Microscope Quiz Flashcards
What are cover slips and immersion oil designed with and what are they for?
refractive indices to trap more orders of light i.e. give more light and better image quality
Define resolution.
the smallest distance between 2 points on a specimen that can be distinguished as 2 separate entities
What part of the microscope is the resolution dependent on?
the numerical aperture and the substage condenser
What does a higher numerical aperture allow for?
more orders of light entering objective therefore getting a higher resolution
What areas of the microscope are important for proper use?
eyepiece, objective, stage, condenser, aperture diaphragm, and field diaphragm
What areas of the microscope are important for cleaning?
eyepiece, objective, stage, and condenser
As the lens size gets smaller, what happens to the magnification?
it rises and less light comes through
What is the purpose of the condenser diaphragm?
it opens and closes to regulate light coming from the field diaphragm
When the aperture diaphragm is closed what happens to the contrast, resolution, light, and focal depth?
contrast is increased, there is a loss of detail in resolution, illumination is decreased, and there is a deeper focal depth
What is contrast?
the difference in light intensity between the image and the adjacent background relative to the overall background
How can you increase contrast on a microscope?
by closing the aperture diaphragm or lowering the condenser stage
What is contrast and glare good for identifying?
foreign bodies, fungal elements, eosinophil granules, crystals, and ectoparasites
When initially using the microscope, what should the light be set at?
50%
What are the steps of Kohler illumination?
- focus on slide using 10x objective
- lower the condenser stage
- close field diaphragm and aperture diaphragm
- slowly raise condenser stage until a small circle of light is seen (raise until edges are sharp, center objective view using set screws)
- open field diaphragm until light fills objective view
- adjust aperture diaphragm for objective na
When examining mites, what is the best thing to do to increase refractivity?
move the condenser stage down
When examining stained bacteria, what is the best thing to do to decrease refractivity?
move the condenser stage up
What should you do on the microscope to visualize eosinophils, parasites, and other organisms?
close the aperture diaphragm
When should you always use a cover slip?
when examining a slide with 40x objective
What should you use to clean the lens of a microscope?
Q-tips
What should you use to clean the eyepieces?
kim wipes, lens paper, or cotton tipped applicator
How should you clean the objectives?
use lens cleaner and lens paper or cotton-tipped applicator
clean surface by rubbing with a circular motion
How should you clean the microscope stage?
use general cleanser or lens cleanser and make sure to clean under the slide holder
What is the best way to clean the condenser?
remove it from the body of scope and then use a cotton-tipped applicator or kimwipes to clean it
How do you clean the field diaphragm?
use a cotton-tipped applicator or kimwipe