Equine reproduction Flashcards
How long is the mares normal oestrous cycle?
21 days
How long is the mares normal oestrus?
2-7 days
What are counted as high risk mares?
Previously positive mares
Travelling outside UK
What do you swab horses for?
Contagious equine metritis - notifiable
Pseudomonas
Klebsiella
What causes contagious equine metritis?
Taylorella equigenitalis
What is a sign of active CEM infection?
Purulent discharge
What are the two types of culture used for the swabs?
Aerobic and microaerophilic
What are the two notifiable diseases that are detected by serology?
Equine viral arteritis
Equine infectious anaemia
What does a seropositive infection mean for equine infectious anaemia?
Definite infection
Unlike equine viral arteritis which has a vaccination
What is the ideal perineal conformation?
Vulva 0-10 degrees from vertical
3/4 of the vulva below the pelvic brim - top open
Mostly vertical
Have a vulval seal
What are the steps of a breeding soundness exam?
Prebreeding swabs General clinical exam Repro clinical exam - Check perineal conformation Rectal ovarian palpation and US Check vestibule, vagina and cervix Endometrial swabs
What are the different breeding methods in mares?
Natural covering
Fresh, chilled and frozen AI
Deep intrauterine insemination
When is deep intrauterine insemination used?
When mares react badly to AI and get endometriosis
How do you predict ovulation?
Follicle - 4-5cm
Oedema pattern on ultrasound
Flattening/softening of cervix
How to bring forward the spring transition into oestrous?
Administer progestagens and then withdraw them - altrenogest
How do you shorten the luteal cycle?
Use synthetic progesterone - estrumate
What is required to be able to shorten the luteal phase of the oestrus cycle?
Need a mature CL
How do you prevent oestrus?
Oral altrenogest
GnRH vaccine
How do you treat endometritis?
Flush sterile saline and oxytocin/prostaglandins
What causes infectious endometritis?
Commensals - common
Taylorella/klebsiella/pseudomonas
Yeast
Fungi
What are the main causes of poor fertility in horses?
Erratic oestrus
Persistent CL
Anovulatory follicle
Granulosa cell tumour
What electrolytes increase before foaling?
Calcium
Potassium
What is the rule of 3 when foaling?
Stand by 1, suckle by 2, pass placenta by 3
What events occur post partum?
Uterine involution
Foal heat - 5-9 days
How do you induce parturition?
Low dose oxytoxin
What is red bag delivery?
Allantochorion is passed before the foal is delivered - premature placental separation
When does it count as dystocia?
When the allantochorion ruptures but
No foal/amnion at vulva in 5 mins
No strong contractions at 10 mins
When should you stop vaginal assisted delivery?
When there has been no progress in 5 mins
What does clenbuterol do?
Stops uterine contractions
How do you treat retained foetal membranes?
Oxytocin
Manual traction
What can retained foetal membranes cause?
Endometritis
Laminitis
Shock
Death
What is a first degree perineal laceration?
Just the vulval lips
What is a second degree perineal laceration?
Vulval lips and muscle layers
What is a third degree perineal laceration?
Common opening between vulva and anus
Complete disruption of rectovestibular shelf
When should you inspect for cervical trauma?
Dioestrus
What can post partum colic mean?
Uterine haematoma
Hypocalcaemia
What are the symptoms of hypocalcaemia?
Dry faeces
Spontaneous diaphragmatic flutter
Tetanic spasms
What is an issue with premature lactation?
Potential for failure of passive transfer to foal
collect colostrum
Check for placentitis
How can you diagnose pregnancy in a mare before 21 days?
Transrectal ultrasound - from d12
Elevated plasma progesterone
Failure to return to oestrus
When can you perform transrectal palpation to diagnose pregnancy?
From 21 days
What can you measure in a blood test to diagnose pregnancy in a mare from 60 days?
Plasma equine chorionic gonadotrophin
When does the foetus go out of reach?
85 days
What is the difference between a pregnancy and a cyst?
Pregnancy is spherical, specular reflections
Cyst is irregular
How do you check for twins?
Check for 1 or 2 CL
Can also US or manually palpate
How are twins managed in the mare?
Manual rupture - pinch
(Separate if not implanted to prevent killing both
Or abort both with PG
What are the risks with pregnancy diagnosis?
Rectal tears
Risk of killing both
Abortion
How can you tell if there is a rectal tear?
Blood on rectal glove
Sudden loss of rectal tone
What are the risk factors for rectal tears?
Rectal examination
Dystocia
Age
Breed
How can you reduce the risk of rectal tears?
Sedation, relaxants - buscopan
What should you do if there is a rectal tear?
