ESKAPE GROUP and AACEK (HACEK) Flashcards

1
Q

Group of highly virulent, antibiotic-resistant, nosocomial infection-associated bacteria

ESKAPE group

A

Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp.

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2
Q

Characteristic of Haemophilus spp.

A

Small, nonmotile, capnophilic, pleomorphic Gram-negative bacilli or coccobacilli, requiring X (hemin) and V (NAD) factors

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3
Q

Capsule composition of Haemophilus influenzae

A

Sugar-alcohol (polyribitol) phosphate, 6 capsular serotypes: a to f

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4
Q

Infections caused by Haemophilus influenzae

A

Meningitis, Epiglottitis, Cellulitis, Arthritis, Pneumonia, Otitis Media

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5
Q

Transmission route of Haemophilus influenzae

A

Respiratory droplets

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6
Q

Diagnostic test for Haemophilus influenzae in CSF and urine

A

Particle agglutination

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7
Q

Condition caused by Haemophilus influenzae biotype aegypticus

A

Brazilian purpuric fever

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8
Q

Common infections caused by Haemophilus parainfluenzae

A

Acute otitis media, sinusitis, bacteremia, culture negative endocarditis

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9
Q

Common infection caused by Haemophilus aegypticus

A

Purulent conjunctivitis (pink eye)

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10
Q

Condition caused by Haemophilus ducreyi

A

Chancroid/Soft chancre (painful genital ulcers), an STD

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11
Q

Microscopic appearance of Haemophilus ducreyi

A

School of red fish (red under microscope, Gram-negative)

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12
Q

Haemophilus spp. factor requirements:
X and V

X (hemin) factor check, porphyrin absent;
V factor = NAD; Needs both X and V, with absent porphyrin

A

1st row:

H. influenzae,
H. aegypticus,
H. haemolyticus

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13
Q

Haemophilus spp. factor requirements: X factor absent and V factor needed

absent X factor and needs V factor
positive for porphyrin

A

2nd row:

H. parainfluenzae,
H.parahaemolyticus,
H. paraphrophilus

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14
Q

Haemophilus spp. factor requirements: X factor needed and V factor absent;

Needs X factor and absent V factor, absent porphyrin

A

3rd row: H. ducreyi

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15
Q

Haemophilus spp. with no factor requirements and positive for porphyrin.

Doesn’t need X and V factors. Present porphyrin

A

Aggregatibacter aphrophilus

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16
Q

Media for X and V factor test

A

Media: Heart infusion agar

17
Q

Reagent: Disk/strip for X and V factor and incubation time

A

Impregnated with X, V and XV factors
Incubate overnight
35 deg C
ambient air

18
Q

Positive test result for Haemophilus spp. with X and V factor:

Growth around XV disk only

A

1st row organisms (IHA - XV disk only)

H. influenzae,
H. aegypticus,
H. haemolyticus

19
Q

Positive test result for Haemophilus spp. with X and V factor:

Growth around XV and V disks only

A

2nd row organisms PPP - XV and V growth

H. parainfluenzae,
H.parahaemolyticus,
H. paraphrophilus

20
Q

Positive test result for Haemophilus spp. with X and V factor:

Growth around XV and X disks only

A

3rd row organism: XV, X - CRY

H. ducreyi

21
Q

Negative test result for Haemophilus spp. with X and V factor:

Growth around XV, X, and V disks

A

Non-fastidious organisms

22
Q

Dysgonic small gram-negative (cocco)bacilli

Normal oral flora; occasionally associated with Subacute Bacterial Endocarditis

Recovered in blood culture then Subculture in BAP/CAP (Candle jar incubation)

A

AACEK (HACEK)

Aggregatibacter (Haemophilus) aphrophilus
Aggregatibacter (Actinobacillus) actinomycetemcomitans
Cardiobacterium hominis
Eikenella corrodens
Kingella kingae

23
Q

AACEK that causes Dental plaques

A

Aggregatibacter (Haemophilus) aphrophilus

24
Q

AACEK that causes Periodontitis; Coinfection with Actinomyces

A

Aggregatibacter (Actinobacillus) actinomycetemcomitans

25
AACEK that is frequently associated with **aortic valve infection**
Cardiobacterium hominis
26
AACEK associated with **Human bite infection**
Eikenella corrodens
27
AACEK associated with **Joint and bone infections; osteoarthritis in children**
Kingella kingae
28
X factor dependent, Nitrate reduction positive
Aggregatibacter (Haemophilus) aphrophilus
29
**Star-shaped centers in BHIA (dots and dashes of Morse code)**
Aggregatibacter (Actinobacillus) actinomycetemcomitans
30
**Only Indole positive in AACEK** False gram-positive staining **PITS Rosette (rose petal)** formation with stick-like projections
Cardiobacterium hominis
31
**Bleach-like odor** Pits agar, umbonate colony
Eikenella corrodens
32
Narrow zone of beta-hemolysis in BAP
Kingella kingae