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BACTERIOLOGY > Indicators of culture media and reagents > Flashcards

Indicators of culture media and reagents Flashcards

1
Q

Gram Positive Aerobic Cocci (SMS)

A

Staphylococcus, Micrococcus, Streptococcus

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2
Q

Gram Positive Anaerobic Cocci

A

Peptostreptococcus, Peptococcus

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3
Q

Gram Positive Aerobic Bacilli

A

Mycobacterium, Corynebacterium, Nocardia, Arcanobacterium, Streptomyces, Rhodococcus, Listeria, Erysipelothrix, Bacillus

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4
Q

Gram Positive Anaerobic Bacilli

A

Clostridium, Cutibacterium, Propionobacterium, Lactobacillus, Actinomyces, Bifidobacterium (dog bone appearance)

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5
Q

Gram Negative Aerobic Cocci

A

Moraxella (Branhamella)

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6
Q

Gram Negative Anaerobic Cocci

A

Veillonella

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7
Q

Gram Negative Capnophile Cocci

A

Neisseria

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8
Q

Gram Negative Aerobic Bacilli

A

Enterobacteria, Pseudomonas, Acidovorax, Burkholderia, Sphingomonas, Vibrio, Aeromonas, Alcaligenes, Francisella, Stenotrophomonas, Acinetobacter, Pasteurella, Bordetella, Brucella, Bartonella

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9
Q

Gram Negative Anaerobic Bacilli (BFPP)

A

Bacteroides, Fusobacterium, Porphyromonas, Prevotella

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10
Q

Gram Negative Microaerophile Bacilli

A

Campylobacter, Helicobacter, Treponema, Borrelia, Leptospira

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11
Q

Indicator for XLD, TSI, KIA, MSA, CTA, viral cell culture

A

Phenol red

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12
Q

Indicator for CIN, MAC, SSA, Ashdown Agar

A

Neutral red

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13
Q

Indicator for citrate agar, HEA, OF tube, TCBS

A

Bromothymol blue

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14
Q

Indicator for LIA, 6.5% NaCl

A

Bromcresol purple

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15
Q

Indicator for DNAse agar

A

Methylene green/Toluidine blue

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16
Q

Indicator for anaerobiosis

A

Resazurin/Methylene blue

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17
Q

Media for H2S detection

A

BSA, KIA, Lead acetate paper strip, SIM, HEA, SSA, LIA, TSI

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18
Q

H2S positive bacteria

A

Erysipelothrix (Gram +), Proteus, Salmonella, Edwardsiella, Brucella abortus, Citrobacter freundii, Shewanella putrefaciens

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19
Q

H2S indicator of BSA and KIA

A

Ferrous sulfate

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20
Q

H2S indicator of Lead acetate paper strip and SIM

A

Lead acetate

21
Q

H2S indicator of HEA, SSA, LIA, TSI

A

Ferric ammonium citrate

22
Q

Taxo A chemical name

A

0.04 U Bacitracin

23
Q

Use of Taxo A

A

Identifying S. pyogenes

24
Q

Taxo P chemical name

A

Ethyhydrocupreine hydrochloride

25
Q

Use of Taxo P

A

Identifying S. pneumoniae

26
Q

NALC name

A

N-acetyl-L-Cysteine A.K.A. Dithiothreitol

27
Q

Use of NALC

A

Digestion reagent for TB sputum samples

28
Q

ZTP

A

Zephiran-Trisodium phosphate

29
Q

Use of ZTP

A

Digestion-Decontamination reagent for TB sputum samples

30
Q

Tween 80

A

Polyoxyethylene sorbitan monooleate

31
Q

Use of Tween 80

A

Promote growth of lipophilic organisms (Coryneforms and Mycobacterium)

32
Q

Niacin

A

Nicotinic acid

33
Q

Use of Niacin

A

Differentiates MTB (Positive) from M. bovis (Negative)

34
Q

T2H/TCH

A

Thiophene-2-Carboxylic acid Hydrazide

35
Q

Use of T2H/TCH

A

Differentiates MTB (Resistant) from M. bovis (Sensitive)

36
Q

INH

A

Isoniconoylhydrazine or Isoniazid

37
Q

Use of INH

A

One of the primary anti-TB drugs

38
Q

0129 disk

A

2,4-diamino-6,7-diisopropylpteridine

39
Q

Use of 0129 disk

A

Differentiate V. cholera O1 and nonO1 (Sensitive) from other Vibrios (Resistant)

40
Q

X factor

A

Hemin/Protoporphyrin IX

41
Q

V factor

A

NAD or NADP (Nicotine Adenine Dinucleotide Phosphate)

42
Q

X and V factor

A

Nutritional growth factors of Haemophilus species

43
Q

Cetrimide

A

Cethyltrimethyl-ammonium bromide

44
Q

Use of Cetrimide

A

Isolation of Pseudomonas aeruginosa as other bacteria are inhibited by this detergent

45
Q

DNAse positive (Clearing of agar)

A

Staphylococcus aureus, Serratia marsescens, Stenotrophomonas maltophilia, Moraxella catarrhalis, Elizabethkingia (Chryseobacterium) meningoseptica

46
Q

0.025 SPS sensitive increased 1% gelatin

A

Neisseria, Mycoplasma, Gardnerella, Capnocytophaga, Streptobacillus moniliformis, Peptostreptococcus anaerobius

47
Q

Hippurate positive (Purple color)

A

Streptococcus agalactiae, Listeria monocytogenes, Gardnerella vaginalis, Campylobacter jejuni

48
Q

Intracellular bacteria

A

Bartonella, Brucella, Yersinia, Mycoplasma, Chlamydia, Rickettsia, Ehrlichia

BACTERIOLOGY (49 decks)

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