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BACTERIOLOGY > Obligate intracellular Nonculturabl > Flashcards

Obligate intracellular Nonculturabl Flashcards

1
Q

Energy parasites/Obligate Intracellular, like gram-negative in a way that they have LPS as well in their cell wall

A

Chlamydia

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2
Q

Antigenic variations of Chlamydia spp is due to

A

MOMP (Major Outer Membrane Protein)

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3
Q

Metabolically Inert, Infective form, Inactive form of Chlamydia spp

A

Elementary body

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4
Q

Intracellular, Active Replicating form of Chlamydia spp

A

Reticulate body

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5
Q

C. trachomatis serovars for ocular trachoma

A

A, B, C

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6
Q

C. trachomatis serovar for Pelvic inflammatory disease

A

D to K

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7
Q

C. trachomatis - Lymphogranuloma venereum

A

Serovars L1, L2, L3

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8
Q

1 Non-Gonococcal urethritis, #1 STD in U.S., Trachoma and Inclusion Conjunctivitis agent, Fits-Hugh-Curtis syndrome

A

C. trachomatis

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9
Q

Best test for C. trachomatis

A

PCR/NAAT

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10
Q

Best media for C. trachomatis

A

McCoy Cell Line

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11
Q

Growth enhancer for C. trachomatis

A

Cycloheximide

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12
Q

C. trachomatis specimen collection

A

plastic/metal shafts of swab (Endocervix, Vaginal, Urethral)

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13
Q

Halberstadler-Prowazeik bodies - Glycogen-containing bodies

A

C. trachomatis

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14
Q

Stain for C. trachomatis

A

Lugol’s iodine

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15
Q

Test for Chlamydia antibody

A

Microimmunofluorescence

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16
Q

Bacteria associated with Psittacosis, Ornithosis, Parrot fever, Pneumonia (Hacking cough) from inhalation of bacteria in bird’s droppings/feces

A

C. psittaci

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17
Q

Sample of choice for C. psittaci

A

Sputum, NPS, OPS

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18
Q

Media for C. psittaci

A

Mice or chick embryo

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19
Q

Tests for C. psittaci

A

Microimmunofluorescence and PCR

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20
Q

Inclusion bodies seen in C. psittaci

A

Levinthal-Lillicole bodies - Non-Glycogen containing

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21
Q

Stain for C. psittaci

A

Macchiavello and Giemsa

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22
Q

Bacteria associated with TWAR strain, Taiwanese Acute Respiratory Syndrome, associated with asthma and atherosclerosis

A

Chlamydophila pneumoniae

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23
Q

Sample of choice for Chlamydophila pneumoniae

A

Sputum, NPS, OPS, bronchial wash

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24
Q

Media of choice for Chlamydophila pneumoniae

A

HeLa or Hep-2

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25
Q

Test of choice for Chlamydophila pneumoniae

A

Microimmunofluorescence and PCR

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26
Q

Stain of choice for C. pneumoniae

A

Giemsa

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27
Q

Bacteria associated with TWAR strain of pneumonia

A

Chlamydophila pneumoniae

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28
Q

Bacteria associated with currant jelly-like sputum

A

Klebsiella pneumoniae

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29
Q

Bacteria associated with rust-colored sputum, #1 community-acquired pneumonia

A

Streptococcus pneumoniae

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30
Q

Bacteria associated with Primary Atypical Pneumonia (PAP)

A

Mycoplasma pneumoniae

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31
Q

Bacteria associated with Broadstreet pneumonia

A

Legionella pneumophila

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32
Q

Bacteria with cell wall like a Gram-negative cell wall, Obligate intracellular, and non-cultivable in cell-free media

A

Rickettsia

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33
Q

Attachment to host cell of Rickettsia

A

OMP A and OMP B

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34
Q

Infection occurs after a bite of an infected arthropod vector

A

Rickettsia

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35
Q

Test for Rickettsia

A

NAAT (PCR) or Serology (e.g. Weil-Felix)

