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Bacteriology > Unit 1- Identification > Flashcards

Unit 1- Identification Flashcards

1
Q

Streaking

A

Identify colony growth

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2
Q

Serial Dilutions

A

Calculate number of bacteria

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3
Q

MacConkey’s Agar

A

Selective and differential, prevents gram + from growing and colors lactose utilizers red

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4
Q

Colony Characteristics

A

Surface texture, colony shape, elevation, margin, size, color

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5
Q

Microscopic Characteristics

A

Cell size, shaoe, grouping, gram stain, spores

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6
Q

Wet Mount Motility

A

Direct microscopic observation for motility

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7
Q

Soft Agar Stab

A

Motility culture medium, incubation allows determination of motility

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8
Q

H Antigens

A

Flagella proteins

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9
Q

O antigens

A

LPS

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10
Q

K Antigens

A

Capsule polysaccharides or protein

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11
Q

Slide Agglutination

A

Determines if cells group together

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12
Q

Common Serological Assays

A

ELISA, latex agglutination, western blot

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13
Q

Durham Tube

A

Detects carb utilization, fermentation lowers pH and turns liquid yellow

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14
Q

Triple Sugar Iron Medium

A

Used to differentiate enteric bacteria based on ability to ferment sugars, produce H2S, and/or produce gas

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15
Q

Non-Fermentor TSI Reaction

A

Alkaline sland and deep

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16
Q

Non Lactose Fermentor TSI Reaction

A

Alkaline slant, acid deep

17
Q

Lactose Fermentor TSI Reaction

A

Acid slant and acid deep

18
Q

Bromthymol Blue

A

pH indicatior in O-F medium

19
Q

Protein Deamination

A

Deaminase creates oxidizers that raise pH

20
Q

Protein Desulfurylation

A

Desulfurylase produces insuluble black pt

21
Q

Protein Decarboxylation

A

Decarboxylase produces alkaline pH

22
Q

Enterotube

A

Each compartment has a specific substrate in an agar base to identify enteric bacteria

23
Q

API 20E System

A

20 tests overcome individual strain variation

Bacteriology (44 decks)

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