Unit 2- Coliforms

Question 1

CEEK

Answer 1

Citrobacter, enterobacter, klebsiella

Question 2

Coliforms

Answer 2

All lactose fermenters

Question 3

TSI Test

Answer 3

Black from H2S Citrobacter and yellow from CO2 Enterobacter and Klebsiella

Question 4

Urease Test

Answer 4

Enterobacter negative and Klebsiella positive

Question 5

Important Klebsiella

Answer 5

K aerogenes, K pneumoniae, and K oxytoca

Question 6

Klebsiella

Answer 6

Gram negative, lactose fermenter, thick capsule, mucoid colonies, non motile, urease positive

Question 7

Klebsiella Serotypes

Answer 7

Various LPS O antigens and capsule K antigens

Question 8

K. pneumoniae Sizes

Answer 8

Classical and hypercapsulated hypervirulent are larger

Question 9

String Test

Answer 9

Hyperviscous colony indicates hypervirulent strain

Question 10

Klebsiella Surface Structures

Answer 10

Capsule, LPS, adherence factors, biofilm, outer membrane proteins

Question 11

Klebsiella Enzymes

Answer 11

Siderophores, urease

Question 12

Klebsiella Toxins

Answer 12

Colibactin interferes with host cycle, DNA damage, causes colorectal cancer

Question 13

Klebsiella Plasmids

Answer 13

5, leader of multidrug resistance

Question 14

Klebsiella Habitat

Answer 14

Mouth, skin, vulva, teats, GI, respiratory, fecal contamination

Question 15

Klebsiella Transmission

Answer 15

Normal flora or oral-fecal route

Question 16

Klebsiella pneumoniae

Answer 16

Lower respiratory tract pneumonia and mastitis

Question 17

Klebsiella oxytoca

Answer 17

Colitis, sepsis, mastitis

Question 18

Klebsiella Disease

Answer 18

Involves tubular tract: GI, UTI, pneumonia, mastitis, liver abscess, and meningitis

Question 19

Klebsiella Diagnosis

Answer 19

Gram negative, oxidate positive, lactose fermenter, CO2 on TSI, motile, urease

Question 20

Enterobacter

Answer 20

Lactose fermenter, motile, urease negative

Question 21

Enterobacter Culture

Answer 21

MacConkey or fecal odor on blood agar

Question 22

Enterobacter Habitat

Answer 22

Digestive tract, vulva, oral cavity

Question 23

Enterobacter Surface Structures

Answer 23

Flagella, type III secretion system

Question 24

Enterobacter Enzymes

Answer 24

Alpha hemolysis, pore forming cytotoxins, siderophores

Question 25

Enterobacter cloacae MDR

Answer 25

ESBL genes and plasmids

Question 26

Enterobacter Disease

Answer 26

UTI, intra-abdominal infection, pneumonia, bacteremia, wounds

Question 27

Enterobacter Diagnosis

Answer 27

Gram negative, oxidase, lactose fermenter, CO2 gas on TSI, motile, flagella

Question 28

Citrobacter

Answer 28

Gram negative rod, motile, slow lactose fermenter, H2S gas on TSI, citrate as carbon source, urease positive, O H and K antigens

Question 29

Citrobacter Habitat

Answer 29

Digestive tract, vulva, and oral cavity

Question 30

Citrobacter Virulence

Answer 30

Adhesins, motility, biofilm, immunavoidance, toxins, nutrient acquisition, type III secretion system

Question 31

Citrobacter Infection

Answer 31

Nosocomial

Question 32

Citrobacter Disease

Answer 32

Enteritis, UTI, endocarditis, pneumonia, brain abscess, septicemia, wound infection

Question 33

Citrobacter Bulla Formation

Answer 33

Rare

Question 34

Citrobacter Reptiles

Answer 34

Associated with turtles, cause septicemic cutaneous ulcerative disease, petechiae on shell, anorexia, liver necrosis, and lethargy

Question 35

Citrobacter Fish

Answer 35

Causes ulcer and muscle necrosis