Microscopes

Question 1

Resolution of Eye , Light and Electron Microscope

Answer 1

Eye - 0.2mm
L/M - 0.2 micron
E/M - 0.2-0.5 nm

Question 2

What is Source in Light Microscope

Answer 2

Transmitted Light

Question 3

Location of Condenser on Light Microscope

Answer 3

Below the Stage

Question 4

Big knob and Small knob on Light Microscope are used for

Answer 4

Big Knob - Coarse adjustment
Small knob - Fine Adjustment

Question 5

Magnification of eyelens

Answer 5

10x Magnification

Question 6

Types of Objective Lens with Magnification

Answer 6

Scanner - 4x
Low Power - 10x
High Power - 40x
Oil immersion - 100x

Question 7

Total magnification means

Answer 7

Magnification+ Eyepiece lens magnification

Question 8

Maximum magnification of Light Microscope

Answer 8

1000x

Question 9

Source in Dark Field Microscope

Answer 9

Reflected Light

Question 10

Dark Field Microscope is used to visualize

Answer 10

Thin structures
Spirochetes
Flagella

Question 11

Interference Contrast Microscope is used for

Answer 11

Identify Cell organelles
Measurement of Chemical constituents such lipid, protein etc

Question 12

Source for Fluorescence Microscope

Answer 12

UV light

Question 13

Dyes used to Visualize TB on Fluorescence Microscope

Answer 13

Auramine, Rhodamine

Question 14

Dye used to visualize Malaria on Fluorescence Microscope

Answer 14

Acridine Orange

Question 15

Dye used to identify Fungus on Fluorescence Microscope

Answer 15

Calcoflour white

Question 16

Dye used for Flow Cytometry

Answer 16

FITC (Fluorisothiocynate)

Question 17

Fluorescence Microscope principle

Answer 17

Converts shorter wavelength into Longer wavelength

Question 18

Dichroic mirror is used in which Microscope

Answer 18

Fluorescence Microscope

Question 19

Autofluorescence means

Answer 19

Fluorescent even without adding Fluorescence dye

Question 20

Autofluorescence property is shown by

Answer 20

Cyclospora, Isospora
Formalin
NADPH - used in Fluorescent spot test (G6PD Deficiency)

Question 21

Fluorescent source used in Woodlamp examination

Answer 21

UV Light

Question 22

Different structures in Phase Contrast Microscope in compare to Light Microscope

Answer 22

Annular diaphragm in front of Condenser
Annular phase plate

Question 23

Source in Electron Microscope

Answer 23

Beam of electrons

Question 24

Medium used in Electron Microscope

Answer 24

Vacuum

Question 25

Types of Electron Microscope

Answer 25

Scanning EM
Transmission EM

Question 26

Solution used for fixation on Light Microscope

Answer 26

10% neutral Buffered Formalin(NBF)

Question 27

Solution used for fixation in Electron Microscope

Answer 27

2-2.5% Glutaraldehyde

Question 28

For electron Microscopy Slide is made up of

Answer 28

Metallic (Cu)

Question 29

Principle of Scanning electron microscope

Answer 29

Scattered electrons

Question 30

Principle of Transmitted Electron Microscope

Answer 30

Transmitted Electrons

Question 31

Scanning electron microscope

Answer 31

Principle - Scattered Electrons
Dimensional - 3D
More sample viewed in lesser time
Shows surface structures

Question 32

Transmission Electron Microscope

Answer 32

Principle - Transmitted Electrons
Dimensional - 2D
Less sample viewed in same time
Shows internal structure