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36
Q

Spotted fever group of Rickettsia

A

R. africae, R. conorii, R. helionjiangesis, R. honei, R. japonica, R. rickettsi, R. parkeri, R. sibirica, R. slovaca

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37
Q

Typhus group of Rickettsia

A

R. prowazekii, R. typhi

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38
Q

Scrub typhus group

A

Orientia tsutsugamushi

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39
Q

Causative agent of African tick bite fever

A

R. africae

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40
Q

Causative agent of Mediterranean/Israeli/Boutonneuse spotted fever; Indian/Kenya tick typhus

A

R. conorii

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41
Q

Causative agent of Far-Eastern tick-borne rickettsiosis

A

R. helionjiangesis

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42
Q

Causative agent of Flinders Island spotted fever

A

R. honei

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43
Q

Causative agent of Japanese spotted fever

A

R. japonica

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44
Q

Causative agent of Rocky Mountain spotted fever

A

R. rickettsi

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45
Q

Causative agent of Mild illness of spotted fever group

A

R. parkeri

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46
Q

Causative agent of North Asia tick typhus

A

R. sibirica

47
Q

Causative agent of Tick-borne lymphadenopathy

A

R. slovaca

48
Q

Vector of spotted fever group

A

Ticks

49
Q

Causative agent of Epidemic typhus and Brill-Zinsser disease (Recrudescent disease)

A

R. prowazekii

50
Q

Vector of R. prowazekii

A

Lice

51
Q

Causative agent of murine typhus

A

R. typhi

52
Q

Vector of R. typhi

A

Fleas

53
Q

Scrub typhus causative agent

A

Orientia tsutsugamushi

54
Q

Scrub typhus vector

A

Chiggers

55
Q

Causative agent of Human MONOCYTIC ehrlichiosis

A

Ehrlichia chaffeensis

56
Q

Causative agent of Human GRANULOCYTIC anaplasmosis

A

Anaplasma

57
Q

Vectors of E. chaffeensis

A

Ticks (Ambylomma spp., Lone star tick)

58
Q

Vector of Anaplasma

A

Ticks (Ixodes spp.)

59
Q

Causative agent of sennetsu fever

A

Neorickettsia sennetsu

60
Q

Neorickettsia sennetsu vector

A

Ticks

61
Q

Gram negative coccobacillus which causes Q (Query) fever and can be Isolated from animals

A

Coxiella burnetti

62
Q

Survives extracellularly and can only be grown in lung cells

A

Coxiella burnetti

63
Q

Coxiella burnetti highly infectious - Phase

A

Phase I (Large cell variant)

64
Q

Phase II of C. burnetti

A

Acting like a spore, extracellular (in the environment), Zoonotic infection with cattle, sheep, & goats

65
Q

Biosafety level for C. burnetti

A

Level 3

66
Q

Tests for C. burnetti

A

Shell vial culture using Human lung fibroblast, Serology - most common diagnostic tool, IFA - Reference method, PCR - more sensitive than serology

67
Q

General characteristics: Long, slender, helically curved microaerophilic organism

A

Spirochetes

68
Q

Platelet-like structure in the cell wall in which fibrils are attached in spirochetes

A

Insertion disks

69
Q

Flagella-like organelles wrapped around the bacterial cell wall

A

Axial fibrils/Axial filaments/Endoflagella

70
Q

Tight coiled bacteria

A

Treponema

71
Q

Thicker loose coil bacteria

A

Borrelia

72
Q

Thicker, Loose coil, Hooked ends (question mark appearance)

A

Leptospira

73
Q

Comma-shaped/Helical, Tapered ends with 4 flagella on each end

A

Brachyspira (causes fecal, oral, ulcer abscess)

