Unit 2- Waterborne

Question 1

Enterobacteriaceae

Answer 1

Edwardsiella and pleisomonas

Question 2

Edwardsiella tarda

Answer 2

Facultative anaerobe, gram negative rod, motile, terrestrial and aquatic, opportunistic pathogen, zoonotic

Question 3

E tarda Virulence Factors

Answer 3

Flagella, LPS, fimbriae, hemolysin, proteinase, T3SS, T6SS, serum resistance, capsule, and quorum sensing

Question 4

E tarda Intracellular Behavior

Answer 4

Enters cells in vacuoles, replicates, and destroys membrane

Question 5

Fish Edwardsiellosis

Answer 5

E tarda, E piscicida, E ictaluri, and E anguillarum cause granuloma, skin ulceration, and ascites in commercial fish

Question 6

Human Edwardsiellosis

Answer 6

E tarda causes diarrhea and bacteremia after eating contaminated freshwater fish

Question 7

Cow Typhlocolitis

Answer 7

E tarda causes greenish fluid, fecal material, and fibrin deposition on peritoneum, ruptures on cecum, and petechiae in colon

Question 8

E. tarda Culture

Answer 8

MacConkey Agar

Question 9

E tarda Diagnosis

Answer 9

Hemolytic on blood agar, pale colorless on macconkey, no growth on brilliant green, reddish black center on CLD, oxidase negative, catalase positive, agglutination test, ELISA

Question 10

E tarda Treatment

Answer 10

Antibiotics, quarantine, and water quality in fish and natural cure, antibiotics, and debridement in humans

Question 11

Pleisomonas shigelloides

Answer 11

Gram negative, facultative anaerobic rod, oxidase positive, motile, zoonotic

Question 12

Human P shigelloides Infection

Answer 12

Ingestion of contaminated water and seafood, enterotoxins, LPS, hemolysin, and iron acquisition system cause watery diarrhea and bacteremia

Question 13

Shigelloides Systemic Infection

Answer 13

Hemorrhagic congestion and ascites, decolorization of liver, air bubbles in stomach, splenomegaly

Question 14

P shigelloides Diagnosis

Answer 14

Blood agar, colorless on MacConkey, green colonies on Hektoen enteric because no carb use, catalase, oxidase, and indole positive

Question 15

P shigelloides Treamtnet

Answer 15

Resistant to penicillin, treatment may not be necessary

Question 16

Aeromonas

Answer 16

Gram negative, facultative anaerobe, straight rod, normal flora of fish

Question 17

Human Pathogen Aeromonas

Answer 17

A hydrophila is motile and mesophilic

Question 18

Fish Pathogen Aeromonas

Answer 18

A salmonicida is non motile and psychrophilic

Question 19

Aeromonas Virulence Factors

Answer 19

Type 1 and IV pili, flagella, exoenzymes, hemolysin, enterotoxins, T3SS, T6SS, quorum sensing, capsule and slime layer

Question 20

Aeromonas hydrophila

Answer 20

Motile, mesophilic, cause hemorrhagic septicemia and skin lesions in fish and diarrhea and septicemia in humans

Question 21

Aeromonas salmonicida

Answer 21

Non motiel, psychrophilic, furunculosis in salmonid fish, ulcer in goldfish

Question 22

Aeromonas Diagnosis

Answer 22

Hemolytic, catalase positive, oxidase positive, hydrophila grows in warm temp, large colonies, and motile, salmoncida grows in cold temp, small colonies, and non motile

Question 23

Aeromonas Treatment

Answer 23

Resistant to penicillins

Question 24

Vibrio

Answer 24

Gram negative, facultative anaerobe, curved rod, motile, halophilic

Question 25

Halophilic

Answer 25

Present in salty water

Question 26

Vibrio cholerae

Answer 26

Human pathogen, cholera toxin causes watery diarrhea, dehydration, and death

Question 27

Vibrio parahaemolyticus

Answer 27

Cause of food poisoning, thermostable direct hemolysin, T3SS, T5SS, flagella, adhesin, and LPS

Question 28

V parahaemolyticus Diagnosis

Answer 28

Grown on selective media, oxidase positive, catalase positive, non hemolytic on sheep blood, hemolytic on human wagatsuma agar, yellow colonies on mannitol salt, green on TCBS agar

Question 29

Vibrio metschnikovii

Answer 29

Cholera in birds, hemolysin and verotoxin, hemolytic, poor growth on MacConkey, oxidase negative, catalase positive

Question 30

Vibrio anguillarum

Answer 30

Saltwater eel and fish pathogen, skin necrosis and hemorrhagic septicemia, iron uptake, hemolysin, metalloproteases, and adhesion structures

Question 31

Metalloprotease

Answer 31

Toxic to host cells

Question 32

V anguillarum Diagnosis

Answer 32

Hemolytic, yellow on TCBS