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BACTERIOLOGY > Biochemical Test for Mycobacterium > Flashcards

Biochemical Test for Mycobacterium Flashcards

1
Q

Reagents/materials for Semi-Quantitative Catalase test (SQC)

A

30% H2O2 and 10% Tween 80

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2
Q

Positive QC organism for SQC (>45 mm column of bubbles)

A

M. flavescens

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3
Q

Negative QC organism for SQC (<45 mm column of bubbles)

A

M. tuberculosis

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4
Q

Mycobacteria positive for SQC

A

M. terrae, Scotochromogens, Rapid growers, Photochromogens except M. marinum

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5
Q

Reagents/materials for 68°C Heat-stable Catalase test

A

30% H2O2, 10% Tween 80, Water bath

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6
Q

Positive QC organism for 68°C Heat-stable Catalase test (Bubble formation)

A

M. flavescens

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7
Q

Negative QC organism for 68°C Heat-stable Catalase test (No change)

A

M. tuberculosis

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8
Q

Mycobacteria positive for 68°C Heat-stable Catalase test

A

M. terrae, Scotochromogens, M. fortuitum, S. smegmatis, M. xenopi, M. ulcerans, Photochromogens except M. marinum

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9
Q

Reagents/materials for Niacin accumulation

A

Lowenstein-Jensen with ≥50 colonies

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10
Q

Positive QC organism for Niacin accumulation (Yellow)

A

M. tuberculosis

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11
Q

Negative QC organism for Niacin accumulation (No color change)

A

M. fortuitum

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12
Q

Mycobacterium lacking niacin enzyme

A

M. tuberculosis”Reagents/Materials for Arylsulfatase test

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13
Q

Positive QC organism for Arylsulfatase test (Pink in 3 or 14 days)

A

M. fortuitum

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14
Q

Negative QC organism for Arylsulfatase test (No color change)

A

M. tuberculosis

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15
Q

Mycobacteria positive for Arylsulfatase test (3 and 14 days)

A

M. fortuitum, M. chelonae, M. xenopi

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16
Q

Mycobacteria positive for Arylsulfatase test (14 days only)

A

M. marinum

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17
Q

Reagents/Materials for Tween 80 hydrolysis

A

Indicator: Phenol red

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18
Q

Positive QC organism for Tween 80 hydrolysis (Pink color in 10 days)

A

M. kansasii

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19
Q

Negative QC organism for Tween 80 hydrolysis (No color change)

A

M. intracellulare

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20
Q

Mycobacteria positive for Tween 80 hydrolysis

A

M. flavescens, M. gordonae, M. terrae, M. smegmatis, M. kansasii, M. marinum

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21
Q

End products of Tween 80 hydrolysis

A

Oleic acid and Sorbitol

22
Q

Reagents/Materials for Tellurite reduction

A

Potassium tellurite

23
Q

Positive QC organism for Tellurite reduction (Black metallic ppt)

A

M. intracellulare

24
Q

Negative QC organism for Tellurite reduction (No color change)

A

M. kansasii

25
Mycobacteria positive for Tellurite reduction
M. avium complex (MAC), M. simiae
26
Reagents/Materials for T2H inhibition
Culture media with T2H
27
Positive QC organism for T2H inhibition (No growth, Sensitive)
M. bovis
28
Negative QC organism for T2H inhibition (Growth, Resistant)
M. tuberculosis
29
Remarks for T2H inhibition
Use along with nitrate and niacin tests
30
Reagents/Materials for Pyrazinamidase test
Ferric ammonium sulfate and Pyrazinoic acid
31
Positive QC organism for Pyrazinamidase test (Red/Pink band in 4 days)
M. intracellulare
32
Negative QC organism for Pyrazinamidase test (Colorless/Gray)
M. bovis
33
Mycobacteria positive for Pyrazinamidase test
M. tuberculosis, M. avium, M. marinum
34
End products of Pyrazinamidase test
Pyrazinoic acid and Ammonia
35
Reagents/materials for Nitrate reduction
Nutrient broth with 1% Potassium nitrate; Indicator: Sulfanilic acid
36
Positive QC organism for Nitrate reduction (Red)
M. tuberculosis
37
Negative QC organism for Nitrate reduction (No color change)
M. marinum
38
Mycobacteria positive for Nitrate reduction
M. tuberculosis, M. kansasii, M. szulgai, M. flavescens, M. terrae, M. fortuitum
39
Reagents/Materials for Iron-uptake test
20% ferric ammonium citrate
40
Positive QC organism for Iron-uptake test (Rusty brown colonies in egg-based media)
M. smegmatis, M. fortuitum
41
Negative QC organism for Iron-uptake test (No color change)
The rest of Mycobacteria
42
Remarks for Iron-uptake test
M. smegmatis and M. fortuitum are rapid growers
43
Reagents/Materials for Urease test
Urea broth
44
Positive QC organism for Urease test (Pink/Red in 3 days)
M. tuberculosis
45
Negative QC organism for Urease test (No color change)
M. gordonae
46
Mycobacteria positive for Urease test
M. tuberculosis, M. kansasii, M. marinum
47
Reagents/Materials for Salt tolerance test
5% NaCl added to egg-based media
48
Positive QC organism for Salt tolerance test (Substantial growth)
M. fortuitum
49
Negative QC organism for Salt tolerance test (No growth)
M. gordonae
50
Mycobacteria positive for Salt tolerance test
M. fortuitum, M. flavescens

BACTERIOLOGY (49 decks)

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