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BACTERIOLOGY > BACTERIAL GROWTH CURVE AND BACTERIA > Flashcards

BACTERIAL GROWTH CURVE AND BACTERIA Flashcards

1
Q

Generation time can be every

A

30 minutes (E. coli) to every 24 hours (M. tuberculosis)

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2
Q

Increase in cell size, preparation to multiply

A

Adjustment/Lag phase

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3
Q

Increase in number of living cells; sensitive to antimicrobials

A

Exponential/Log phase

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4
Q

Equal number of dead and alive cells (constant number of cells); Decrease in nutrients; Increase in toxins; Sporulation occurs (Bacillus, Clostridium)

A

Stationary/Plateau phase

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5
Q

Increase in number of dead cells; more dead cells than alive cells

A

Death/Decline phase

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6
Q

In general Culture media preparation contains

A

pH: 7.2-7.4, distilled/deionized water; Dissolved till clear and no particles seen

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7
Q

Sterilization method for general culture media

A

AUTOCLAVE 121°C, 15PSI, 15 minutes

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8
Q

Sterilization method for heat-labile or delicate media

A

116-118°C or membrane filtration (pore size 0.2-0.45μm)

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9
Q

Types of Bacterial Culture

A

Pure culture, Mixed culture, Stock culture

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10
Q

Most common solidifying agent in bacterial culture

A

Agarose

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11
Q

Step after media sterilization and cooling

A

Add supplements (blood, vitamins, nutrients, or antibiotics)

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12
Q

Volume of cooled media dispensed on a 100 mm petri dish

A

20-25 mL at 55-60°C

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13
Q

LIQUID media examples

A

BHIB, THIO broth, TSB

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14
Q

SEMI-SOLID media example

A

SIM (0.5-1% agar)

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15
Q

SOLID media examples

A

TSI, MAC, CHOC (2-3% agar)

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16
Q

SYNTHETIC/DEFINED media

A

For research, all content is known

17
Q

NONSYNTHETIC/COMPLEX media

A

Has some unknown content; examples: Nutrient broth, MAC

18
Q

TISSUE CULTURE media for obligate intracellular organisms

A

Chlamydia, Rickettsia - McCoy, Vero, HeLa, Hep2

19
Q

Sterilize and Dispense using petri dish

A

PLATED MEDIA

20
Q

Dispense and Sterilize, examples: Agar Deep, Agar Slant, Butt-Slant media

A

TUBED MEDIA

21
Q

Routinely used without special supplements for non-fastidious organisms (e.g., Nutrient agar, Trypticase soy)

A

SIMPLE/GENERAL PURPOSE/NUTRITIVE media

22
Q

Grow certain groups with supplements while suppressing other flora (e.g., APW, Selenite F, BCYE, THIO, LIM)

A

ENRICHMENT MEDIA

23
Q

For fastidious organisms (e.g., BAP, CAP)

A

ENRICHED MEDIA

24
Q

Visualizes metabolic differences of bacteria (e.g., MAC, BAP, EMB, HEA)

A

DIFFERENTIAL MEDIA

25
Q

Contains inhibitors to grow specific groups of bacteria (e.g., HEA, MAC, XLD, MSA)

A

SELECTIVE MEDIA

26
Q

Isolate bacteria with specific requirements (e.g., LJ, TCBS, Cysteine agar)

A

SPECIAL MEDIA

27
Q

Inhibitors that inhibit Gram-positive bacteria

A

Crystal violet, basic fuchsin, bile salt, sodium deoxycholate

28
Q

Inhibitors that inhibit Gram-negative bacteria

A

Phenylethyl alcohol, potassium tellurite, sodium azide

29
Q

Inhibitors for swarming Proteus spp

A

Alcohol, chloral hydrate

30
Q

Base agar for Neisseria

A

Chocolate agar

31
Q

Thayer Martin agar components

A

VCN (Vancomycin, Colistin, Nystatin)

32
Q

Modified Thayer-Martin agar components

A

VCNT (Vancomycin, Colistin, Nystatin, Trimethoprim)

33
Q

NYC agar components

A

VCAT (Amphotericin B)

34
Q

Martin-Lewis agar components

A

VCAT (Amphotericin B, Anisomycin)

35
Q

GC agar components

A

VCATL (Amphotericin B)

36
Q

NYC agar supports growth of

A

Neisseria, Ureaplasma, some Mycoplasma

37
Q

Antimicrobials for Neisseria culture media

A

Vancomycin, Lincomycin, Colistin, Nystatin, Amphotericin B, Anisomycin

38
Q

Antimicrobials for Neisseria growth inhibition

A

Vancomycin (Gram positive), Colistin (Gram negative), Nystatin, Amphotericin B (Fungi)

BACTERIOLOGY (49 decks)

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