Chapter 27 - Management of Superficial Wounds, deeps and chronic wounds, sinus tracts, fistulas Flashcards
What is the largest organ in the body
skin
What are the main functions of the skin
- Protect against wear + baterial invasion
- Aid thermal regulation
- Prevent water loss
What is the average of the skin thickness?
3.8 mm thcik
near body opening 3.3 mm
mane and tail 6.2 mm
The skin of horses is _______ than swine, goats, and sheep and thinner than that of ________
The skin of horses is __thicker_____ than swine, goats, and sheep and thinner than that of _____beef catle___
From superificial to deep the skin is composed by epidermis and dermis. Mention the 4 layers of keratinocytes of epidermis from superficial to deep
- Stratum corneum: diminish water loss
- Stratum germinativum
- Stratum spinosum
- Stratum basale
What is the main vascular supply of the dermis?
it comes from the subcutaneous area
The dermis underlis and supports the epidermis. Mention the 2 layers
- Superficial papillary layer
- Deep reticular layer
What other ç beside keratinocytes are visible in the epidermis?
- melanocytes
- Merkel cells for mechanoreception of light touch
- Langerhans ç - engulfing foreign material
Where does the dermis has a third layer of collagenous fibers?
In the:
1. lumbar
2. sacral
3. gluteal regions
To each hair follicle the are _____(nº) sebaceous glands, sweat glands and nerves
Ckevage lines or _________ (1w) lines of tension whose orientation is parallel to the predominant orientation fo the collagen fibers
Langer lines. When possible incisions should be made parallel to the clevage lines
What is the most important step in wound therapy?
Wound assessment
What should be assessed before using sedation?
Systemic status
What could sedation cause in a horse that has lost a lot of blood?
Collapse
What alternatives exist to systemic sedation?
Manual restraint, local anesthesia
What is applied over the wound before clipping hair?
Sterile lubricating gel
What can make an initially insignificant wound dangerous later?
Invaded synovial structures
What does débridement reduce in a wound?
Bacterial load, necrotic tissue
What factor reduces the number of bacteria required for infection in the presence of foreign material?
10-fold (from 10^5 to 10^4)
What are the most common types of débridement?
Sharp, mechanical, chemical, biological, autolytic
What types of débridement are preferred in equine wound care?
Sharp, autolytic
What is the major drawback of sharp débridement?
Irreversible tissue removal
What are tools used for sharp débridement?
Scalpel, scissors, lasers
What can mechanical débridement be performed with?
Woven gauze,
lavage,
dressings
What is the recommended pressure for wound lavage?
10-15 psi
19 G needle or catheter to a 35mL sering the psi is how much?
15 psi =perfect
Which fluids are often used in wound lavage?
Dilute antiseptics, saline
Why tap water should be avoided?
because is cytotoxic to fibroblasst
type of debridement
- Mechanical débridement with sterile saline.
Type of gauze a) and B
Nonwoven (a) and woven (b) gauze.
how many psi?
16 Gneedle to punch 4-8 hores in cap of a 1L bottle –> only 3.9 psi
Mention the different solution for chemical debridement
Dakin solution
diluted sodium hypochlorite (bleach)
hydrogen peroxide
acetic acid
hypertonic saline
What are two other common chemical agents used for wound care besides Dakin solution?
Hydrogen peroxide, acetic acid
What type of dressings provide effective chemical débridement in early healing stages?
Hypertonic saline dressings
What is the most commonly used enzyme in enzymatic débridement of diabetic foot ulcers?
Collagenase
Which stage must occur before enzymatic débridement for a rigid eschar to be effective?
Sharp débridement
What organism is used in biological débridement?
Lucilia sericata (greenbottle fly larvae)
What is a key advantage of autolytic débridement over other methods?
Least traumatic
Larval secretion has some proprieties name them
debridement
antibacterial effects
promotion of angiogenesis
What must be present for autolytic débridement to be effective?
Moist wound
In wound healing, which stage involves the migration of neutrophils and macrophages?
Débridement stage
What type of fibroblasts are responsible for wound contraction?
