Chap 1 Flashcards
Unit of DNA that encodes for something
gene
Characteristics of Micro organisms
SMALL, typically unicellular; possible live in colony w/ other cellular organisms
Cell theory
all livings things are composed of one or more cells, cells are basic unit of life, and living things arise from pre-existing cells
Biogenesis
production of living things from other living things; opposes spontaneous generation
Process where high heat is applied for short period of time, concept generated by Louis Pasteur
Pasteurization
Scientific method
process of formulating a testable explanation for a question, experimenting the hypothesis & drawing conclusions based on the results
Normal Microbiome
group of microoragnisms that colonize body surfaces & usually dont cause disease
Microbiome
community of microoragnisms & their genetic info in a given environment
A disease causing microbe
Pathogen
Emerging infectious diseases
Disease that is increasing in incidence/ geographic range
Category of related organisms; 1st part of scientific name
Genus
Group of closely related strains; basic unit of taxonomy; italicized
Species
3 Domains of Living Organisms
Bacteria, Archeae, Eukarya
Bacteria classified as
single cell prokaryotes w/ peptidoglycan in their cell walls
Main types of bacteria
Cocci & rods
Other shapes of bacteria
Spirilla, Vibrios, Spirochets
Archea classified as
single cell prokaryotes that look like bacteria, but have no peptidoclycan; grow in extreme environments
Eukarya classified as
eurkaryotes
Eukaryote examples include
Fungi, Algae, Protozoa
Contain chitin in their cell wall & use organic compounds as food; range in sizes from yeast to multicellular molds
Fungi
Have simple reproductive structures & no organized vascular system; Use sunlight as energy source
Algae
Motile, single cell organism that also use organic compounds as food
Protozoa
Acelluar Infectious Agents include (nonliving microbes w/ no cells)
Viruses, Viroids, Prions
Acellular microbe that consists of either RNA or DNA . has protein coat, infect & stay in host cells, and use their machinery to replicate
Virus
Consists of single RNA, no protein coat, infects hosts cell & use their machinery to replicate; usually cause plant disease
Viroids
Consists of ONLY protein; no DNA, no RNA that causes misfolded versions of normal cellular protein (usually found in brain)
Prions
“patho”
DISEASE
“encephalo”
BRAIN
How do we classify/ identify minute differences in a species ?
Grouping them into strains
Prokaryotes DO NOT “before nuts”
have membrane bound nucleus
Eurkaryotes HAVE “true nuts”
Membrane bound nucleus & organelles
Examples of Micro organisms
Bacteria, Archaea, Fungi, Algae, Protozoa, Multicellular Animal Parasites
Who made the first observations that living things were composed of cells?
Robert Hooke, 1600s
Arguments against spontaneous regeneration like Cell theory/ Biogenesis was discovered & introduced by
R. Virchow, 1800s
Cell Theory also represents
Biogenesis
Who was responsible for the first observation of microbes by studying rain water and kicking off the community’s interest in cell theory?
