Chapter 3- Staining

Question 1

How does capsule staining work?

Answer 1

Common dyes stain capsules poorly, instead background is stained to make capsule visible against dark background

Question 2

simple staining - basic dye carries positive charge

Answer 2

technique that uses basic dye to add color to cells

Question 3

Acidic dyes cannot stain cells, therefore used for_____

Answer 3

negative staining (which colors background)

Question 4

staining technique that uses acidic dye to stain background so colorless cells can be seen

Answer 4

Negative staining

Question 5

technique used to differentiate types of bacteria

Answer 5

Differential staining

Question 6

Differential staining types

Answer 6

Acid Fast and Gram Stain

Question 7

Gram positive cells stain _____

Answer 7

purple (cell wall prevents dye from being washed out during decolorization)

Question 8

gram negative cells stain_____

Answer 8

pink (thinner layer of peptidoglycan damaged by decolorizing agent and cannot hold back dye complex)

Question 9

Gram Staining Process

Answer 9

Question 10

procedure used to stain microorganisms that do not readily take up dyes

Answer 10

acid fast staining (these microorganism contain mycolic acid cell walls that have wax like structure. therefore harsh methods needed)

Question 11

Different Stain Types

Answer 11