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Microbiology > Scientists And Stains > Flashcards

Scientists And Stains Flashcards

1
Q

Who developed Liquid Media

A

Louis Pasteur

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2
Q

Who is the Father of Microbiology

A

Louis Pasteur

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3
Q

Fermentation Principle and Autoclave is discovered by

A

Louis Pasteur

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4
Q

Vaccines developed by Louis Pasteur

A

C - Cholera
A - Anthrax
R - Rabies

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5
Q

Theories given by Louis Pasteur

A

Theory of Abiogenesis - disapproved
Germ Cell Theory

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6
Q

Who is the Father of Modern Microbiology

A

Robert Koch

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7
Q

Who discovered Solid culture media

A

Robert Koch

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8
Q

Who discovered Cholera Organism

A

Robert Koch

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9
Q

Aniline dye and Hanging drop motility test is discovered by

A

Robert Koch

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10
Q

Koch Postulates

A

1)Constant association of causative organism with disease
2)Isolation in culture media possible
3)Culture growth inoculated in animal should produce same lesion
4)Reisolation from experimental animal is possible
5)Antigen of organism should be able to produce Antibody in serum

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11
Q

Exceptions of Koch Postulates

A

L - Mycobacterium Leprae
P - Treponema Pallidum
G - Gonococci

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12
Q

Who is the Father of Chemotherapy

A

Paul Ehrlich

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13
Q

Acid fast stain is first discovered by

A

Paul Ehrlich

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14
Q

Paul Ehrlich got Nobel Prize for

A

Toxin - Antitoxin standardisation

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15
Q

Who is the father of Light Microscopy

A

Anton Von Leeuwenhoek

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16
Q

First thing visualized on Light Microscopy by Anton Von Leeuwenhoek is known as

A

Animal Cules

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17
Q

Who is the father of Antiseptic surgery

A

Joseph Lister - First used Carbolic acid

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18
Q

Who discovered Compound Microscope

A

Jansen Duo

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19
Q

Father of Electron Microscopy

A

Ernst Ruska

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20
Q

First vaccine is produced by

A

Edward Jenner - Smallpox vaccine (prepared by using cowpox)

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21
Q

Gram Staining is discovered by

A

HC Gram

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22
Q

PCR is discovered by

A

Karry B Mullis

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23
Q

L-Forms or cell wall deficient is discovered by

A

Klienberger

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24
Q

Types of Fixation while staining

A

Heat Fixation
Chemical Fixation - Methanol

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25
Examples of Simple stain
1 color Methylene blue (Blue) Basic Fuchsin (Red)
26
Which structures got highlighted in Negative stains
Background stains and Bacteria and Capsule got highlighted
27
Examples of negative Stain
India ink Nigrosin
28
For which organism Negative stains are commonly used
Cryptococcal Meningitis
29
Impregnation stain Principle
Deposit stain on surface of object to make it look thicker
30
Impregnation stains are used for
Thin structures like Flagella Spirochetes - Silver stains
31
Silver stain used for Fluid specimens
Fontana stain
32
Silver stain used for Tissue samples
Levaditi's Stain
33
Types of Differential Stains
Gram Stain Acid Fast stain Albert Stain
34
Gram Staining Sequence
COME IN AND STAIN COME - Crystal Violet (Both Gram +ve and -ve are Purple IN - Iodine (Mordant) AND - Alcohol/Acetone - Decolorizer (G +ve : Purple, Gram -ve : Colorless) STAIN - Safranin Red ( Gram +ve - Purple, Gram -ve : Red/Pink)
35
What is used as Mordant in Gram staining
Iodine
36
What is used as a decoloriser in Gram staining
Alcohol/Acetone
37
Most crucial step in Gram staining
Step of Decolorisation
38
Examples of Poorly Gram Staining Organisms
M - Mycoplasma R - Ricketssia C - Chlamydia S - Spirochetes
39
Steps of Acid fast/ZN Staining
CLASS HAS ASKED MNEMONIC CLASS - Carbol Fuchsin (Red) HAS - Heat (Mordant) ASKED - Acid or Mixture of Alcohol (H2SO4) - decoloriser MNEMONIC - Methylene Blue or Malachite green
40
What will be used as Mordant in ZN Staining
Heat
41
What is used a a decoloriser in Acid Fast/ZN Staining
H2SO4
42
Color of organism and Background in ZN Staining
Organism - Red Background - Blue
43
Amount of H2SO4 to stain Myc. tuberculosis
20% H2SO4
44
Amount of H2SO4 used to stain M. Leprae or Coccidian parasites
5% H2SO4 - Fite Forocco Stain
45
Amount of H2SO4 used to stain Spores
0.25% to 0.5% H2SO4
46
Examples of ZN stain Positive Organisms
My - Mycobacteria (Typical, Atypical, Leprae) Nose - Nocardia, Legionella IS - Isospora Cold - Cyclospora, Cryptosporidium Hot - Hooklet of Hyatid and Eggs of T. Saginata
47
Modification of ZN stain is termed as
Kinyoun Stain/Gabbet Stain
48
Difference between ZN stain and Kinyoun or Cold ZN Stain
Heating process is removed - increased Phenol concentration in Carbol Fuschin
49
Kinyoun Stain or Cold ZN Staining is used for diagnosis of
Coccidian Parasites - Isospora, Cyclospora, Cryptosporidium
50
Albert stain is used to identify
Volutin Granules
51
Volutin Granules can be seen in
C. Diphtheria Yersinia Pestis M. Tb Gardeneralla Spirillum
52
Types of solution used in Albert Stain
Albert Solution 1 - Toluidine Blue, Malachite green, Glacial acetic acid Albert Solution 2 - Iodine
53
Stains used to identify Volutin Granules
P - Pander's Stain L - Loeffler Methylene Blue ( Best) A - Albert STAIN N - Neisser Stain
54
Stain used to identify Flagella
Leifson And Ryu stain
55
Dye used for Leifson And Ryu stain
Basic Fuschin for flagella (Red) Methylene Blue for cell (Blue background)
56
What is used as Mordant in Leifson and Ryu stain
Tannic acid
57
Stain used to identify Spores
Schaeffer and Fulton stain or Modified Ashby stain
58
Dye used in Schaeffer and Fulton stain/Modified Ashby stain
MS SPORE Malachite green Safranin red
59
Color of Bacteria and spores on Modified Ashby stain or Schaeffer and Fulton stain
Bacteria - Red (Safranin) Spores - Green (Malachite green)
60
Stain commonly used to identify Fungi
Methanamine Silver

Microbiology (37 decks)

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