Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Question 1

Major pathway for FORMATION OF ATP

Also provides substrates for gluconeogenesis, AA synthesis and FA synthesis

Answer 1

Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Question 2

Site of Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Answer 2

In all cells with mitochondria

MITOCHONDRIAL MATRIX
EXCEPT - succinate dehydrogenase (inner mitochondrial membrane)

Question 3

Substrate of Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Answer 3

Acetyl CoA

Question 4

Products of Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Answer 4

2 CO2
1 GTP
3 NADH
1 FADH

Question 5

Rate Limiting Step of Citric Acid Cycle/Krebs Cycle/ Tricarboxylic Acid Cycle (TCA)

Answer 5

Isocitrate –> alpha ketoglutarate

ISOCITRATE DEHYDROGENASE

Question 6

acetyl CoA + Oxaloacetate –> citrate

Answer 6

CITRATE SYNTHASE

Question 7

Citrate –> Isocitrate

Answer 7

Aconitase

(-) by fluoroacetate

Question 8

Isocitrate –> alpha ketoglutarate

Answer 8

Isocitrate Dehydrogenase

produces CO2 and NADH

Question 9

alpha ketoglutarate –> succinyl CoA

Answer 9

alpha ketoglutarate dehydrogenase

produces CO2 and NADH

(-) by ARSENITE
increased by ammonia

Question 10

succinyl CoA –> succinate

Answer 10

Succinate Thiokinase

produces GTP by substrate level phosphorylation

Question 11

succinate –> fumarate

Answer 11

Succinate Dehydrogenase

produces FADH

(-) by Malonate

Question 12

Fumarate –> Malate

Answer 12

Fumarase

Question 13

Malate –> Oxaloacetate

Answer 13

Malate Dehydrogenase

produces NADH

Question 14

To replenish compounds in TCA cycle filling reactions takes place. These filling reactions are called

Answer 14

Anaplerotic Reactions

PYRUVATE CARBOXYLASE - major filling up reaction
Transaminase
PEP Carboxykinase