Unit 1- Staphylococcus Flashcards

1
Q

Genus Staphylococcus

A

gram positive cocci in clusters, facultative anaerobe, non-motile, non-spore forming, catalase positive, grow on high salt media

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2
Q

Cultures for growth

A

Mannitol salt agar and blood agar

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3
Q

Mannitol Salt Agar

A

Selectively supports the growth of gram positive staphylococcus, enterococcus, listeria, and micrococcaceae and inhibits streptococcus and gram negatives

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4
Q

S. Aureus on Mannitol salt

A

Ferments Mannitol and turns agar from red to yellow

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5
Q

S. epidermidis on Mannitol salt

A

Does not ferment and agar remains the same

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6
Q

S. aureus on purple agar base

A

Turns agar yello

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7
Q

Beta hemolysis

A

Complete hemolysis, dangerous species, turns blood agar clear

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8
Q

Alpha hemolysis

A

Incomplete hemolysis, turns agar green

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9
Q

Gamma hemolysis

A

No hemolysis or change in media

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10
Q

Staphyloxanthin

A

Carotenoid pigment in S. aureus indicating more pathogenicity

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11
Q

Catalase

A

Test for antioxidant, Staphylococcus will be positive

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12
Q

Coagulase

A

Causes serum to clot, positive strains are more pathogenic

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13
Q

Staph Habitat

A

Commensal in skin and tubular orifices

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14
Q

Risk of Staph Infection

A

Other infection, shearing in sheep, haircut in dogs, or nutritional imbalance

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15
Q

Body structures for adhesion

A

Cell wall, protein A, binding factors, and capsule or slime layer

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16
Q

Protein A

A

Binds the arms of IgG antibodies, blocks immune response and opsonization

17
Q

Enzymes for invasion

A

Hyaluronidase, catalase, coagulase, staphylokinase, lipase, protease, beta-lactamases

18
Q

Staphylokinase

A

Lyses fibrin in blood clots to disperse itself

19
Q

Toxins

A

Hemolysin, cytolytic toxins, exfoliative toxins, toxic shock syndrome toxin, enterotoxins

20
Q

Toxic Shock Syndrome Toxin

A

Causes shock and superantigens activating 20% of T cells

21
Q

Pathogenesis

A

Adhesion on host cell, biofilm formation, intracellular invasion, inflammation and abscess formation, and invasion of blood and systemic spread

22
Q

Abscess Rupture

A

Leads to bacteremia and diverse organ infection in organs that receive higher cardiac output

23
Q

Coagulase Positive Species

A

S. aureus, S. hyicus, S. pseudointermedius, S. shleiferi

24
Q

Coagulase Negative Species

A

S. chromogenes, S. felis, S. epidermidis

25
Q

Staphylococcus aureus

A

Causes pus in abscess, dermatitis, mastitis, septicemia, endocarditis, meningitis, and osteoarthritis in many species

26
Q

Staphylococcus pseudointermedius

A

Most common in pets, causes pyoderma, pyometra, and otitis externa

27
Q

Staphylococcus hyicus

A

Most common in pigs, causes greasy pig disease

28
Q

Scalded Skin Disease

A

S. aureus causes exudative epidermitis in humans

29
Q

Greasy Pig Disease

A

S. hyicus causes exudative epidermitis in pigs

30
Q

Pyoderma

A

S. aureus and S. schleiferi causes pyoderma of dogs and cats

31
Q

Otitis externa

A

S. pseudointermedius and S. aureus causes otitis externa in dogs and cats

32
Q

Mastitis

A

S. aureus and S. chromogenes are commensal at teat orifices and can cause mastitis in ruminants

33
Q

Largest cause of mastitis

A

Staphylococcus

34
Q

Tick Pyemia

A

S. aureus causes pus-forming bacteria to spread in the blood stream after abscess caused by tick bites ruptures

35
Q

Botryomycosis

A

Cluster of grape bumps on skin of horses caused by S. aureus

36
Q

Bumble Foot

A

Pyogranulomatous infection in the foot of poultry caused by S. aureus

37
Q

Resistance

A

Methicillin and vancomycin

38
Q

Control and prevention

A

Improve hygiene and wellbeing: improve immunity, tick control, clean wounds, carefully groom

39
Q

Control and prevention in dairy

A

Hygiene at milking time, dry cows, separate infected cows, cull cows with chronic mastitis, prevent reinfection