Unit 3- Mycoplasma Flashcards
PPLO
Pleuropneumonia-like organisms
Mycoplasma
Pleiomorphic shape, lack cell wall, electron dense core terminal, motile
Core Terminal
At one end of the cell, allows attachment onto host cells, gliding motility, cell division
Mycoplasma Enzymes
RNA polymerase makes them prone to mutation
Mycoplasma Cell Surface
Glycolipids, lipoglycans, lipoprotein, capsule, host derived cholesterol, adhesins
Mycoplasma Secretion
H2O2, superoxide, nuclease, immunoglobulin protease, antioxidant enzymes, sialidases, hyaluronidases
Mycoplasma Culture
Slow fastidious growth, mycoplasma selective media
Mycoplasma Agar
Beef heart, yeast autolysate, nucleic acid precursors, penicillin to prevent gram positive, thallium acetate to prevent gram negative, serum
Mycoplasma Growth on Agar
Slow growing allows for chronic disease, gliding motility gives fried egg appearance
Small Colony
Mycoplasma mycoides subsp mycoides infecting cattle
Large Colony
Mycoplasma mycoides subsp mycoides infecting goat
Mycoplasma Virulence Factors
Cell surface molecules for antigen variation and mimicry, secreted enzymes, manipulation of host cells, parasitism outside host cells
Capping
Incorporation of host antigens into Mycoplasma
Mycoplasma Infection
Elicits inflammation, MAKePSR syndrome, cancer, autoimmunity
Mycoplasma Cancer
Inhibits P53 tumor suppressor protein
Intracellular Mycoplasma
Change host cell morphology and genetics, cause cancers
Extracellular Mycoplasma
Intimately attached to membrane surface
Mycoplasma Transmission
Airborne, contact, sexual, ingestion, transovarian in birds
Mycoplasma Tropism
Infects mucosal tracts and other body parts in all vertebrates with some host specificity
MAKePSR Syndrome
Mastitis, arthritis, keratoconjunctivitis, pneumonia, septicemia, reproductive disorder
Bovine Mycoplasma Species
Mycoplasma bovis and mycoides subsp mycoides small colony variant
Bovine Mycoplasma Symptoms
Arthritis, extended neck with legs far apart, coughing, ocular and nasal discharge, pleural fluid, thickening of lung, lung adherence to chest wall
CBPP
Contagious bovine pleuropneumonia, can cause a trade ban
CCPP
Contagious caprine pleuropneumonia, can cause a trade ban
Sheep and Goat Mycoplasma Species
Mycoplasma agalactiae, capricolum, mycoides, ovipneumonaie
Sheep and Goat Mycoplasma Symptoms
Mastitis, mammary atrophy, high fever, cough, wide stance, granular lung
Swine Mycoplasma Species
Mycoplasma hyopneumoniae, hyorhinis, and hyosynoviae
Swine Mycoplasma Symptoms
Lung hepatization, cranioventral lung consolidation, collapse of lung lobes
Poultry Mycoplasma Species
Mycoplasma gallisepticum, synoviae, and meleagridis
Poultry Mycoplasma Symptoms
Airsacculitis, sinusitis, swollen sinuses, periorbital swelling, swollen foot, arthritis
Horse Mycoplasma Species
Mycoplasma felis, equirhinitis, equipharyngis
Horse Mycoplasma Symptoms
Pleuritis
Pet Mycoplasma Species
Mycoplasma cynos and felis
Pet Mycoplasma Symptoms
Pneumonia, conjunctivitis
Haemobartonella canis
Mycoplasma haemocanis, causes severe hemolytic anemia in dog
Haemobartonella felis
Mycoplasma haemofelis, causes severe hemolytic anemia in cat
Mycoplasma Pathology
Thickening and fibrosis of lung, hepatization of lung, marbling, granuloma, necrotic encapsulated lesions, straw colored fluid on thoracic cavity, enlarged joints
Iceberg Concept
High mortality, high morbidity, recovered animals can be carriers
Mycoplasma Diagnosis
Fried egg colonies on agar, serology, PCR
Mycoplasma Treatment
Intrinsic drug resistance, sequestration in granuloma, cull infected animals