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BACTERIOLOGY > Biochemical Test for Mycobacterium > Flashcards

Biochemical Test for Mycobacterium Flashcards

1
Q

Reagents/materials for Semi-Quantitative Catalase test (SQC)

A

30% H2O2 and 10% Tween 80

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2
Q

Positive QC organism for SQC (>45 mm column of bubbles)

A

M. flavescens

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3
Q

Negative QC organism for SQC (<45 mm column of bubbles)

A

M. tuberculosis

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4
Q

Mycobacteria positive for SQC

A

M. terrae, Scotochromogens, Rapid growers, Photochromogens except M. marinum

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5
Q

Reagents/materials for 68°C Heat-stable Catalase test

A

30% H2O2, 10% Tween 80, Water bath

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6
Q

Positive QC organism for 68°C Heat-stable Catalase test (Bubble formation)

A

M. flavescens

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7
Q

Negative QC organism for 68°C Heat-stable Catalase test (No change)

A

M. tuberculosis

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8
Q

Mycobacteria positive for 68°C Heat-stable Catalase test

A

M. terrae, Scotochromogens, M. fortuitum, S. smegmatis, M. xenopi, M. ulcerans, Photochromogens except M. marinum

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9
Q

Reagents/materials for Niacin accumulation

A

Lowenstein-Jensen with ≥50 colonies

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10
Q

Positive QC organism for Niacin accumulation (Yellow)

A

M. tuberculosis

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11
Q

Negative QC organism for Niacin accumulation (No color change)

A

M. fortuitum

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12
Q

Mycobacterium lacking niacin enzyme

A

M. tuberculosis”Reagents/Materials for Arylsulfatase test

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13
Q

Positive QC organism for Arylsulfatase test (Pink in 3 or 14 days)

A

M. fortuitum

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14
Q

Negative QC organism for Arylsulfatase test (No color change)

A

M. tuberculosis

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15
Q

Mycobacteria positive for Arylsulfatase test (3 and 14 days)

A

M. fortuitum, M. chelonae, M. xenopi

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16
Q

Mycobacteria positive for Arylsulfatase test (14 days only)

A

M. marinum

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17
Q

Reagents/Materials for Tween 80 hydrolysis

A

Indicator: Phenol red

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18
Q

Positive QC organism for Tween 80 hydrolysis (Pink color in 10 days)

A

M. kansasii

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19
Q

Negative QC organism for Tween 80 hydrolysis (No color change)

A

M. intracellulare

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20
Q

Mycobacteria positive for Tween 80 hydrolysis

A

M. flavescens, M. gordonae, M. terrae, M. smegmatis, M. kansasii, M. marinum

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21
Q

End products of Tween 80 hydrolysis

A

Oleic acid and Sorbitol

22
Q

Reagents/Materials for Tellurite reduction

A

Potassium tellurite

23
Q

Positive QC organism for Tellurite reduction (Black metallic ppt)

A

M. intracellulare

24
Q

Negative QC organism for Tellurite reduction (No color change)

A

M. kansasii

25
Q

Mycobacteria positive for Tellurite reduction

A

M. avium complex (MAC), M. simiae

26
Q

Reagents/Materials for T2H inhibition

A

Culture media with T2H

27
Q

Positive QC organism for T2H inhibition (No growth, Sensitive)

28
Q

Negative QC organism for T2H inhibition (Growth, Resistant)

A

M. tuberculosis

29
Q

Remarks for T2H inhibition

A

Use along with nitrate and niacin tests

30
Q

Reagents/Materials for Pyrazinamidase test

A

Ferric ammonium sulfate and Pyrazinoic acid

31
Q

Positive QC organism for Pyrazinamidase test (Red/Pink band in 4 days)

A

M. intracellulare

32
Q

Negative QC organism for Pyrazinamidase test (Colorless/Gray)

33
Q

Mycobacteria positive for Pyrazinamidase test

A

M. tuberculosis, M. avium, M. marinum

34
Q

End products of Pyrazinamidase test

A

Pyrazinoic acid and Ammonia

35
Q

Reagents/materials for Nitrate reduction

A

Nutrient broth with 1% Potassium nitrate; Indicator: Sulfanilic acid

36
Q

Positive QC organism for Nitrate reduction (Red)

A

M. tuberculosis

37
Q

Negative QC organism for Nitrate reduction (No color change)

A

M. marinum

38
Q

Mycobacteria positive for Nitrate reduction

A

M. tuberculosis, M. kansasii, M. szulgai, M. flavescens, M. terrae, M. fortuitum

39
Q

Reagents/Materials for Iron-uptake test

A

20% ferric ammonium citrate

40
Q

Positive QC organism for Iron-uptake test (Rusty brown colonies in egg-based media)

A

M. smegmatis, M. fortuitum

41
Q

Negative QC organism for Iron-uptake test (No color change)

A

The rest of Mycobacteria

42
Q

Remarks for Iron-uptake test

A

M. smegmatis and M. fortuitum are rapid growers

43
Q

Reagents/Materials for Urease test

A

Urea broth

44
Q

Positive QC organism for Urease test (Pink/Red in 3 days)

A

M. tuberculosis

45
Q

Negative QC organism for Urease test (No color change)

A

M. gordonae

46
Q

Mycobacteria positive for Urease test

A

M. tuberculosis, M. kansasii, M. marinum

47
Q

Reagents/Materials for Salt tolerance test

A

5% NaCl added to egg-based media

48
Q

Positive QC organism for Salt tolerance test (Substantial growth)

A

M. fortuitum

49
Q

Negative QC organism for Salt tolerance test (No growth)

A

M. gordonae

50
Q

Mycobacteria positive for Salt tolerance test

A

M. fortuitum, M. flavescens

BACTERIOLOGY (49 decks)

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