Admit to owner immediately
ABs, NSAIDs
Absorbent packing
Referral
What produces progesterone in horses?
Prim/secondary CLs initially
Then supported by equine chorionic gonadotrophin from endometrial cups
Up until what point can you do intended abortion by vet?
<35 days - until endometrial cups form
How does oestrus next present after embryo loss at 5-15 days?
Early return to oestrus
How does oestrus next present after embryo loss at 16-36 days?
6 weeks after luteolysis of primary CL
How does oestrus next present after embryo loss at 36-140 days?
5 months after luteolysis of secondary CL
What are the 3 main infectious causes of abortion in mares?
Bacterial placentitis
Equine herpes virus
Equine viral arteritis
What are the clinical signs of bacterial placentitis?
Vulval discharge
Premature lactation
Abortion
HOw do you diagnose bacterial placentitis?
Measure thickness of uterus and placenta
Swab cervix
What is the main pathogen causing bacterial placentitis?
Strep zooepidemicus
Which type of equine herpes virus is most likely to cause abortion?
EHV-1
What is important in the treatment of EHV-1?
Aborted foetus is highly contagious to other mares so dispose correctly
What are the clinical signs of Equine viral arteritis?
Abortion
Conjunctivitis
Scrotal swelling
What are some non infectious causes of pregnancy loss?
Twisted umbilical cord
Uterine torsion
Twins
Premature placental separation
How do you treat uterine dorsoretroflexion?
Clenbuterol and gentle exercise
What are the signs of a ruptured prepubic tendon?
Massive ventral swelling
Pitting oedema
What fluid is involved in hydrops uteri?
Allantoic fluid
How do you treat hydrops uteri?
Abortion
Treat for shock after fluid loss
How can you clear any post covering luminal fluid?
Oxytocin
Prostaglandins
What method of breeding do all thoroughbreds use?
In hand breeding
What is the maximum no of covers?
3 a day
What are the 5 stages of a stallion breeding soundness exam?
General health - vaccination status Gross examination Bacteriological screening Virology Semen elavuation
What are the 3 main stallion vaccinations?
Influenza
Tetanus
EHV-1 and EHV-4
Where should you do the bacteriological swabs?
Urethral orifice
Urethral fossa
Prepuce
Pre-ejaculatory fluid
What/how many bacteriological swabs should a low risk stallion have?
2 negative sets of swabs >7 days apart
What pathogens are bacteriological swabs screening for?
CEMO
Klebsiella
Pseudomonas
How do you treat bacterial venereal pathogens?
Topical - clean penis
Inoculate with health bacteria broth from normal stallion
How do you treat equine viral arteritis?
Symptomatic treatment - spontaneous recovery over 1 month
30% shed in semen for life - castrate these
What is required after semen collection?
Removal of the gel fraction
Why remove the gel fraction?
Reduces sperm motility
Reduces longevity
How do you remove the gel fraction?
Filtration at entry to collection vessel
Suction with pipette
What is the percentage of progressive motility of normal sperm?
60-80%
What is the percentage of morphologically live normal sperm?
60%
What is the normal volume of stallion ejaculate?
15-100ml
What stain do you use to evaluate semen morphology?
Nigrosin
Eosin
What different semen extenders are there?
Milk or egg based
What ratio of semen extender do you use?
1:3 - semen:extender
How do you prepare frozen semen?
Centrifuge
Dilute into cryoprotective solution
Cool to 5 degrees
Freeze in liquid nitrogen
What is semen stored in?
0.5ml straws
What is the recommended minimum sperm per dose?
200 million progressively motile sperm per dose
What is the BEVA minimum standard for fresh semen?
Progressively motile with normal morphology >50%
What is the BEVA minimum standard for chilled semen?
> 500x10^6 progressively motile sperm/dose at AI
What is the BEVA minimum standard for frozen semen?
> 30% PM sperm
How do you unfreeze frozen semen?
Fast thaw for 30 secs at 37 degrees
What can temporarily reduce spermatogenesis for a few months?
A fever
How long does spermatogenesis take to occur?
57 days
What doe abnormal testes look like on ultrasound?
Generalised/focal change to testicular echotexture - cellular infiltration, cysts etc.
What can cause haemospermia?
Urethritis
Penile laceration
Accessory gland infection
What can cause urospermia?
Cystitis - bladder infection
Cauda equine syndrome
What can cause pyospermia?
Seminal vesiculitis - bacteria
Infection of accessory glands
When would you do a urethroscopy?
Visualise uroliths
Identify source of blood/pus
What can cause ejaculatory dysfunction?