Myofibroblasts
The options for wound closure can be categorized in 3. Name them
primary closure
delayed primary closure
second-intention healing
What are the four stages of wound healing
- inflammatory/cellular reaction stage
- debridement stage (sometimes considered part of inflammatory stage)
- tissue formation/proliferation stage
- maturation/remodeling stage
The duration and intensity of the inflammatory stage is determined by
extent of injury
Example: surgical wound has much shorter inflammatory stage than a severe degloving wound
The inflammatory stage can be prolonged by the presence of 3 things, name them
- debris
- foreign material
- infection
The debridement stage begins in the early in the inflammatory stage and is marked by the migration of
neutrophils and macrophages into the wound site
WBC phagocytize bacteria but also enzymatically remove __________ (2w)
necrotic tissue
The debridement stage has lenght that is dependent on the
size of the wound
amount of necrotic debris present
Proliferation or tissue formation stage involves fibroblast migration and proliferation as well as
epitheliazation of the wound
Wound contaction is most effective in areas with
excess of skin
Wound contraction stops when cells of the same type are brought together or when
skin tension equals the ability
Maturation stage of wound healing occurs when an equilibrium between ___________(1w) and production and _______(1w) destruction occurs
collagen
During maturation the number of ______________ (1w) decrease
fibroblasts
The wound contamination is classified based on the degree of contamination. Name them
Clean wound
Celan-contaminated wound
Contaminated wounds
Which wound classification involves gross contamination and necrotic debris?
Contaminated wounds
In the RYB color code, what does black indicate?
Necrotic tissue
Please enunciated the meaing of RYB
The “R” refers to red, and indicates granulation tissue. The “Y” refers to yellow, and indicates purulent debris. The “B” refers to black, and indicates necrotic tissue.
Some wound-care specialists add a “P,” which refers to pink and indicates epithelialization, and others will add a “G,” which refers to green and indicates gangrenous tissue.
What bacterial count generally indicates active infection?
Greater than 10^5 per gram of tissue
Enunciate the difference btw baterial contamination and bacterial colonization
Bacterial contamination describes the presence of bacteria in a wound without active multiplication or trauma to the host. Bacterial colonization indicates that the bacteria have attached to the tissue and are multiplying but not necessarily causing trauma to the host.
Enunciate the meaning of bacterial infection
Bacterial infection occurs when bacteria invade healthy tissue and actively multiply, overwhelming the host’s immune response.
Frequent bandage changes have been implicated with an increase in bacterial numbers, as the wound is left exposed to the environment during the dressing change
What are the 2 types of bacteriologic assesment?
- Qualitative assessment
- Quantitative bacteriology
What are the signs of infection?
Signs such as discolored granulation tissue, edema in and around the wound, purulent exudate, odor, and lameness can indicate an infection.
However, a wound can be infected without these overt signs. If infection is suspected, the wound should be culture
Is quantative common to be performed in veterinary medicine?
No it is qualitative with sensitivity testing. In vet medicine, is not performed but it should be considered when a wound is not progressing as anticipated or when a skin graft fails. Bacterial counts greater than 105 per gram of tissue generally indicate an active infection.
Which three strategies prevent bacterial infection of wounds?
Effective wound cleansing and debridement
Apropriate dressing
Topic antimicrobials
Antiseptic agents, are effective against gram +, gram - or both?
Both, however they do not penetrate necrotic debris and are unlikely to reduce baterial populations in wound bed
Which topical antibiotic is one of the most effective in wound healing?
Triple-antibiotic ointment
Which antiseptic agent should be reconsidered for open wounds due to potential ill effects?
Chlorhexidine
Why should nitrofurazone not be recommended for wound care?
Toxic to wounds
MRSA means
methicillin-resistant Staphylococcus aureus
What is a major difference between antiseptics and topical antimicrobial agents?
topical antimicrobial agents provide efficacy against bacteria within the wound bed and, depending on the vehicle, they have minimal negative side effects on wound healing
Antiseptics cannot target specific bacteria, but topical antibiotics can.
When are the topical antimicrobials ideal?
In chornic infections the blood supply to the surface is diminished and topical antimicrobials are required
What is one of the most effective topical antibiotic in wound healing
Triple-antibiotic ointment
The type of closure technique to use depends on
what caused the wound,
the time from injury,
the degree of contamination,
the extent of the injury,
the potential dead space,
the location of the wound on the patient, and the veterinarian’s surgical skills
Golden period for primary closure
6 hours post trauma
Candidates to primary closure are wounds with certain caratheristics (3)
- only in wounds with minimal tissue loss,
- minimal bacterial contamination,
- minimal tension on the wound edges after closure.
What are the wound closure techniques after sucessful debriment and cleaning?
