Histology Intro

Question 1

Requirements to use light microscopy

Answer 1

1. Preserved in a life-like state
2. Transparent so light can pass
3. Thin and flat
4. Components should be differentially stained so they can be distinguished.

Question 2

Histology

Answer 2

Study of tissue structures and how they are arranged

Microscopic anatomy linked to function

Question 3

Ways of preparation

Answer 3

Whole mount

Squash

Smear

Section

Question 4

Steps of slide preparation

Answer 4

Fixation: Immobilize
⬇︎
Embedding: Solidify
⬇︎
Sectioning: Thin slices
⬇︎
Staining: Visibility

Question 5

How do we study organs with microscopy?

Answer 5

Study many sections in different planes (serial selections)

Question 6

Planes of section

Answer 6

Longitudinal

Transverse/cross

Oblique

Question 7

Artifact

Answer 7

Structure that is not normally present in living tissues

Caused by errors in slide prep (fixation, etc.) or surgical removal.

Question 8

Basophilic Stains

Answer 8

Dye reacts with acidic (anionic) components of cells

Nucleic acids (acidic) bind to basic dye

Nuclei, RER, DNA, RNA, extracellular matrix, blue to purple.

Question 9

Acidophilic stains

Answer 9

Dyes react with basic (cationic) components of cells

Proteins (cytoplasm) are basic and bind to acidic dye

Cytoplasm, collagen, mitochondria, lysosome, muscle, colloid, red blood cells,

Pink to orange

Question 10

Basic tissues

Answer 10

Epithelial

Connective

Muscle

Nervous