Neurological
Vascular
Pain - orthopaedic
What is the goal of a caslicks procedure?
Improve the vulval seal
Reduce risk of endometritis/ascending placentitis
What is another word ofr a caslicks procedure?
Episioplasty
When are caslicks done?
When there is poor conformation
Previous foaling trauma
Pneumo/faeco/urovagina
When is an episiotomy/removing caslicks done?
2 weeks before foaling date
When does urovagina usually occur?
Thin multiparous mare
Sloping vaginal floor
What is a uteropexy?
Elevate uterus by fixing uterine horns to dorsolateral abdominal walls
Fluid drains out
When is a caesarean performed?
Dystocia
Uterine tear
Uterine torsion
Elective
What are the complications of dystocia?
Repro tract trauma
Retained foetal membranes
Metritis
Bladder prolapse
What is a rectovaginal fistula?
Tear on inside but not all the way to the outside, between the anus and the vulva
Anal sphincter intact
How do you treat a 1st or 2nd degree perineal lacteration?
Repair when fresh like a caslicks
How do you treat a 3rd degree perineal laceration?
Leave time to granulate Clean daily Surgically close rectal mucosa close vaginal mucosa Close skin between anus and vulva
When should you examine the cervix for laceration?
In all dystocia cases at 3 weeks post partum
What should you do if there is a uterine laceration?
C section
May repair laceration before delivering foal
When should you perform an ovariectomy?
Behaviour changes
Large ovary
What is a common cause of large ovary or behaviour changes in a mare?
Granulosa cell tumour
What behaviour changes can be seen due to a granulosa (theca) cell tumour?
Persistent oestrus behaviour
Nymphomania
Aggressive - stallion tendencies
What is seen on serology that suggests a granulosa cell tumour?
Antimullarian hormone
Why are horses castrated?
Behaviour modification
Improve gene pool
Medical reasions
When does castration occur?
Between 6 and 24 months
When both testicles are present
Why does castration occur at this age?
Allow handling training
Allow conformation of entire - muscles
After racing as colt
When should open castration not be performed?
In mature stallions
Donkeys/mules
What is semi-closed castration?
Vaginal tunic incised, contents checked then closed
What type of castration must be done under general anaesthetic?
Semi-closed
Closed
What is closed castration?
Testicle is removed in the vaginal tunic which is ligated and removed
What can be used in standing field castration?
Emasculators
How should emasculators be used?
Nut to nut
Leave for 3-5 mins when on
What are some castration complications?
Intestinal herneation Persistent arterial haemorrhage Scrotal/preputial oedema Infected tissue Inflamed prepuce with discharge
What is the name fore a cryptorchid horse?
Rig
What are the different locations of a retained testicle?
Abdominal or inguinal
How is cryptorchidism diagnosed?
With a history
Palpation - sedate
Hormone assay
How can you palpate for a testicle in the internal inguinal ring?
Transrectally
What hormone is looked for on a hormone assay to identify cryptorchidism?
Anti-mullarian hormone
How can cryptorchid horses be treated
Laparoscopic surgery - standing sedation or GA
What are the two different methods of GA cryptorchid surgery?
Midline or inguinal approach
What is phimosis?
Inability to protrude penis from prepuce
What is paraphimosis?
Inability retract penis into prepuce
What is priapism?
Persistent erection in the absence of stimulation
What neoplasia of the penis may metastasise and where to?
Squamous cell carcinoma
Melanoma
What neoplasia of the penis is a space occupying lesion that rarely metastasises?
Sarcoid
What are some complications of penile haematoma?
Urethral obstruction - catheterise
Paraphimosis/phimosis
What can cause paraphimosis?
Oedema
Damage to innervation
ACP tranquilisers
What can prolonged paraphimosis cause?
Oedema and swelling
Stretched nerves
Stretched retractor penis muscle
How do you treat/manage paraphimosis?
Retain penis in sheath with purse string suture
Support in sling
What is the most common cause of priapism?
ACP (phenothiazine)
How can you treat priapism?
Adrenaline into corpus cavernosum - contracts smooth muscle
What is segmental posthectomy (reefing)?
Take a circumferential cuff of the epithelium from the internal preputial lamina to help maintain the position of the penis in the prepuce
What is penile amputation called?
Phallectomy
What can phimosis cause?
Urine scalding within the sheath
When is phimosis normal?
In foals under 1 month
What are the two different types of strictures that can cause phimosis?
Constriction of the preputial orifice
Constriction of the preputial ring
How is constriction of the preputial ring treated?
Reefing
What are uroliths made up of?
Calcium carbonate
How are uroliths treated?
Perineal urethrotomy - crush stone and flush