- Suture closure
- Healing by second intention
- skin grafting
Primary closure is ideal in wounds with certain caracteristics
- minimal tissue loss
- minimal bacterial contamination
- minimal tension on the wound edges after closure
Which surgical tx is the most satisfactory for 1ary closure with apposition of skin edges and at same time tension relief?
Near-far-far-near suture
What are the mattress patterns?
vertical mattress and horizontal mattress
Mattress sutures have ____ (1w) to reduce pull-through at the skin suture interface
stents
4 tx for diminish the dead space in a wound
- suture
- meshing the skin
- passive or active drains
- pressure bandages
Excess suture (too many sutures, too large diamete, too many knots) can potentiate infection - TRUE or FALSE
TRUE
What choice of suture should you give preference to avoid infection
smallest diameter, monofilament, absorbable
Drains can allow evacuation of dead space but also the disadvantage of
codnuit for bacteria to enter the wound
Delayed primary closure is reserved for wounds that have
mild to moderate bacterial contamination
minimal tissue loss
minimal tension on the wound edges
Figure 27-9. A chronic wound that has been débrided and partially closed with near-far-far-near sutures. Delay primary closure
Second intention healing occurs when ?
when wounds have gross contamination and moderate-to-severe tissue loss that would make closure impossible.
What is the concept behind moist wound healing
wound exudate provides necessary cells and a substrate rich in enzymes, growth factors cemotactic factors and provides environment for healing
Enzymes come from what?
Come from breakdown of white blood cells and metalloproteinases
Occlusive dressings keep the wound fluid in contact with the wound _______ (1w) to encourage autolytic debridement
Occlusive dressings keep the wound fluid in contact with the wound bed to encourage autolytic débridement.
Local growth factors and cytokines provide a stimulus for the
fibroblasts, epithelial cells, and angiogenesis
moist environment allows better migration of neutrophils and macrophages than a ________ (1w)wound environment.
moist environment allows better migration of neutrophils and macrophages than a dry wound environment.
Hypertonic saline dressings have been designed for use on
necrotic or heavily exuding wounds
hypertonic saline dressing work by ___________(1w) action to remove necrotic tissue and bacteria
They work by osmotic action to remove necrotic tissue and bacteria
Hypertonic dressings need to be changed every to h
24 to 48 h
Ideal percentage of hypertonic saline and preparation
20% = 200 g of salt in 1 L of hot water
Honey has been used for centuries due to its _______________________bactericidial/bacteriostatic effec
Honey has been used in wound healing for centuries because of its ascribed bactericidal effects
what does it mean PHMB?
active agent is polyhexamethylene biguanide (PHMB)
PHMB belongs to a class of
PHMB belongs to a class of cationic surface-active agents that have been used as preservatives in aqueous solutions and as disinfectants and antiseptics
PHMB when impregnated into fabric has been shown to
have shown the capability to suppress microbial growth and penetration. Microbial death occurs by destabilization and disruption of the cytoplasmic membrane, resulting in leakage of macromolecular components
Silver should be used in clean wounds, infected wounds
infected wound and change bandage every 3 days (open) to 7 days (closed wound)
Wound gel dressings ar ecomposed of water, glycerin, polymers and are ideal for
dry wounds
change 4-7 d
Calcium-alginate dressings are used 1arily for the ________(1w) phase of wound repair
granulating phase
How does it work the calcium from calcium alginate dressings to work?
The calcium in the dressing interacts with sodium in the wound, providing a wound exudate that stimulates myofibroblasts and epithelial cells, and speeds wound homeostasis. The calcium also modulates epithelial cell proliferation and migration.
Calcium alginate dressings have been shown to improve superficial bone healing and avoid bone sequestrum?
yes, Calcium alginate dressings have been shown to improve superficial bone healing and are very effective in reestablishing the periosteum over bones in horses, reducing the possibility of sequestrum formation
Topical dressings such as collagens and maltodextrins are designed for the used in the
A) granulation stage
B) epithialization stage
C) inflamation stage
D) debridement stage
A) granulation stage
What is the purpose of biological dressings?
Cell migration framework
Name two porcine-derived biological dressings.
Small intestinal submucosa (SIS), bladder basement membrane
Which biological dressing reduces granulation tissue and enhances epithelialization?
Porcine SIS
What dressing reduces wound retraction and granulation tissue formation?
Equine amnion