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BACTERIOLOGY > Branching Filamentous Gram-Positive > Flashcards

Branching Filamentous Gram-Positive Flashcards

1
Q

Actinomycetes with Mycolic acid in cell wall; Acid-fast

A

Lawsonella, Segniliparus

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2
Q

Actinomycetes with Mycolic acid in cell wall; Partial Acid-fast

A

Nocardia, Rhodococcus, Gordonia, Tsukamurella, some Corynebacterium

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3
Q

Actinomycetes without Mycolic acid in cell wall

A

Nocardiopsis, Actinomadura, Streptomyces, Amycolatopsis, Desmospora, Dermatophilus, Dietzia, Kroppenstedtia, Pseudonocardia, Saccharomonospora, Saccharopolyspora, Thermoactinomyces, Williamsia

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4
Q

Actinomycetes causing pulmonary nocardiosis, mycetoma, catheter-based sepsis, osteomyelitis, skin and UTI infections; affecting old or immunocompromised patients

A

Nocardia spp.

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5
Q

Composition of Nocardia cell wall peptidoglycan

A

Diaminopimelic acid (DAP), Arabinose, Galactose

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6
Q

Appearance of Nocardia in agar

A

Substrate hyphae and aerial hyphae

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7
Q

Microscopic appearance is beaded with hyphae; Partial Acid-fast, Gram stain shows small yellow-orange kidney-shaped with club-like structure

A

Nocardia spp

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8
Q

Nocardia spp. biochemical properties

A

Strictly aerobic, Catalase & Urease positive, Lysozyme resistant

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9
Q

Nocardia culture media

A

SDA, LJ, BHI, BCYE, MTM; Incubate at 30°C for 2-3 weeks at ambient air

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10
Q

Nocardia spp. in tap water agar

A

Extensive hyphae

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11
Q

Distinguishing features of Nocardia asteroides

A

Negative for casein, xanthine, and tyrosine hydrolysis

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12
Q

Distinguishing features of Nocardia brasiliensis

A

Positive for casein and tyrosine hydrolysis

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13
Q

Rhodococcus equi significance

A

Causative agent of pneumonia, UTI, bacteremia, and infections in immunocompromised patients (HIV)

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14
Q

Appearance of Rhodococcus equi colonies

A

Mucoid orange to red or salmon-pink non-hemolytic colonies

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15
Q

Microscopic appearance is gram-positive coccobacilli with zigzag scanty branching pattern

A

Rhodococcus equi

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16
Q

Biochemical properties of Rhodococcus equi

A

Positive for Urease, Catalase, and CAMP

17
Q

Causative agent for pericarditis, bacteremia, brain abscess, actinomycetoma with glabrous waxy heaped gray-white colonies under the microscope

A

Streptomyces spp.

18
Q

Microscopic appearance of a gram-positive atypical bacilli with spore-like bodies and extensive branching; musty basement odor; lysozyme sensitive

A

Streptomyces spp.

19
Q

Streptomyces spp. growth characteristics

A

Extensive hyphae in tap water agar

20
Q

Causative agent for Whipple’s disease (GIT infection with impaired absorption and digestion)

A

Tropheryma whipplei

21
Q

Tropheryma whipplei characteristics

A

Gram-positive actinomycete

22
Q

Gold standard for Tropheryma whipplei diagnosis

A

PAS stain in gastrointestinal biopsy

23
Q

First identification test of Tropheryma whipplei

A

PCR of duodenum biopsy

BACTERIOLOGY (49 decks)

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