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resolving power or
resolution of the instrument.
Microscopes
resolving power of the unaided human
eye is approximately 0.2 mm.
Microscopes
The most used tool in the study of basic genetics
Compound Light Microscope/ Light Microscope
is one that contains only one
magnifying lens.
Simple Microscopes
- Also known as compound light microscope because it uses not
only one, but several lenses that are specifically arranged to
magnify an image.
LIGHT MICROSCOPE
is the product of the
magnification of the eyepiece and the objective that is in use.
Magnification (Linear Magnification)-
generally used only when studying very minute parts
of a specimen like individual cells or parts of cells.
HPO and OIO-
studied under and LM and TEM microscope
are very thin slices of the cell tissue or organ under consideration.
Specimens or sections
contains more than one magnifying
lens.
Compound Microscopes
wavelength of visible light (~0.45
μm) that limits the size of objects that can
be seen.
Compound Microscopes
the compound light microscope is sometimes
referred to as a
brightfield microscope.
If the condenser is replaced with what is known as a darkfield
condenser, illuminated objects are seen against a dark
background or “dark field”; the microscope is then called a
darkfield microscope.
are used to observe unstained
living microorganisms.
Phase-contrast microscopes
There are two types of electron microscopes:
Transmission
and Scanning.
black and white photograph
Electron micrograph-
an electron gun
to fire a beam of electrons through an
extremely thin specimen
Transmission Electron Microscope
An image of the specimen is produced
on a phosphor-coated screen.
Transmission Electron Microscope
Electrons are bounced off the
surface of a specimen and the image
appears on a monitor.
Scanning Electron Microscope
This is used to observe the outer
surfaces of specimens.
Scanning Electron Microscope
first
instrument that
simultaneously reveals where
an RNA or DNA molecule
DNA MICROSCOPY
