P28 - Respiratory Cytology Flashcards

1
Q

pre-sampling test for cytology (2)

A
  • clotting function (sodium citrate)

- CBC

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2
Q

sample collection for cytology in what tube

A
  • EDTA
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3
Q

sample collection for culture and sensitivity in what tube

A
  • plain (sterilin) tubes
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4
Q

nasal swabs are useful for what kind of discharge

A
  • serous or purulent for direct smear
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5
Q

what technique is useful in identifying fungal organisms (cryptococcus) in cats

A
  • nasal swabs
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6
Q

what results/test are useless after a nasal lavage (4)

A
  • sediment smear - due to dilution
  • protein assays - due to dilution
  • TNCC - don’t know original concentration
  • Culturing
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7
Q

microscopic findings in normal nasal lavage (4)

A
  • RBCs
  • ciliated columnar epithelium
  • macrophages
  • normal bacteria (somonsiella)
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8
Q

somonsiella

A
  • normal bacteria in nasal tract

- thick with striations

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9
Q

microscopic findings in infectious rhinitis (4)

A
  • mixed inflammatory cells
  • mucin
  • microorganisms
  • dysplastic changes
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10
Q

during inflammation there is an increase in what type of cell

A
  • goblet cells
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11
Q

microscopic findings in non-infectious rhinitis (3)

A
  • unresponsive to antibiotics
  • foreign body
  • neoplasia (non-deg neutrophils)
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12
Q

tracheobronchial lavage (TBL)

A
  • sterile saline or PBS introduced into lower trachea space

- samples lung, bronchi and trachea

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13
Q

bronchoalveolar lavage (BAL)

A
  • sterile saline or PBS introduced into bronchoalveolar space
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14
Q

how to prevent oropharyngeal contamination

A
  • transtracheal lavage
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15
Q

microscopic finding in normal BAL or TBL (8)

A
  • ciliated columnar epithelium
  • simonsiella
  • few neutrophils
  • alveolar macrophages
  • occasional goblet cells
  • few lymphocytes
  • eosinophils - healthy cats
  • few mast cells
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16
Q

microscopic findings in acute sterile inflammation (2)

A
  • non-degenerate neutrophils

- no microorganisms

17
Q

microscopic findings in acute septic inflammation (2)

A
  • degenerate neutrophils

- intracellular microorganisms

18
Q

microscopic findings in chronic inflammation (3)

A
  • mixed population of inflammatory cells
  • mucus
  • squamous cell metaplasia
19
Q

process of chronic obstructive airway (pulmonary) disease (5)

A
  • persistent irritation to bronchioles
  • proliferation of goblet cells
  • increased mucin production
  • obstruction of airway
  • cough, exercise intolerance
20
Q

causes of eosinophilic inflammation (3)

A
  • D. immitis - dogs
  • Dictyocaulus - cattle
  • Asthma - cats
21
Q

what is seen in acute cases of exercise-induced pulmonary hemorrhage

A
  • erythrophages
22
Q

what is seen in chronic cases of exercise-induced pulmonary hemorrhage

A
  • hemosiderophages
23
Q

lung neoplasms that exfoliate well are detached in what

A
  • BAL