Unit 1- Staphylococcus Flashcards
Genus Staphylococcus
gram positive cocci in clusters, facultative anaerobe, non-motile, non-spore forming, catalase positive, grow on high salt media
Cultures for growth
Mannitol salt agar and blood agar
Mannitol Salt Agar
Selectively supports the growth of gram positive staphylococcus, enterococcus, listeria, and micrococcaceae and inhibits streptococcus and gram negatives
S. Aureus on Mannitol salt
Ferments Mannitol and turns agar from red to yellow
S. epidermidis on Mannitol salt
Does not ferment and agar remains the same
S. aureus on purple agar base
Turns agar yello
Beta hemolysis
Complete hemolysis, dangerous species, turns blood agar clear
Alpha hemolysis
Incomplete hemolysis, turns agar green
Gamma hemolysis
No hemolysis or change in media
Staphyloxanthin
Carotenoid pigment in S. aureus indicating more pathogenicity
Catalase
Test for antioxidant, Staphylococcus will be positive
Coagulase
Causes serum to clot, positive strains are more pathogenic
Staph Habitat
Commensal in skin and tubular orifices
Risk of Staph Infection
Other infection, shearing in sheep, haircut in dogs, or nutritional imbalance
Body structures for adhesion
Cell wall, protein A, binding factors, and capsule or slime layer