Microscopes Flashcards
Resolution of Eye , Light and Electron Microscope
Eye - 0.2mm
L/M - 0.2 micron
E/M - 0.2-0.5 nm
What is Source in Light Microscope
Transmitted Light
Location of Condenser on Light Microscope
Below the Stage
Big knob and Small knob on Light Microscope are used for
Big Knob - Coarse adjustment
Small knob - Fine Adjustment
Magnification of eyelens
10x Magnification
Types of Objective Lens with Magnification
Scanner - 4x
Low Power - 10x
High Power - 40x
Oil immersion - 100x
Total magnification means
Magnification+ Eyepiece lens magnification
Maximum magnification of Light Microscope
1000x
Source in Dark Field Microscope
Reflected Light
Dark Field Microscope is used to visualize
Thin structures
Spirochetes
Flagella
Interference Contrast Microscope is used for
Identify Cell organelles
Measurement of Chemical constituents such lipid, protein etc
Source for Fluorescence Microscope
UV light
Dyes used to Visualize TB on Fluorescence Microscope
Auramine, Rhodamine
Dye used to visualize Malaria on Fluorescence Microscope
Acridine Orange
Dye used to identify Fungus on Fluorescence Microscope
Calcoflour white
Dye used for Flow Cytometry
FITC (Fluorisothiocynate)
Fluorescence Microscope principle
Converts shorter wavelength into Longer wavelength
Dichroic mirror is used in which Microscope
Fluorescence Microscope
Autofluorescence means
Fluorescent even without adding Fluorescence dye
Autofluorescence property is shown by
Cyclospora, Isospora
Formalin
NADPH - used in Fluorescent spot test (G6PD Deficiency)
Fluorescent source used in Woodlamp examination
UV Light
Different structures in Phase Contrast Microscope in compare to Light Microscope
Annular diaphragm in front of Condenser
Annular phase plate
Source in Electron Microscope
Beam of electrons
Medium used in Electron Microscope
Vacuum
Types of Electron Microscope
Scanning EM
Transmission EM
Solution used for fixation on Light Microscope
10% neutral Buffered Formalin(NBF)
Solution used for fixation in Electron Microscope
2-2.5% Glutaraldehyde
For electron Microscopy Slide is made up of
Metallic (Cu)
Principle of Scanning electron microscope
Scattered electrons
Principle of Transmitted Electron Microscope
Transmitted Electrons
Scanning electron microscope
Principle - Scattered Electrons
Dimensional - 3D
More sample viewed in lesser time
Shows surface structures
Transmission Electron Microscope
Principle - Transmitted Electrons
Dimensional - 2D
Less sample viewed in same time
Shows internal structure
