DNA Replication

Question 1

DNA REPLICATION

Answer 1

semiconservative

continuous and discontinuous (Okazaki fragment)

5’ to 3’ direction

Question 2

Particular consensus sequence in genome where DNA replication begins

Answer 2

ORIGIN OF REPLICATION

AT rich sequences (TATA box) - found in promotes and origins of replication

Question 3

Y shaped region along DNA template where leading and lagging strands are synthesized

Answer 3

REPLICATION FORM

Question 4

UNWINDS DNA template at replication fork

Answer 4

HELICASE

deficient in BLOOM SYNDROME

Question 5

Prevent strands from reannealing

Answer 5

SINGLE STRANDED BINDING PROTEINS

Question 6

Create a single or double stranded break in the helix to ADD OR REMOVE SUPERCOILS

Answer 6

DNA TOPOISOMERASE

EUKARYOTES:
IRINOTECAN/TOPOTECAN - (-) TOP I
ETOPOSIDE/TENIPOSIDE - (-) TOP II

PROKARYOTES:
FLUOROQUINOLONES - (-) TOP II (gyrase) and TOP IV

Question 7

Make an RNA primer on which DNA polymerase III can initiate replication

Answer 7

PRIMASE

Question 8

PROKARYOTES ONLY

Elongates leading strand by adding deoxynucleotides to the 3’end

Answer 8

DNA POLYMERASE III

Question 9

PROKARYOTES ONLY

Degrades RNA primer replaces it with DNA

Answer 9

DNA POLYMERASE I

Question 10

Catalyzes the formation of a phosphodiester bond within a strand of double stranded DNA

JOINS OKAZAKI FRAGMENT

Answer 10

DNA LIGASE

links DNA

Question 11

EUKARYOTES ONLY

A reverse transcriptase (RNA dependent DNA polymerase) that ADDS DNA ( TTAGGG) to 3’ ends of chromosomes to avoid loss of genetic material with every duplication

Answer 11

TELOMERASE