Anton van Leeuwenhoek
Belief that organism can arise from non living matter, believed until mid 19th century
Spontaneous Generation
Experiment with meat and jars that showed cloth prevented flies from laying eggs on meat, but not how microbes could still exist/enter the jar & did not convince the community to change spontaneous generation beliefs
Franciso Redi
This scientist supported spontaneous generation and his experiments assumed boiling broth kills any living thing inside, and if it appeared, must be bc of spontaneous generation
John Needham
This scientist opposed Needham’s by repeating his experiment, BOILED LONGER AND SEALED FLASKS, so life’s vital forces would not get in. However, ppl believe the heat killed vital forces so it was not accepted
Father Spallanzani
Louis Pasteur’s swan neck flask experiment concluded
microbes already exist in air, as his flasks kept microbes out, but let the air in
Louis Pasteur exposed the microbes can both
be present in non living matter (solid, liquids and air) and killed by heat
Aseptic techniques prevents
contamination of unwanted microbes
Key DISCOVERIES in Golden Age of Microbiology
Vaccination, Germ Theory , Pasteurization and Fermentation
Conversion of sugar to alcohol to make beer and wine, process originated by
Fermentation, Louis Pasteur
Louis Pasteur developed microbial techniques for
PRESERVING FOOD and LIQUID
Theory that microbes/ microoraganisms can cause disease
Germ Theory of Disease
The idea that microorganisms may cause disease
Germ Theory of Disease
This scientists showed silkworm disease was caused by fungus
Agnostio Bassi, 1800s
Dr who promoted hand washing to prevent fever amongst OB patients (pioneer of antiseptic procedure)
Dr. Ignaz Semmelweis
Dr who used chemical disinfectant like carbolic acid to prevent surgical wound infections
Joseph Lister
Proved bacteria caused negative effects of anthrax
R. Koch
Purpose of Koch’s postulates
Prove specific microbes causes specific disease
This person found a way to protect ppl from smallpox (essentially created start of vaccines)
Edward Jenner 1700s
Protection is aka
IMMUNITY/ VACCINATION
Benefits of Microorganisms include
Nitrogen fixation, oxygen production, breakdown of materials (sewage, cellulose and wastewater)
Example of micro organisms in food production
Baking bread using yeast, fermentation to make alcohol or dairy
Examples of micro organisms in genetic engineering
intro of genes to another organism, diseases resistant plants, production of medications (insulin/diabetes)
Role of normal human microbiome
prevent diseases by competing with harmful microbes, help digestion, promote immune systems, can decrease allergies, affect brain chemistry and weight
Vaccines have nearly eradicated
smallpox, plague death, polio
Example of infectious disease in non human population
Irish Potato famine, english foot and mouth disease, wheat blast
Emerging Infectious Disease Example
Ebola, Zika, FLu, Lyme, AIDS, Mad Cow, Malaria, Tuberculosis
All Living Things Classified in 3 Domains
Bacteria, Archaea, Eukarya
Who DOES NOT have peptidoglycan got in their cell wall?
Archaea and Eukarya
Who Has membrane bound organelles?
Eukarya
Who DOES NOT have membrane bound organelles?
Bacteria and Archaea
What comes first, capitalized & Italizicized?
Genus
WHat comes after genus, lower cases, and italicized?
Species
Binomial Nomenclature to identify organisms composed of
Genus & Species
groups of species that are genetically related but differ in some way
Genus
Specific name of organism that differentiates it from others in genus
Species
Classification of living things to establish relationships of orgranism in one group to another
Taxonomy
Prokaryotes are known as ________ because members of same species are genetically all identical to one another
clones
Population of cells that have derived from single parent
Clones
Identical cells are considered what kind of culture?
Pure culture
Groups of species that are genetically related but differ in some way identified by #s, letters or names followed by species name __________
Strains
Microbial Nomenclature Order
Genus. Species. Strain
Bacteria Characteristics
Single Celled, prokaryotes, most have specific shapes, most use flagella , multiple by binary fission, have wide energy source, cells of same species usually one shape
How do prokaryotes multiply?
Binary Fission
Bacteria Shapes?
Coccus, Rods (Bacillus), Vibrio, Spirillum, Spriochette, Pleomorphic
Arachaea Characteristics
Single celled prokaryotes, cell wall has NO peptidoglycan, many exist/ have extreme environments
Eukarya Characteristics
have nucleus, membrane bound organelles, more complex, members are fungi , algae, protozaoa
Parasitic worm, type of eurkarya
Helminth
Groups of differences in a pure culture
Strain
Microbial World Relation Tree
Microbial Nomenclature Example
Bacterial shape?
Coccus (C for Circle)
Bacterial Shape?
Rods (Bacillus)
Bacterial Shape?
Vibrio
Bacterial Shape
Spirillum
Bacterial Shape?
Spirochette
Prokaryotes do not have a ________ but instead have a ________ where genetic material is kept.
membrane brand nucleus, nucleoid