Indicators of culture media and reagents

Question 1

Gram Positive Aerobic Cocci (SMS)

Answer 1

Staphylococcus, Micrococcus, Streptococcus

Question 2

Gram Positive Anaerobic Cocci

Answer 2

Peptostreptococcus, Peptococcus

Question 3

Gram Positive Aerobic Bacilli

Answer 3

Mycobacterium, Corynebacterium, Nocardia, Arcanobacterium, Streptomyces, Rhodococcus, Listeria, Erysipelothrix, Bacillus

Question 4

Gram Positive Anaerobic Bacilli

Answer 4

Clostridium, Cutibacterium, Propionobacterium, Lactobacillus, Actinomyces, Bifidobacterium (dog bone appearance)

Question 5

Gram Negative Aerobic Cocci

Answer 5

Moraxella (Branhamella)

Question 6

Gram Negative Anaerobic Cocci

Answer 6

Veillonella

Question 7

Gram Negative Capnophile Cocci

Answer 7

Neisseria

Question 8

Gram Negative Aerobic Bacilli

Answer 8

Enterobacteria, Pseudomonas, Acidovorax, Burkholderia, Sphingomonas, Vibrio, Aeromonas, Alcaligenes, Francisella, Stenotrophomonas, Acinetobacter, Pasteurella, Bordetella, Brucella, Bartonella

Question 9

Gram Negative Anaerobic Bacilli (BFPP)

Answer 9

Bacteroides, Fusobacterium, Porphyromonas, Prevotella

Question 10

Gram Negative Microaerophile Bacilli

Answer 10

Campylobacter, Helicobacter, Treponema, Borrelia, Leptospira

Question 11

Indicator for XLD, TSI, KIA, MSA, CTA, viral cell culture

Answer 11

Phenol red

Question 12

Indicator for CIN, MAC, SSA, Ashdown Agar

Answer 12

Neutral red

Question 13

Indicator for citrate agar, HEA, OF tube, TCBS

Answer 13

Bromothymol blue

Question 14

Indicator for LIA, 6.5% NaCl

Answer 14

Bromcresol purple

Question 15

Indicator for DNAse agar

Answer 15

Methylene green/Toluidine blue

Question 16

Indicator for anaerobiosis

Answer 16

Resazurin/Methylene blue

Question 17

Media for H2S detection

Answer 17

BSA, KIA, Lead acetate paper strip, SIM, HEA, SSA, LIA, TSI

Question 18

H2S positive bacteria

Answer 18

Erysipelothrix (Gram +), Proteus, Salmonella, Edwardsiella, Brucella abortus, Citrobacter freundii, Shewanella putrefaciens

Question 19

H2S indicator of BSA and KIA

Answer 19

Ferrous sulfate

Question 20

H2S indicator of Lead acetate paper strip and SIM

Answer 20

Lead acetate

Question 21

H2S indicator of HEA, SSA, LIA, TSI

Answer 21

Ferric ammonium citrate

Question 22

Taxo A chemical name

Answer 22

0.04 U Bacitracin

Question 23

Use of Taxo A

Answer 23

Identifying S. pyogenes

Question 24

Taxo P chemical name

Answer 24

Ethyhydrocupreine hydrochloride

Question 25

Use of Taxo P

Answer 25

Identifying S. pneumoniae

Question 26

NALC name

Answer 26

N-acetyl-L-Cysteine A.K.A. Dithiothreitol

Question 27

Use of NALC

Answer 27

Digestion reagent for TB sputum samples

Question 28

ZTP

Answer 28

Zephiran-Trisodium phosphate

Question 29

Use of ZTP

Answer 29

Digestion-Decontamination reagent for TB sputum samples

Question 30

Tween 80

Answer 30

Polyoxyethylene sorbitan monooleate

Question 31

Use of Tween 80

Answer 31

Promote growth of lipophilic organisms (Coryneforms and Mycobacterium)

Question 32

Niacin

Answer 32

Nicotinic acid

Question 33

Use of Niacin

Answer 33

Differentiates MTB (Positive) from M. bovis (Negative)

Question 34

T2H/TCH

Answer 34

Thiophene-2-Carboxylic acid Hydrazide

Question 35

Use of T2H/TCH

Answer 35

Differentiates MTB (Resistant) from M. bovis (Sensitive)

Question 36

INH

Answer 36

Isoniconoylhydrazine or Isoniazid

Question 37

Use of INH

Answer 37

One of the primary anti-TB drugs

Question 38

0129 disk

Answer 38

2,4-diamino-6,7-diisopropylpteridine

Question 39

Use of 0129 disk

Answer 39

Differentiate V. cholera O1 and nonO1 (Sensitive) from other Vibrios (Resistant)

Question 40

X factor

Answer 40

Hemin/Protoporphyrin IX

Question 41

V factor

Answer 41

NAD or NADP (Nicotine Adenine Dinucleotide Phosphate)

Question 42

X and V factor

Answer 42

Nutritional growth factors of Haemophilus species

Question 43

Cetrimide

Answer 43

Cethyltrimethyl-ammonium bromide

Question 44

Use of Cetrimide

Answer 44

Isolation of Pseudomonas aeruginosa as other bacteria are inhibited by this detergent

Question 45

DNAse positive (Clearing of agar)

Answer 45

Staphylococcus aureus, Serratia marsescens, Stenotrophomonas maltophilia, Moraxella catarrhalis, Elizabethkingia (Chryseobacterium) meningoseptica

Question 46

0.025 SPS sensitive increased 1% gelatin

Answer 46

Neisseria, Mycoplasma, Gardnerella, Capnocytophaga, Streptobacillus moniliformis, Peptostreptococcus anaerobius

Question 47

Hippurate positive (Purple color)

Answer 47

Streptococcus agalactiae, Listeria monocytogenes, Gardnerella vaginalis, Campylobacter jejuni

Question 48

Intracellular bacteria

Answer 48

Bartonella, Brucella, Yersinia, Mycoplasma, Chlamydia, Rickettsia, Ehrlichia