CHapter 6- Microbial Metabolism Flashcards
Microbial cells must always do 2 things
synthesize new parts (cell walls , ribosomes, nucleic acids) and harvest/convert energy to power reactions
sum total of all enzymatic chemical reactions in cell/ living organisms to maintain life
metabolism
Synthesizing new parts & harvesting energy to power reactions
metabolism, cells must do this
process that degrades compounds (glucose) to release energy; cell use the energy to synthesize ATP
Catabolism
harvest the energy released during the breakdown of compounds and use it to make ATP
Catabolism
What is produced from catabolic processes?
Precursor metabolites/ waste products (acids, carbon dioxide)
compounds produced during catabolism that can either be further degraded or used in anabolism to make subunits of macromolecules
precursor metabolites
chemical reactions that synthesize and assemble subunits of macromolecules, using ATP for energy
anabolism/biosynthesis
________ processes produce ATP , _______ use ATP
catabolic ; anabolic
capacity to do work;
energy
kinetic energy
moving energy; energy of motion
potential energy
stored energy
photosynthetic organisms harvest energy from?
sunlight
obtain energy from organic compounds
Chemoorganotrophs
energy available to do work; energy released when bond is broken
free energy
reaction that releases energy bc reactants have more free energy than products
exergonic reactions
reaction that requires energy bc products have more free energy than reactants
endergonic reactions
energy released from ______ reactions drives _________ reaction. energy released from ________ processes drives _________ processes.
exergonic/endergonic; catabolic/ anabolic
series of chemical reactions that converts starting compounds to end products
metabolic pathways (can be linear, branched,
why are enzymes important to metabolic pathways?
Without enzymes, energy yielding reactions would occur too slowly
Role of enzymes in reactions
enzymes speed up conversion of substrates into product by lowering activation energy
substance which enzymes acts/performs on to perform products
substrates
initial energy required to break chemical bond
activation energy
biological catalysts that increase rate of reaction by lowering activation energy
enzymes
enzyme characteristics
highly specific for substrates, cannot be changed by reactions and are reusable
site on enzyme where substrate binds by weak forces;
active site
binding of substrate to active site that causes slight change of enzyme shape so bonds get destabilized and new ones can form
enzyme substrate complex
assistance of an attached non protein component required by some enzymes
cofactor (Mg, Zi, Cu + trace elements)
assistance of a loosely bound non protein ORGANIC compound that help enzymes transfer molecules/electrons; derived from vitamins
coenzymes (FAD, NAD+, NADP+)
energy currency of cell
ATP
How do cells use energy to make ATP?
by adding and inorganic phosphate to ADP to yield ATP
How do cells release energy from ATP?
from removing inorganic phosphate from ATP to yield ADP
Processes chemoorganotrophs use to make ATP
substrate-level phosphorylation and oxidative phosphorylation
chemical addition of a phosphoryl group to an organic compound
phosphorylation