ch 3 pt 2 - dna + cell cycle Flashcards
Protein synthesis
process of creating new proteins from DNA
protein synthesis steps
- It involves the production of mRNA from a gene on DNA, which then attaches to a ribosome for protein synthesis
-The newly synthesized protein is then modified within the RER and transported to the Golgi apparatus for further processing and packaging
dna replication
the process of duplicating the genetic material in the nucleus of a cell accurately
dna replication 5 steps
- Unwinding DNA strands
-Binding of DNA polymerase to exposed nitrogenous bases
-Adding nucleotides to make a complementary strand aka the leading strand
-Assembling a complimentary copy
-Splicing together the 2 dna segments into a strand called the lagging strand
dna transcription
DNA to RNA
synthesis of RNA from a DNA template
what enzyme is used in transcription
RNA polymerase is used to to transcribe DNA
what are the steps of transcription
initiation: RNA polymerase binding
elongation: RNA polymerase nucleotide linking
termination: detachment of mRNA
initiation - rna polymerase binding
RNA polymerase binds to a region of a gene called the promoter
- This signals the DNA to unwind
Elongation - RNA polymerase nucleotide linking
RNA polymerase reads DNA code and builds complementary mRNA by binding nucleotides (contains U instead of T)
Termination - detachment of mRNA
the enzyme and the mRNA strand detach from DNA at the “stop” signal
translation
RNA to protein
synthesis of a polypeptide (chain of amino acids) based on the instructions in mRNA
initiation in translation
mRNA binds to ribosome and mRNA codon translates to one amino acid
elongation in translation
- amino acids are delivered by transfer RNA (tRNA)
- anticodons on tRNA bind to a complementary codons on mRNA
termination in translation
polypeptide chain releases when a stop codon enters the ribosome
where does translation occur
at ribosomes in the cytoplasm
introns
noncoding sequences
mRNA splicing
pre-MRNA transcript is transformed into a mature MRNA. It removes all the introns and splicing back together exons
exons
coding segments
membrane potential
results from unequal distribution of positive and negative charges across the plasma membrane
sodium potassium pump
a protein in the cell membrane that moves sodium and potassium ions in and out of cells, helping to maintain the cell’s osmotic equilibrium and membrane potential
potential difference
occurs when positive and negative charges are separated,
genetic code
Sequence of bases that are a chemical language of DNA instructions of how to build proteins
what are the stages of cell cycle
G1, S, G2, and M
G1 phase
stage where the cell is preparing to divide
S phase
dna replication
G2 phase
organizes and condenses the genetic material
protein synthesis
m phase
mitosis
- the cell actually partitions the two copies of the genetic material into the two daughter cells