorum, gallinarium, and enterica var enteridis among 7 priority diseases for poultry improvement plan

36
Q

Salmonella in Songbirds

A

Birds carry zoonotic salmonella

37
Q

Salmonella Pathogenicity Island

A

SPI 1 and 2 present on virulence plasmid or salmonella genome, encoding type 3 secretion systems

38
Q

Salmonella Body Structures

A

Fimbriae, flagella (besides chicken species), capsule, LPS lipid A causing ischemic necrosis

39
Q

Salmonella Enzymes

A

Catalase, superoxide dismutase, siderophores

40
Q

Salmonella Toxins

A

Type 3 secretion systems, adhesins, invasins, hemagglutinins, exotoxins, siderophores

41
Q

Salmonella Infection

A

Attaches to cell, T3SS-1 injects for intracellular invasion, T3SS-2 injects for intracellular multiplication, lyses cell to escape

42
Q

Genes for Intracellular Invasion

A

T3SS, effector proteins for invasion and penetration, encoded on plasmid or genome of SPI

43
Q

Salmonella Systemic Invasion

A

Incubation period in lymphoid tissue, enters blood stream after stress, can result in severe complications

44
Q

Silent Salmonellitis

A

Infected animals harbor in a chronic carrier state, stress causes flare ups

45
Q

Typhoid Salmonellitis

A

Pneumonia, myocarditis, meningioencephalitis, septicemia, dry gangrene, mastitis, abortion, arthritis

46
Q

Enteric Salmonellitis

A

GI upset, fever, weakness, shock, discharge, abortion, colic, diarrhea

47
Q

Young Chicken Salmonellitis

A

S. pullorum causes viscous white diarrhea

48
Q

Old Chicken Salmonellitis

A

S. gallinarum causes watery yellow diarrhea

49
Q

Salmonella Diagnosis

A

Gram - rod, oxidase -, lactose - on macconkey, H2S on TSI, motile, urease -, selective brilliant green agar and salmonella and shigella agar

50
Q

Salmonella Serotyping

A

Done by LPS (O) and flagella (H) antigens

51
Q

Salmonella Treatment

A

Low cure rate with antimicrobials, electrolyte and fluid therapy

52
Q

Salmonella Control

A

Hygiene, water disinfection, iodine

53
Q

Salmonella Vaccine

A

Poultry specific serovars and S enteritidis

54
Q

Shoat Abortion

A

S. abortusovis

Bacteriology (44 decks)

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