Unit 2- Pseudomonas and Burkholderia

Question 1

Pseudomonas aeruginosa

Answer 1

Gram neg aerobic rods, polar flagella, fruity grape like smell, diffusible pigments, opportunistic pathogen in many environments, MDR

Question 2

P aeruginosa Toxins

Answer 2

Exotoxin A, phospholipase C, pyocyanin, pyoverdine, degradative enzymes

Question 3

P aeruginosa Attachment

Answer 3

Pili, flagella, LPS

Question 4

P aeruginosa Biofilm Formation

Answer 4

Mucoid EPS alginate

Question 5

P aeruginosa Clinical Signs

Answer 5

Fleece rot, pyoderma in dogs, otitis, ulcerative keratitis, pneumonia, bacteremia, mastitis, stomatitis, oxygenated organs

Question 6

P aeruginosa Diagnosis

Answer 6

Aerobic culture, oxidase and catalase positive, pyocyanin on nutrient agar, non lactose fermenter, beta hemolysis

Question 7

P aeruginosa Control

Answer 7

Vaccines for mink and chinchilla

Question 8

Pseudomonas fluorescens

Answer 8

Gram neg, motile, aerobic rods, non glucose fermenters, produce pyoverdine, cause skin ulcer and septicemia in reptile and fish

Question 9

Pyoverdine

Answer 9

Fluorescent pigment

Question 10

Burkholderia mallei

Answer 10

Gram neg, non motile, aerobic coccobacillus, facultative intracellular, causes glanders in equids, bioterrorism agent

Question 11

B mallei Virulence Factors

Answer 11

Capsule, LPS, secretion systems, quorum sensing, adhesion proteins, fimbriae

Question 12

Glanders

Answer 12

Endemic zoonotic disease in equines, nodules and ulcers on skin and respiratory, pulmonary, cutaneous, and nasal form

Question 13

Equine Farcy

Answer 13

Cutaneous form of Glanders

Question 14

B mallei Diagnosis

Answer 14

Glycerol media, aerobic culture compliment fixation test, ELISA

Question 15

Mallein Test

Answer 15

Hypersensitivity tear test in horses used to test for Glanders

Question 16

B mallei Control

Answer 16

Cleaning and disinfection, euthanasia

Question 17

Burkholderia pseudomallei

Answer 17

Gram neg, aerobic, motile, safety pin shaped bacillus, bipolar staining, facultative intracellular, bioterrorism agent

Question 18

B pseudomallei Infection

Answer 18

Causes abscess in many organs, Meliodosis, Whitmore’s disease, and pseudoglanders

Question 19

B pseudomallei Virulence Factors

Answer 19

Capsule, LPS, secretion systems, quorum sensing, type IV pili, exotoxins, protease

Question 20

B pseudomallei Clinical Signs

Answer 20

Lung abscess and pneumonia in sheep, mastitis in goat, lesions on spleen in pig, pseudoglanders in horse, abscess in organs of dog and cat, pneumonia in cow, and abscess and pneumonia in human

Question 21

B pseudomallei Diagnosis

Answer 21

Musty odor, grey and non hemolytic on blood agar, indirect hemagglutination