P27 - CSF Fluid Flashcards
purpose of analysis of CSF
- evaluation of neurologic disease
CSF flows in what direction
- cranial to caudal
CSF must be analyzed within ___ of collection
- 1 hour
what tube is preferred for CSF collection
- red top
problems with EDTA for CSF collection (3)
- increases protein concentration
- causes cell lysis
- dilutes TNCC and protein in small samples
what is added to stabilize CSF sample
- BSA or fetal calf serum
what is measured before BSA or fetal calf serum is added to CSF and why
- protein
- serum is rich in protein
laboratory findings in normal CSF (5)
- colorless
- transparent
- water-like
- low protein
- low TNCC
xanthochromia
- presence of hemoglobin in CSF
- pinkish to orange to yellowish color
hematorrhachis
- hemorrhage in vertebral column
- pinkish-red to orange discoloration
bilirrhachia
- hemoglobin present for 2 days
- bilirubin
turbidity/pleocytosis of CSF
- high TNCC
causes of pleocytosis
- inflammation and microorganisms
- neoplasia
- hemorrhage
horses have higher concentrations of what in CSF
- proteins - 80mg/dl
gel or clot formation of CSF is indicative of
- inflammation
where does CSF have higher protein concentrations and why
- lumbosacral samples
- more water removed
effects of drop of blood contamination of CSF on RBC, TNCC, and protein
- increases RBC by 12,000/uL
- increases TNCC by 200/uL
- increases protein by 100mg/dl
cytologic/albuminocytologic dissociation
- high protein without a corresponding increase in TNCC
causes of cytologic/albuminocytologic dissociation
- compression from space occupying lesion
- pathology deep in parenchyma and can’t reach CSF
- BBB pathologic cells not superficial enough to slough into CSF
further unique CSF tests (3)
- [glucose]
- creatine kinase
- antibody titers
hypoglycorrhacia and causes
- low CSF glucose
- utilized by bacteria in CSF, inflammatory cells, RBC
- hypoglycemia
hyperglycorrhacia and causes
- high CSF glucose
- hyperglycemia
- increase permeability of BBB
[CSF glucose] is about what % of [plasma glucose]
- 60-80%
what is increased in case of damage to nerve cells
- creatine kinase
