DNA Structure and Replication (KING)

Question 1

Cell cycle protein that is involved in DNA damage-mediated cell cycle arrest

Answer 1

p53

Question 2

Failure to thrive in neonatal period accompanied by muscular hypotonia

loss of a group of PATERNALLY inherited genes d.t. DELETION OF REGION OF CHROMOSOME 15

Answer 2

PRADER WILLI SYNDROME

• short stature
• hypotonia
• small hands and feet
• obesity
• mild to moderate retardation

Question 3

Camptothecins are an alkaloid class if anticancer drugs used in treatment of colon and breast cancers.

These compounds function by (-) which of the ff enzymes?

Answer 3

TOPOISOMERASE I - induces single strand breaks in DNA during replication

Irinotecan
Topotecan

Question 4

Mechanism of Action of Taxol (Paclitaxel)

Answer 4

Binds to microtubules which stabilizes them preventing their shortening and interfering with cell division

Question 5

MOA of Doxorubicin

Answer 5

(-) topoisomerase II

Question 6

Composition of eukaryotic nucleosome core

Answer 6

2 copies each of histone H2A, H2B, H3 and H4

Question 7

(-) the actions of topoisomerase II

Answer 7

ANTHRACYCLINS

Doxorubicin
Daunorubicin

Question 8

Involved in the regulation or progression through the G1 phase of the cell cycle

Answer 8

cyclin D - CDK4/6

pRB - MAJOR TARGT

Question 9

Activates the expression of cyclin A, cyclin E and CDK2

Answer 9

E2F

Question 10

Ubiquitin ligase that controls the levels of M phase cyclins and other regulators of mitosis

Answer 10

ANAPHASE PROMOTING COMPLEX (APC)

Question 11

Causes mutation by the process of dimerization of adjacent pyrimidines

Answer 11

ULTRAVIOLET LIGHT

Question 12

Major role of 3’ to 5’ exonuclease activity of DNA polymerases

Answer 12

removes mismatched nucleotides

Question 13

The final step in the maturation of the newly synthesized lagging strand of DNA is formation of PDE bonds b/w the Okazaki fragments. This is catalyzed by which enzyme?

Answer 13

LIGASE