ch 3 pt 2 - dna + cell cycle Flashcards
Protein synthesis
process of creating new proteins from DNA
protein synthesis steps
- It involves the production of mRNA from a gene on DNA, which then attaches to a ribosome for protein synthesis
-The newly synthesized protein is then modified within the RER and transported to the Golgi apparatus for further processing and packaging
dna replication
the process of duplicating the genetic material in the nucleus of a cell accurately
dna replication 5 steps
- Unwinding DNA strands
-Binding of DNA polymerase to exposed nitrogenous bases
-Adding nucleotides to make a complementary strand aka the leading strand
-Assembling a complimentary copy
-Splicing together the 2 dna segments into a strand called the lagging strand
dna transcription
DNA to RNA
synthesis of RNA from a DNA template
what enzyme is used in transcription
RNA polymerase is used to to transcribe DNA
what are the steps of transcription
initiation: RNA polymerase binding
elongation: RNA polymerase nucleotide linking
termination: detachment of mRNA
initiation - rna polymerase binding
RNA polymerase binds to a region of a gene called the promoter
- This signals the DNA to unwind
Elongation - RNA polymerase nucleotide linking
RNA polymerase reads DNA code and builds complementary mRNA by binding nucleotides (contains U instead of T)
Termination - detachment of mRNA
the enzyme and the mRNA strand detach from DNA at the “stop” signal
translation
RNA to protein
synthesis of a polypeptide (chain of amino acids) based on the instructions in mRNA
initiation in translation
mRNA binds to ribosome and mRNA codon translates to one amino acid
elongation in translation
- amino acids are delivered by transfer RNA (tRNA)
- anticodons on tRNA bind to a complementary codons on mRNA
termination in translation
polypeptide chain releases when a stop codon enters the ribosome
where does translation occur
at ribosomes in the cytoplasm