Micro-Anaerobic Bacteria Flashcards
1
Q
- Obligate anaerobes, facultative anaerobes, and
microaerophiles are terms referring to bacteria
that require:
A. Increased nitrogen
B. Decreased CO2
C. Increased O2
D. Decreased O2
A
D. Decreased O2
2
Q
- Which of the following most affects the
oxidation–reduction potential (Eh or redox
potential) of media for anaerobic bacteria?
A. O2
B. Nitrogen
C. pH
D. Glucose
A
C. pH
3
Q
- Which of the following is the medium of choice
for the selective recovery of gram-negative
anaerobes?
A. Kanamycin–vancomycin (KV) agar
B. Phenylethyl alcohol (PEA) agar
C. Cycloserine–cefoxitin–fructose agar (CCFA)
D. THIO broth
A
A. Kanamycin–vancomycin (KV) agar
4
Q
- Anaerobic bacteria are routinely isolated from
all of the following types of infections except:
A. Lung abscesses
B. Brain abscesses
C. Dental infections
D. Urinary tract infections
A
D. Urinary tract infections
5
Q
- Methods other than packaged microsystems used
to identify anaerobes include:
A. Antimicrobial susceptibility testing
B. Gas–liquid chromatography (GLC)
C. Special staining
D. Enzyme immunoassay
A
B. Gas–liquid chromatography (GLC)
6
Q
- Which broth is used for the cultivation of
anaerobic bacteria in order to detect volatile fatty
acids as an aid to identification?
A. Prereduced peptone–yeast extract–glucose (PYG)
B. THIO broth
C. Gram-negative (GN) broth
D. Selenite (SEL) broth
A
A. Prereduced peptone–yeast extract–glucose (PYG)
7
Q
- A gram-positive spore-forming bacillus growing on
sheep-blood agar anaerobically produces a double
zone of β-hemolysis and is positive for lecithinase.
What is the presumptive identification?
A. Bacteroides ureolyticus
B. Bacteroides fragilis
C. Clostridium perfringens
D. Clostridium difficile
A
C. Clostridium perfringens
8
Q
- Egg yolk agar is used to detect which enzyme
produced by Clostridium species?
A. Lecithinase
B. β-Lactamase
C. Catalase
D. Oxidase
A
A. Lecithinase
9
Q
- Which of the following organisms will display
lipase activity on egg yolk agar?
A. Clostridium botulinum
B. Clostridium sporogenes
C. Clostridium novyi (A)
D. All of these options
A
D. All of these options
10
Q
- Which spore type and location is found on
Clostridium tetani?
A. Round, terminal spores
B. Round, subterminal spores
C. Ovoid, subterminal spores
D. Ovoid, terminal spores
A
A. Round, terminal spores
11
Q
- Gram-positive bacilli recovered from two blood
cultures from a 60-year-old diabetic patient gave
the following results:
Spores seen = Neg Hemolysis = + (double zone)
Motility = Neg
Lecithinase = +
Volatile acids by GLC (PYG) = acetic acid (A) and butyric
acid (B)
What is the most likely identification?
A. Clostridium tetani
B. Clostridium perfringens
C. Clostridium novyi (B)
D. Clostridium sporogenes
Microbiology/Evaluate lab
A
B. Clostridium perfringens
12
Q
- Which mechanism is responsible for botulism in
infants caused by Clostridium botulinum?
A. Ingestion of spores in food or liquid
B. Ingestion of preformed toxin in food
C. Virulence of the organism
D. Lipase activity of the organism
A
A. Ingestion of spores in food or liquid
13
Q
- The classic form of foodborne botulism is
characterized by the ingestion of:
A. Spores in food
B. Preformed toxin in food
C. Toxin H
D. All of these options
A
B. Preformed toxin in food
14
Q
- Which test is performed in order to confirm an
infection with Clostridium botulinum?
A. Toxin neutralization
B. Spore-forming test
C. Lipase test
D. Gelatin hydrolysis test
A
A. Toxin neutralization
15
Q
- Which Clostridium spp. causes pseudomembranous
colitis or antibiotic-associated colitis?
A. C. ramosum
B. C. difficile
C. C. perfringens
D. C. sporogenes
A
B. C. difficile