74
Q

Hutchinson’s triad

A

Deafness, Blindness, Notched peg-shaped teeth

75
Q

Congenital syphilis causative agent

A

T. pallidum subs. pallidum

76
Q

Spx for T. pallidum subs. pallidum in congenital syphilis

A

Blood or CSF

77
Q

Test for T. pallidum subs. pallidum

A

X-ray, Immunologic test

78
Q

Signs and symptoms associated with T. pallidum subs. pallidum

A

Hutchinson’s triad, Bone malformations: Bulldog maxilla, Saber shin (tibia)

79
Q

Primary syphilis signs and symptoms

A

Hunterian chancre/Hard Chancre in genitals

80
Q

Spx for primary syphilis

A

Lesion, Swab/Aspirate

81
Q

Test for primary syphilis

A

Dark-Field microscope, Culture

82
Q

Condylomata lata rashes in palms and soles stage of syphilis

A

Secondary stage

83
Q

Spx for secondary syphilis

A

Lesion aspirate, Serum

84
Q

Test for secondary syphilis

A

Serology

85
Q

Latent syphilis signs and symptoms

A

Not evident

86
Q

Spx of choice for latent syphilis

A

Serum

87
Q

Latent syphilis test

A

Serology

88
Q

Tertiary syphilis signs and symptoms

A

Tissue-destruction; Granuloma-like lesions (Gummas). Cardiac and CNS problems, Neurosyphilis

89
Q

Specimen for tertiary syphilis

A

Lesion, Blood, CSF, Serum, Tissues

90
Q

Test for tertiary syphilis

A

Serology, PCR – Neurosyphilis in AIDS patient

91
Q

Relapsing fever causative agent

A

Borrelia spp

92
Q

Stained blood smear for B. burgdorferi

A

Giemsa stained

93
Q

1st week of infection with Leptospirosis spx

A

Blood and CSF

94
Q

2nd week of infection with Leptospirosis spx

A

Urine

95
Q

Culture media for leptospirosis

A

Fletcher, Noguchi, EMJH media

96
Q

Gold standard for leptospirosis

A

Microscopic agglutination (MIT/MAT)

97
Q

Lyme’s disease blood stain used

A

Acridine orange or Giemsa

98
Q

Biopsy stain for Lyme’s disease

A

Warthin-Starry stain

99
Q

Spx for Lyme’s disease

A

Blood, biopsy, CSF, urine

100
Q

Culture for Lyme’s disease

A

Chick embryo, Barbour-Stoenner-Kelly media

101
Q

Confirmatory for Lyme’s disease

A

Western blot

102
Q

Lyme’s disease causative agent

A

B. burgdorferi

103
Q

Treponema pallidum subsp. pallidum

A

Syphilis

104
Q

Treponema pallidum subsp. pertenue

A

Yaws or Fambresia tropica

105
Q

Treponema pallidum subsp. endemicum

A

Bejel or Endemic Non-venereal syphilis

106
Q

Treponema carateum

A

Pinta or Carate

107
Q

Treponema denticola and Treponema socranski

A

Gingivitis, Periodontitis

108
Q

Borrelia recurrentis

A

Louse-borne/Epidemic/European relapsing fever

109
Q

Borrelia hermsii, B. turicatae, B. duttoni, B. parkeri

A

Tick-borne/Endemic/American relapsing fever

110
Q

Borrelia burgdorferi

A

Lyme’s disease

111
Q

Leptospira interrogans

A

Leptospirosis / Infectious Jaundice / Weil’s disease

112
Q

Stages of Lyme’s disease - First stage

A

Bull’s eye rash (erythema chronicum migrans), fever, malaise, joint pain

113
Q

Stages of Lyme’s disease - Second stage

A

Neurologic disorders (nerve palsy), meningitis

114
Q

Stages of Lyme’s disease - Third stage

A

Acrodermatitis chronica atrophicans (recurring chronic arthritis), Demyelination symptoms of Alzheimer’s disease and Multiple sclerosis

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