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BACTERIOLOGY > BACTERIAL PHYSIOLOGY AND GROWTH REQ > Flashcards

BACTERIAL PHYSIOLOGY AND GROWTH REQ Flashcards

1
Q

Obligate aerobe requirements

A

21% O2, 0.03% CO2

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2
Q

Usual incubator for obligate aerobe

A

Ambient air/Room air

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3
Q

Microorganisms in obligate aerobe

A

Fungi, Mycobacterium tuberculosis (MTB), Brucella, Bordetella

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4
Q

Obligate anaerobe requirements

A

0% O2, 5-10% H2, 5-10% CO2, 80-90% N2

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5
Q

Usual incubator for obligate anaerobe

A

Gas Pak Jar, AnaeroPack, Palladium, Brewer jar, McIntosh & Fildes

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6
Q

Microorganisms in obligate anaerobe

A

Clostridium, Bacteroides

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7
Q

Facultative anaerobe requirements

A

Do not require O2 but grow better with O2

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8
Q

Usual incubator for facultative anaerobe

A

Ambient air/Room air

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9
Q

Microorganisms in facultative anaerobe

A

Enterobacteria (Most pathogens)

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10
Q

Aerotolerant anaerobe requirements

A

Grow at the same rate with or without O2

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11
Q

Usual incubator for aerotolerant anaerobe

A

Ambient air/Room air

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12
Q

Microorganism in aerotolerant anaerobe

A

Cutibacterium (Propionibacterium) acnes

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13
Q

Bacteria’s dry weight

A

50% Carbon and 14% Nitrogen

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14
Q

Use Carbon dioxide as sole source of carbon

A

Autotrophs

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15
Q

Use light as source of energy

A

Phototrophs

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16
Q

Use inorganic compounds as source of energy

A

Chemolithotrophs

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17
Q

Require complex substances for growth; require organic substances for energy; clinically significant bacteria causing human infection

A

Heterotrophs

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18
Q

Psychrophiles/Cryophiles (0-20ºC); Refrigerator contamination

A

Listeria, Y. enterocolitica

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19
Q

Psychrophiles/Cryophiles (0-20ºC): Blood bank contamination: Y. enterocolitica, P. fluorescens, S. liquifaciens

A
20
Q

Mesophiles (20-40ºC)

A

Room temp contamination; Blood bank: S. epidermidis, B. cereus

21
Q

Thermophiles (40-60ºC)

A

Bacillus stearothermophilus - autoclave

22
Q

Room temperature (20-25ºC)

A

Best for microbial pigment production; use sterile white swab to pick up colony

23
Q

Incubator temperature

A

35-37ºC

24
Q

Fungal cultures

A

30ºC

25
Q

Cold enrichment (4ºC)

A

Listeria, Y. enterocolitica

26
Q

Hot enrichment (42ºC)

A

Campylobacter

27
Q

Acidophilic (<5.5 pH)

A

Lactobacillus acidophilus (Doderlein bacilli in vagina, Boas Oppler bacilli in GI tract)

28
Q

Alkalinophilic (>8.0 pH)

A

Vibrio spp.

29
Q

Bacterial vaginosis (alkaline pH)

A

Gardnerella vaginalis, Mobiluncus spp. (Gram-variable, curved anaerobic bacilli)

30
Q

Salt-loving bacteria; require NaCl for growth

A

Halophiles

31
Q

S. aureus salt tolerance amount

A

7.5% NaCl (MSA)

32
Q

Enterococcus salt tolerance amount

A

6.5% NaCl broth

33
Q

Vibrio spp amount of salt tolerance.

A

0, 3, 5, 8, 10% NaCl broth (All Vibrio are halophilic except cholera and mimicus)

34
Q

Microaerophile requirements

A

5-10% O2 (decreased), 8-10% CO2 (increased), 85% N2

35
Q

Usual incubator for microaerophile

A

Microaerophilic incubator or Jars

36
Q

Microorganisms in microaerophile

A

Campylobacter, Helicobacter, Treponema, Borrelia

37
Q

Capnophile requirements

A

5-10% CO2 (increased)

38
Q

Usual incubator for capnophile

A

Candle Jar, Capnophilic incubator

39
Q

Microorganisms in capnophile

A

Haemophilus, HACEK, Neisseria, Streptococcus pneumoniae

40
Q

Indicators of anaerobiosis

A

Methylene Blue and Resazurin (With color = With Oxygen; Colorless = Anaerobiosis)

41
Q

Special growth supplement for Haemophilus

A

X and V factors, IsoVitale X or Vitox

42
Q

Special growth supplement for Legionella

A

Cysteine and Iron

43
Q

Special growth supplement for Francisella

A

Cysteine and Cystine

44
Q

Special growth supplement for Bordetella pertussis

A

Cysteine, Methionine, Nicotinic acid

45
Q

Special growth supplement for Granulicatella and Abiotrophia

A

Cysteine and Vitamin B6 (Pyridoxal)

46
Q

Special growth supplement for Anaerobes (Bacteroides)

A

Hemin and Vitamin K

47
Q

Special growth supplement for B. anthracis and Trichophyton fungus

A

Vitamin B1 (thiamine)

BACTERIOLOGY (49 decks)

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