Unit 1- Identification Flashcards
Streaking
Identify colony growth
Serial Dilutions
Calculate number of bacteria
MacConkey’s Agar
Selective and differential, prevents gram + from growing and colors lactose utilizers red
Colony Characteristics
Surface texture, colony shape, elevation, margin, size, color
Microscopic Characteristics
Cell size, shaoe, grouping, gram stain, spores
Wet Mount Motility
Direct microscopic observation for motility
Soft Agar Stab
Motility culture medium, incubation allows determination of motility
H Antigens
Flagella proteins
O antigens
LPS
K Antigens
Capsule polysaccharides or protein
Slide Agglutination
Determines if cells group together
Common Serological Assays
ELISA, latex agglutination, western blot
Durham Tube
Detects carb utilization, fermentation lowers pH and turns liquid yellow
Triple Sugar Iron Medium
Used to differentiate enteric bacteria based on ability to ferment sugars, produce H2S, and/or produce gas
Non-Fermentor TSI Reaction
Alkaline sland and deep
Non Lactose Fermentor TSI Reaction
Alkaline slant, acid deep
Lactose Fermentor TSI Reaction
Acid slant and acid deep
Bromthymol Blue
pH indicatior in O-F medium
Protein Deamination
Deaminase creates oxidizers that raise pH
Protein Desulfurylation
Desulfurylase produces insuluble black pt
Protein Decarboxylation
Decarboxylase produces alkaline pH
Enterotube
Each compartment has a specific substrate in an agar base to identify enteric bacteria
API 20E System
20 tests overcome individual strain variation
