P21 - Principles of Diagnostic Cytology

Question 1

what is diagnostic cytology

Answer 1

• aspect of clinical pathology that deals with nucleated cell populations in cutaneous masses, internal organs, accumulated fluid

Question 2

types of inflammatory cytology samples (2)

Answer 2

• septic

- non-septic

Question 3

types of neoplastic cytology samples (3)

Answer 3

• epithelial
• spindle
• round

Question 4

how to properly perform a fine needle aspirate (FNA)

Answer 4

• do not attach syringe in first place

- redirect needle more peripherally

Question 5

gauge needle to use for a FNA

Answer 5

• 22 to 25

Question 6

indications to perform a FNA

Answer 6

• cutaneous masses of unknown origin
• enlarged lymph nodes
• masses on internal organs

Question 7

how to properly perform a touch imprint (impression) smear

Answer 7

• cut surface must be free of “juices” or blood

- several imprints on same slide

Question 8

fluids should be collected in what type of tube

Answer 8

• EDTA

Question 9

lavages or washes (3)

Answer 9

• tracheal
• bronchoalveolar
• bladder or vaginal

Question 10

what makes a sample non-diagnostic (6)

Answer 10

• placing close to formalin
• refrigeration smears
• using contaminated samples
• sampling and smearing techniques
• breakage or leakage during shipment
• staining