COVID 19 And miscallenous Viruses

Question 1

Family and genus of Coronavirus

Answer 1

Family - Coronaviridae
Genus - Beta coronavirus

Question 2

Features of Coronavirus

Answer 2

Non segmented
Positive sense RNA
ssRNA

Question 3

Structure present in Coronavirus

Answer 3

Spike (S1 and S2) - helps in attachment
Nucleocapsid (N)
Membrane (M)
Envelope (E)

Question 4

Coronavirus attaches to which receptors and location

Answer 4

1) Spike proteins attaches on ACE2 Receptors
Maximally present in Lungs (type 2 pneumocytes), GIT, Heart, Kidney

Question 5

Activation of spike protein in coronavirus happens through

Answer 5

TMPRSS2

Question 6

Pathogenesis of COVID 19

Answer 6

Attachment - Endocytosis - Uncoating - Viral Replication - Viral assembly and Release

Question 7

When Max shedding in COVID-19 occurs

Answer 7

Occurs 5-8 hrs before onset of symptoms

Question 8

Clinical features of COVID-19

Answer 8

Fever
Dyspnea
Dry cough
Shortness of breath
Loss of smell and taste
GI Complaints
Myalgia

Question 9

Radiological findings seen in COVID-19

Answer 9

Multifocal ground glass opacities
Consolidation
Pavement appearance (multiple GGO’s with septal thickening)
Reverse halo sign

Question 10

Collection of COVID-19 sampe

Answer 10

Upper respiratory specimen - Nasopharyngeal and oropharyngeal swab
Lower resp specimen - BAL, ET Aspirate, sputum

Question 11

Gold standard method of diagnosis of COVID-19 and turn around time

Answer 11

Real time RT PCR
Turn around time - 4-6 hrs

Question 12

Semi confirmatory genes for COVID-19

Answer 12

F gene
N gene
S gene

Question 13

Confirmatory genes for COVID-19

Answer 13

RdRP gene
ORF1 alpha gene

Question 14

CBNAAT or True NAAT procedure in case of COVID 19

Answer 14

Portable PCR <30 min
2 steps - E gene screening (semi confirmatory gene) - If +ve - Check RdRP(Confirmatory) - If -ve - RRTPCR

Question 15

Rapid card test for COVID-19 principle is based on

Answer 15

Immunochromtatography

Question 16

FELUDA test

Answer 16

Fn Cas9 Editor linked uniform detection assay
Principle - Crispr Cas9
Paper based Test strip
Time <45 min

Question 17

Reservoir for Arenaviridae family

Answer 17

Rodents

Question 18

Arenaviridae features

Answer 18

Negative sense RNA
Segmented RNA Virus - 2 segments
Bioterrorism A agent
Sand sprinkled /sandy appearance

Question 19

Arenaviridae family includes

Answer 19

Lassavirus
Junin virus
Machupovirus
Lymphocytic choriomeningitis virus

Question 20

Which Arenaviridae family members known to cause Hemorrhagic fever

Answer 20

Lassavirus
Junin virus
Machupovirus

Question 21

Lymphocytic choriomeningitis virus Causes

Answer 21

Meningitis

Question 22

Reoviridae family includes

Answer 22

Rotavirus

Question 23

Rotavirus release which toxin

Answer 23

NSP4 Enterotoxin

Question 24

Structural protein of Rotavirus

Answer 24

VP 1-4,6,7

Question 25

Non structural proteins for Rotavirus

Answer 25

NSP 1-6

Question 26

Serotypes of Rotavirus and most common one

Answer 26

A to I - Serogroup A is most common cause of diarrhea in children of less than 5 years

Question 27

IP and Transmission route of Rotavirus

Answer 27

Feco oral route IP - 1 to 3 days Small infectivity dose

Question 28

Rotavirus shape on E/M

Answer 28

Rotating wheel shape

Question 29

Vaccine of Rotavirus and side effect

Answer 29

Rotateq Rotatrix S/E - Intussception

Question 30

Which is the only dsRNA virus

Answer 30

Reo/Rotavirus Segmented - 11 segments

Question 31

Filoviridae family includes

Answer 31

Ebola virus Marburg virus

Question 32

Ebola virus shape and transmission

Answer 32

Bowl of spaghetti/Filamentous Spread - secretions, contact

Question 33

Marburg virus is known to cause

Answer 33

Hemorrhagic fever

Question 34

Reservoir host of Ebola virus

Answer 34

Bats

Question 35

Species of Ebola virus and virulence

Answer 35

Zaire ebola - Most virulent Ivory coast and - least virulent Reston - not known to cause disease

Question 36

Incubation period of Ebola virus

Answer 36

3-14 days

Question 37

Treatment of Ebola virus

Answer 37

Supportive Inmazeb - Combination of Atoltivimab, Maftivimab, Odesivimab

Question 38

Vaccine for Ebola virus infection

Answer 38

Ebola zaire vaccine

Question 39

Viruses causing gasteroenteritis

Answer 39

Adenovirus 40,41 Rotavirus Norwalk virus (Noroviridae) Calcivirus (Hep E) Astrovirus

Question 40

Hemorrhagic fevers Mnemonic

Answer 40

AB Hoga FFvR Arena virus Bunyavirus - Crimian congo, Rift valley fever, Hantavirus Flavivirus - Dengue, Yellow fever, KFD Filovirus - Ebola, Marburg virus Reovirus - Colorado tick fever