Oral environment 1 Flashcards

1
Q

Components of oral fluid

A

saliva, gingival crevicular fluid, oral bacteria, food debris, epithelial cells

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2
Q

2 overall functions of oral fluid

A

protection and digestion

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3
Q

Examples of the protective function of oral fluids

A

buffering, antimicrobial, remineralisation, cleansing, mucosal protection

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4
Q

Examples of the digestive function of oral fluids

A

taste, digestive enzymes, bolus lubrication for mastication and deglutination

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5
Q

What is a prominent symptom for patients with salivary gland disease?

A

xerostomia (dry mouth)

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6
Q

Effects of reduced salivary flow

A

increased incidence of caries

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7
Q

Which tooth surfaces are most at risk of caries due to reduced salivary flow?

A

Buccal and labial

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8
Q

What type of glands are salivary glands?

A

Exocrine

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9
Q

Structure of salivary glands

A

compound, tubulo-acinar glands

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10
Q

What are the major (paired) salivary glands

A

parotid, submandibular, sublingual

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11
Q

Where are the minor salivary glands located?

A

Buccal, labial, lingual, palatal (cheeks, lips, tongue, palate)

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12
Q

Which histological layer are minor salivary glands found in?

A

(glandular tissue found in) lamina propria

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13
Q

Names of different ducts found in salivary glands

A

Intercalated ducts emerge from the acini and join to into the striated duct. Striated ducts from multiple bundles of acini join into the single collecting duct.

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14
Q

Microscopic appearance of serous acini

A

Stain pink with H+E, rounded nuclei which are more centrally located in each cell

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15
Q

Microscopic appearance of mucous acini

A

foamy cytoplasm, flattened, peripherally-located nuclei (full of vesicles containing mucin)

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16
Q

Microscopic appearance of mixed acini

A

mucous and serous acini can be seen

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17
Q

Type of secretion from parotid gland

A

serous

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18
Q

type of secretion from submandibular gland

A

seromucous

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19
Q

Type of secretion from sublingual gland

A

mucous

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20
Q

Type of secretions from minor salivary glands

A

Buccal, labial, palatal minor salivary glands all have mucous secretions. Lingual salivary glands secrete serous and mucous secretions (but the acini are not mixed)

21
Q

Name of fluid lining the gingival sulcus/crevice

A

Gingival crevicular fluid (GCF)

22
Q

What will cause an increase in gingival crevicular fluid (GCF)?

A

Inflammation (e.g. gingivitis) - GCF is probably an inflammatory exudate

23
Q

Name of the epithelium in the gingival sulcus/crevice

A

sulcular/crevicular epithelium

24
Q

Name of the epithelium that attaches to the tooth

A

junctional epithelium

25
Q

What is the rate of salivary flow when sleeping?

A

0.03 ml/min

26
Q

What is the salivary flow rate when resting but awake?

A

0.3 ml/min

27
Q

What is the salivary flow rate when eating?

A

3.0 ml/min

28
Q

Average total salivary flow

A

620 ml

29
Q

% contributions of the salivary glands when asleep

A

submandibular 70%
sublingual 15%
Minor glands 15%
parotid 0%

30
Q

% contributions of salivary glands when awake but resting

A

submandibular 72%
parotid 20%
sublingual 4%
minor glands 4%

31
Q

% contributions of salivary glands when stimulated (eating)

A

Parotid 50%
Submandibular 40%
Minor glands 8%
Sublingual 2%

32
Q

Factors that affect unstimulated salivary flow rate

A

hydration, previous stimulation, circannual rhythms (seasons), medications, salivary gland disease

33
Q

Composition of saliva

A

inorganic - water (99.5%) and ions (0.2%)
organic - proteins (0.3%), very little carbohydrate or lipid

34
Q

As salivary flow rate increases, how does the composition of ions change in saliva?

A

Na+, Cl- and HCO3- concentrations increase, K+ concentration decreases

35
Q

Why do Na+ and Cl- concentrations in saliva increase as flow rate increases?

A

Na+ and Cl- are the driving forces for salivary secretion (water follows by osmosis)

36
Q

Significance of increased HCO3- concentration at higher flow rates

A

HCO3- is an effective buffer of plaque acids around pH 7 (important when eating)

37
Q

Which ions are present in saliva?

A

Fluoride, calcium, phosphate, thiocyanate, bicarbonate, (sodium, chloride, potassium)

38
Q

Function of fluoride in saliva

A

Forms fluorapatite, antibacterial (acts on H pump), promotes remineralisation

39
Q

Function of calcium and phosphate ions in saliva

A

remineralisation (also phosphate is a buffer)

40
Q

Function of thiocyanate (SCN) in saliva

A

antibacterial

41
Q

function of bicarbonate and phosphate in saliva

A

buffer

42
Q

List of salivary buffers

A

bicarbonate, phosphates, proteins, bacterial NH3

43
Q

When is bicarbonate most effective as a buffer?

A

At high salivary flow rates when HCO3- concentration is greatest

44
Q

When are phosphates most effective at buffering?

A

At rest, near pH 7

45
Q

When are proteins most effective at buffering?

A

Limited due to main buffering occurring at pH<5

46
Q

How is NH3 produced?

A

By bacteria: urea -> CO2 + NH3 catalysed by urease

47
Q

How is saliva secreted?

A

Na+ is able to move by facilitated diffusion from the blood into the intercalated duct via acini cells. Cl- moves into the acini cells via Cl-/K+ pump. ACh causes depolarisation of the acini cells which triggers the release of Ca2+. Ca2+ bind to Cl- receptors opening them so Cl- can diffuse into the duct. Water follows Na+ and Cl- into the duct lumen.

48
Q

Equation for phosphate buffer

A

H2PO4 - <-> H+ + HPO4 2-

49
Q

How does NH3 act as a buffer?

A

NH3 + H+ -> NH4+