lab prelim Flashcards
instrument that consists of at least two magnifying lenses to
produce an enlarged and focused image of a specimen, the ocular
and the objective
compound micr
The total magnification of any set of lenses is determined by
multiplying the magnification of the objective by the magnification of
the ocular.
compound microscope
This is the cylindrical tube
structure that holds the eyepiece.
draw tube
It consists of the compound lens at
the end of the draw tube, which usually
magnifies from 5 to 15 times. This is also
called the ocular, where the observer peeps
through to see the specimen.
eyepiece
This is bigger tube located below
the draw tube. It allows significant distance
between the lens of the eyepiece and the lens
of the objective.
body tube
This is the circular part that
protects the objectives from dust.
dust shield
This is the
rotating part below the dust shield,
which facilitates the use of the
objectives.
revolving nose piece
- Magnification Range: 4x to 10x
Low-power objective (LPO).
Observing larger specimens, getting a
general sample overview, and locating areas of interest
for higher magnification.
Low-power objective (LPO).
- A larger lens with a lower magnification power.
- Wider field of view (how much you can see at once).
- It is shorter in length compared to higher-power
lenses
Low-power objective (LPO).
- Magnification Range: 20x to 40x
Medium-power objective lens (MPO)
Typical Uses: More detailed examination of specimens,
studying cell structures and smaller microorganisms.
Medium-power objective lens (MPO)
- A moderate-sized lens with a medium magnification
power. - Provides a balance between magnification and
resolution. - More precise details compared to low-power lenses.
Medium-power objective lens (MPO)
- Magnification Range: 40x to 100x or more
High-power objective lens (MPO)
Typical Uses: Detailed examination of tiny structures,
studying individual cells, bacteria, or small particles.
High-power objective lens (MPO)
A smaller lens with a higher magnification power.
* Higher numerical aperture (NA) for better resolution
and clarity.
* Requires careful focusing and handling due to higher
magnification.
High-power objective lens (MPO)
Magnification Range: 100x more
Oil-Immersion Objective objective lens (OIO)
Typical Uses: Examining microscopic structures and observing
details at the cellular level.
Oil-Immersion Objective objective lens (OIO)
Characteristics and specifications
* It is used with a unique immersion oil to improve
resolution by reducing light refraction.
* Highest magnification power among the objective
lenses.
- Requires a drop of oil to be placed on the specimen
for optimal performance.
Oil-Immersion Objective objective lens (OIO)
This knob is rotated until an
image appears. It is used for appropriate focusing. This
could remove the body tube or the stage up or down,
depending on the model of the microscope.
Coarse adjustment knob.
this smaller knob is rotated
until the image becomes clear and well-defined. It is
used for fine focusing.
Fine adjustment knob.
. This is held by the right hand, if the microscope is
being carried. The left hand should support the base.
Armq
This is the platform where the microscopic slide or
specimen is placed.
Stage.
are used to secure the slide
or specimen in older models.
Stage clips
performs the same functions, which only
requires rotation of two screws to move the slide forward,
backward, or sideward.
mechanical stage
is the part of the substage
composed of a system of metal blades which can be
manipulated, using a lever.
Iris diaphragm.
This is the part of the substage
composed of a system of lenses is used to concentrate
light.
Abbe condenser
The
plane surface is used with daylight illumination and the
concave surface is used with artificial illumination.
Mirror.
This part is used to tilt the
microscope.
Inclination joint.
This is a short part connected to the
base and upper parts of the microscope. It is
used to support the upper parts of the
microscope.
PillaR
This is the U, Y or V-shaped metal part
supporting the whole microscope.
Base
