Immunohematology- Serological Testing of Blood Products

Question 1

1. What protocol is followed when screening whole
   blood donors for HIV-1 RNA?
   A. Pools of 10 are tested; if the pool is nonreactive,
   donors are accepted
   B. Pools of 20 are tested; if the pool is reactive,
   samples are tested individually
   C. Pools of up to 16 donors are tested; if pool is
   reactive, individual samples are screened
   D. All donors are screened individually; if samples
   are reactive, blood is discarded

Answer 1

C. Pools of up to 16 donors are tested; if pool is
reactive, individual samples are screened

Question 2

2. Currently, nucleic acid amplification testing
   (NAT) testing is performed to detect which
   viruses?
   A. HIV and HTLV-1
   B. HTLV I/II
   C. HIV, HCV, and WNV
   D. HIV, HBV, and WNV

Answer 2

C. HIV, HCV, and WNV

Question 3

3. John comes in to donate a unit of whole blood
   at the collection center of the community
   blood supplier. The EIA screen is reactive for
   anti-HIV-1/2. The test is repeated in duplicate
   and is nonreactive. John is:
   A. Cleared for donation
   B. Deferred for 6 months
   C. Status is dependent on confirmatory test
   D. Deferred for 12 months

Answer 3

A. Cleared for donation

Question 4

4. What marker is the first to appear in hepatitis B
   infection?
   A. Anti-HBc (IgM)
   B. HbsAg
   C. Anti-HBs
   D. Anti-HBc (IgG)

Answer 4

B. HbsAg

Question 5

5. What marker indicates immunity to hepatitis B
   infection?
   A. Anti-HBc (IgM)
   B. HBsAg
   C. Anti-HBs
   D. Anti-HBc (IgG)

Answer 5

C. Anti-HBs

Question 6

6. An EIA screening test for HTLV I/II was performed
   on a whole-blood donor. The results of the EIA
   were repeatedly reactive but the confirmatory test
   was negative. On the next donation, the screening
   test was negative by two different EIA tests. The
   donor should be:
   A. Accepted
   B. Deferred
   C. Told that only plasma can be made from his
   donation
   D. Told to come back in 6 months

Answer 6

A. Accepted

Question 7

7. A unit tests positive for syphilis using the rapid
   plasma reagin test (RPR). The microhemagglutinin
   assay-Treponema pallidum (MHA-TP) on the
   same unit is negative. What is the disposition of
   the unit?
   A. The unit may be used to prepare components
   B. The donor must be contacted and questioned
   further; if the RPR result is most likely a false
   positive, then the unit may be used
   C. The unit must be discarded
   D. Cellular components may be prepared but must
   be irradiated before issue

Answer 7

A. The unit may be used to prepare components

Question 8

8. SITUATION: John Smith donated a unit of whole
   blood in May. Red blood cells made from the
   whole blood were transfused to a recipient of a
   community hospital in June with no apparent
   complications. The blood supplier notified the
   medical director of the hospital that the donor
   reported high-risk behavior with another male in
   April, although viral tests remain negative and the
   donor is healthy. What course of action should be
   taken?
   A. No action should be taken
   B. The recipient’s physician should be notified
   C. The recipient’s physician and the recipient
   should be notified
   D. The recipient should be notified

Answer 8

B. The recipient’s physician should be notified

Question 9

9. All of the following are required tests on donor
   blood, except:
   A. HBsAg
   B. Anti-CMV
   C. HIV-1
   D. Anti-HTLV I/II

Answer 9

B. Anti-CMV

Question 10

10. Which of the following bands would constitute a
    positive Western Blot for HIV?
    A. p24, gp41, p17
    B. p55, gp120, p51
    C. gp160, p31, p56
    D. p24, p30, p55

Answer 10

A. p24, gp41, p17