DNA Viruses Part 2 Flashcards
Smallest virus
Parvoviridae - Parvovirus (20nm)
Parvoviridae Features
Smallest virus
ssDNA
Icosahedral Symmetry
Spread of Parvoviridae
Respiratory route
Blood Transfusion
Transplacental transmission
Parvoviridae attacks which antigen
Attack P antigen of RBC precursor
Genotypes and Serotypes of Parvoviridae
3 genotypes - Parvoviridae 1(M/C), 2 and 3
Serotype 1
Conditions associated with Parvovirus B19
Erythema infectiosum (5th disease,slapped chick appearance)
Gloves and socks Syndrome
Arthritis, Arthralgia
Aplastic crisis (in Hemolytic anemia)
Pure red cell aplasia (PRCA)
Hydrops fetalis
Erythema infectiosum Clinical features
AKA 5th disease/Slapped cheek appearance
Macular erythematous rash on cheek
Lacy rash on rest of body
Self limiting condition
Gloves and socks Syndrome also known as
PPGSS - Papular Perpeuric Gloves and Socks Syndrome
Pure red cell aplasia (PRCA) is associated with which conditions
Parvo B19 virus
Thymoma
Diagnosis of Parvoviridae
ELISA For Antibody
PCR for DNA
First disease AKA
Measles
Second disease AKA
Scarlet fever
Third disease AKA
German measles
Fourth disease AKA
DUKE (Obsolete)
Fifth disease AKA
Slapped cheek/Erythema infectiosum
Sixth disease AKA
Roseola infantum/HHV 6
Papovaviridae Family includes
Papilloma virus
Polyoma Virus
Polyoma Virus includes
Merkel cell virus (MCV)
SV40 (Simian Vacuolatin
JC Virus
BK Virus
Merkel cell virus known to cause
Merkel cell carcinoma - Neuroendocrine tumor
CK 7 -ve, CK 20 +ve
SV40 (Simian Vacuolating) is associated with
Non pathogenic to human
Associated with Mesothelioma
JC Virus attacks which cells and causes
Attacks oligodendrocytes
Causes demyelinating syndrome - Progressive Multifocal Leukoencephalopathy (PMLE)
Myelin stain used in PMLE and finding
Luxol fast blue - Negative in PMLE
BK Virus causes
Interstitial Nephritis
Hemorrhagic Cystitis
Usually seen in Post transplant pt.
Urine M/E finding in BK virus
Large cell - Decoy cells (High N/C ratio mimics cancer)
Human Papilloma virus involves which organs
Cutaneous involvement - HPV 1,2,3 (low risk) ; HPV 5,8 (High risk)
Mucosal involvement - HPV 6,11(low risk) ; HPV 16,18,31,33,35 (High risk)
HPV 1,2,3 causes
Low risk
Plantar wart
Filiform wart
HPV 5,8 known to cause
Epidermodysplasia Verruciforms/Tree man Syndrome - a/w With chr. 17
HPV 6,11 Causes
Laryngeal Papillomatosis
Condylomata Acuminatum (Genital warts)
L1 capsid protein of HPV is used for
Used for preparing vaccine
E2 protein of HPV is used as
Controller
E4 and E5 Protein of HPV known to show which structure
Koilocytosis - Koilocyte +ve
E6 and E7 Protein of HPV is responsible for
Carcinogenesis - Squamous epithelium - Cervical cancer, Penile cancer, Anal canal cancer, Oral cancer, Laryngeal Cancer, esophageal cancer)
E6 protein inactivates which gene
P53 (Policeman)
E7 protein of HPV inactivates which gene
RB gene (Governer)
Diagnosis of HPV Infection
Screening - Pap smear
Confirmatory - Biopsy (Koilocyte +)
Molecular tests - PCR, Hybrid capture assay
Vaccines for HPV
Cervarix
Gardasil
Gardasil 9
Cervarix vaccine adjuvant and provides protection against
Bivalent
Adjuvant - ASO4 (Act on TLR’s - enhances immunity)
Protection - HPV 16,18
Gardasil vaccine provides protection against
Quadrivalent
Protection against HPV 6,11,16,18
Poxviridae family includes
Vaccinia (Cowpox)
Variola (Smallpox)
Molluscum Contagiosum
Monkey pox
Size, shape, symmetry, and replication site in Poxviridae family
Size - 300nm (Largest)
Shape - Box/brick With Dumbell DNA core
Symmetry - Complex
Replication site - Cytoplasm
Variola/small pox incubation period
12 days
Crop and fever in case of Smallpox
No crop
No fever with crop - fever subsides with rash
Distribution of rash in Smallpox
Centrifugal (from extremities) to centripetal (Trunk)
Vaccine used against Variola/Smallpox
1st vaccine - Edward Jenner(Using cowpox)
Live vaccinia vaccine - freeze dried preparation
Cytopathic effect shown by Variola and Vaccinia
Vaccinia - Guarnieri body
Variola - Paschen body
Vaccinia on Chorioallantoic Membrane shows
Large, hemorrhagic necrotic lesions
Variola on Chorioallantoic Membrane shows
Small, non hemorrhagic lesions
Molluscum Contagiosum is most commonly caused by
Molluscum contagious virus 1 (M/C) and 2
Transmission of Molluscum contagious virus
In child and adults - by contact
Characteristic Findings of Molluscum Contagiosum lesions
Tam color umbilicated lesions - on pressing, curdy discharge
Seen all over body except Palms, soles
Cytopathic effect caused by Molluscum Contagiosum virus
Cup shaped
Henderson Patterson bodies (HP Body)
Stained used to see Henderson Patterson bodies
Phloxine Tartarate stain - also used to stain Paneth cells (GIT)
E/M finding in case of Molluscum contagious virus
Brick/box shape with Dumbell core
Reservoir of Monkeypox
Rodents
Mode of transmission of Monkey pox
Zoonotic - bites, scratches, Consumption of bush meat
Human to human transmission possible
Incubation period and clinical features of Monkey pox
IP - 2 weeks
C/F - Fever,Rash, Lymphadenopathy
Shape of Adenovirus
Space vehicle shape
Fibers rising from surface of Adenovirus helps in
Attachment
Clinical features caused by Adenovirus
Hemorrhagic Cystitis
Infant diarrhea
Involvement of Eyes
Respiratory diseases
Which type of Adenovirus involved in case of Hemorrhagic Cystitis
11,21
Type of Adenovirus responsible for Infant diarrhea
40,41
Clinical features related to eyes caused by Adenovirus
Epidemic Keratoconjunctivitis/Shipyard eye
Pharyngoconjunctival fever/swimming pool Conjunctivitis
Epidemic Keratoconjunctivitis/Shipyard eye caused by which adenovirus
AKA Epidemic follicular conjunctivitis - Corneal opacities
Type 8,19,37
Pharyngoconjunctival fever/ swimming pool Conjunctivitis clinical features and types involved
H/O swimming
Pharyngitis
Follicular conjunctivitis (U/L or B/L)
In children’s
Respiratory diseases caused by Adenovirus and types involved
URTI - 1,2,3,5
Pneumonia - 3,7,21
Transplant recipients - 34,35
