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Bacteriology > Unit 3- Brucella > Flashcards

Unit 3- Brucella Flashcards

1
Q

Brucella

A

Coccobacillus, single cells, gram neg, aerobes, catalase oxidase and urea +, non motile, intracellular, bioterrorism agent

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2
Q

Brucella Nomenspecies Differentiation

A

Biochemical reactivity, biotyping by aniline dyes, antigen composition, bacteriophage susceptibility, DNA specificity

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3
Q

Horse Brucella Species

A

B. abortus

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4
Q

Pig Brucella Species

A

B suis

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5
Q

Dog Brucella Species

A

B canis

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6
Q

Cattle Brucella Species

A

B abortus and suis

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7
Q

Sheep Brucella Species

A

B melitensis and ovis

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8
Q

Goat Brucella Species

A

B melitensis

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9
Q

Brucella Intracellular Activity

A

Facultative intracellular pathogen, inhibits phagosome-lysosome fusion, replicates in macrophages

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10
Q

Smooth Nomenspecies

A

B abortus, melitensis, neotomae, and suis, O antigen involved in agglutination

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11
Q

Rough Nomenspecies

A

B canis and ovis, rough LPS lacks O antigen

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12
Q

Brucellosis Transmission

A

Carrier animals, ingestion most common, veneral or congenital

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13
Q

Zoonotic Species

A

B abortus, melitensis, suis, and canis

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14
Q

Brucella abortus

A

Ingestion, venereal, intra-mammary and congenital transmission, some cattle resistant to infection

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15
Q

B abortus Pathogenesis

A

Penetrates mucous membranes, localize in lymph nodes, phagocytosis by neutrophils and macrophages, bacteremia, proliferates in cells with erythritol

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16
Q

B abortus Disease

A

Abortion at end of first pregnancy, placental thickening, necrosis of cotyledons, edematous fetus

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17
Q

B abortus Diagnosis

A

Notifiable zoonotic disease, culture sent to BSL-3 facility, PCR, serology for LPS, rivanol or mercaptoethanol test, compliment fixation

18
Q

Rivanol Test

A

Distinguishes between chronic infection or vaccination, IgG higher than IgM in infection

19
Q

B abortus Immunology

A

Humoral antibody response not correlated with protection, cell mediated immunity from T cells most important, causes granulomatous lesions

20
Q

B abortus Vaccination

A

RB51 most protection and interrupts outbreaks, 19 less protection and can cause abortion, McEwen 45/20 requires boosters

21
Q

B abortus Control

A

Test and slaughter, vaccinate, hygiene, Ab milk ring test with high false +

22
Q

B abortus Therapy

A

Not attempted in pig and ruminant, cull infected animals, long term therapy for intracellular infection

23
Q

B melitensis

A

First Brucella isolated, bio-warfare agent, very low infectious dose in human

24
Q

B melitensis Transmission

A

Ingestion, food and water contaminated by urine and vaginal discharge, milk and cheese

25
Q

B melitensis Disease

A

Pathogenesis similar to B abortus, late term abortion in sheep and goat, lameness, hygroma, orchitis

26
Q

B melitensis Immunology

A

Sheep and goats develop protective immune response and clear infection

27
Q

B melitensis Diagnosis

A

Clinical signs, direct culture, serologic tests

28
Q

B melitensis Vaccination

A

Strain Rev 1 most effective and reduces shedding, strain 53H38 stimulates persistent antibody response

29
Q

Brucella suis Transmission

A

Rapid spread, venereal, ingestion, wild hare reservoir, environmental persistence

30
Q

B suis Disease

A

Pathogenesis similar to B abortus, more common in adult swine, abortion, metritis, spondylitis, orchitis, arthritis, lameness, bacteremia

31
Q

Most likely pathogen to humans

A

B suis, cannot be eradicated due to feral swine

32
Q

B suis Immunology

A

Females recover, males do not, short lived immunity, ready reinfection, abortion only in first pregnancy

33
Q

B suis Diagnosis

A

Rose bengal card test, clinical signs, culture

34
Q

Brucella canis Transmission

A

Ingestion, veneral

35
Q

B canis Disease

A

Pathogenesis similar to B abortus, intermittent persistent bacteremia, late term abortion, lymphadenitis, infertility, sick puppy, epididymitis, scrotal dermatitis, unilateral scrotal atrophy

36
Q

B canis Immunology

A

Humoral response during bacteremia, cell mediated protective immunity

37
Q

B canis Diagnosis

A

Clinical signs, direct culture, immunodiffusion

38
Q

Serologic Tests

A

Do not work for rough variants lacking O antigen, false positives

39
Q

B canis Control

A

Euthanasia

40
Q

Brucella ovis Transmission

41
Q

B ovis Disease

A

Pathogenesis similar to B abortus, sheeps, epididymis, late abortion, low fertility, rams affected more frequently

42
Q

Brucella Zoonosis

A

Highly infectious, melitensis most pathogenic

Bacteriology (44 decks)

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