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BACTERIOLOGY > Curved, Oxidase positive, Gram-Negative > Flashcards

Curved, Oxidase positive, Gram-Negative Flashcards

1
Q

Curved, Oxidase-positive, Gram-negative Bacilli General

Genus Included

A

Vibrio, Campylobacter, Arcobacter, Helicobacter

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2
Q

General Features

A

Catalase Positive, Oxidase Positive, Agents of Gastroenteritis

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3
Q

Vibrio spp. Characteristics

A

Gram-negative, Curved/Comma-shaped Bacilli, Catalase Positive, Oxidase Positive, Indole Positive, Glucose Positive, Motile via Polar Flagella, Facultative Anaerobe, Found in Salt/Brackish/Marine Water

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4
Q

Vibrio spp. Halophilic Exception

A

Vibrio cholerae and Vibrio mimicus are not halophilic

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5
Q

Vibrio spp. Infections

A

Cholera, Septicemia, Wound Infections

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6
Q

Cholera Pathophysiology

A

Over secretion of cAMP leading to profuse watery diarrhea, dehydration, and death

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7
Q

Vibrio cholerae Virulence Factors

A

Choleragen (enterotoxin for cholera), Cholera toxin (CT), Zonula occludens toxin (Zot), Accessory cholera toxin (Ace), O1 and O139 somatic antigens, Hemolysin, Cytotoxin, Flagella, Mucinase, Toxin Coregulated Pili (TCP)

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8
Q

Pandemic Strain of Vibrio cholerae

A

Pandemic strain: VC-O1, Biotype: El Tor

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9
Q

Serotypes of Vibrio cholerae

A

Ogawa, Inaba, Hikojima

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10
Q

Specimen Hallmark of Vibrio cholerae

A

Rice watery stool

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11
Q

Transport Media for Vibrio cholerae

A

Cary Blair

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12
Q

Enrichment Media for Vibrio cholerae

A

Alkaline Peptone Water (pH 8.4)

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13
Q

Cultural Media for Vibrio cholerae

A

Thiosulfate-citrate-bile salts-sucrose (TCBS) (pH 8.6)

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14
Q

Tests for Vibrio cholerae

A

String test positive, Vibriostatic test (0129-impregnated disk) sensitive

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15
Q

General Genus of Curved, Oxidase Positive, Gram-Negative Bacilli

A

Vibrio, Campylobacter, Arcobacter, Helicobacter

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16
Q

Gram Negative Curved/Comma-Shaped Bacilli, Catalase, Oxidase, Indole, Glucose Positive, Motile via Polar Flagella, Facultative Anaerobe, Found in Salt/Brackish/Marine Water

A

Vibrio spp.

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17
Q

All Vibrio spp are halophilic except

A

V. cholerae and V. mimicus

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18
Q

Infections of Vibrio spp.

A

Cholera, Septicemia, Wound Infections

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19
Q

Cholera Pathophysiology

A

Over secretion of cAMP causing profuse watery diarrhea leading to dehydration and death

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20
Q

Associated Condition with Vibrio cholerae

A

Thrombotic thrombocytopenic purpura

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21
Q

Virulence Factors of Vibrio cholerae

A

Choleragen (enterotoxin), Cholera toxin (CT), Zonula occludens toxin (Zot), Accessory cholera toxin (Ace), O1 and O139 Somatic antigens, Hemolysin, Cytotoxin, Flagella, Mucinase, Toxin Coregulated Pili (TCP)

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22
Q

Pandemic Strain of Vibrio cholerae

A

Pandemic strain: VC-O1, Biotypes: El Tor

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23
Q

Serotypes of Vibrio cholerae

A

Ogawa, Inaba, Hikojima

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24
Q

Specimen Hallmark of Vibrio cholerae

A

Rice watery stool

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25
Q

Transport Media for Vibrio cholerae

A

Cary Blair

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26
Q

Enrichment Media for Vibrio cholerae

A

Alkaline Peptone Water (pH 8.4)

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27
Q

Cultural Media for Vibrio cholerae

A

TCBS (pH 8.6)

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28
Q

Diagnostic Tests for Vibrio cholerae

A

String test positive, Vibriostatic test (0129-impregnated disk) sensitive

29
Q

Sucrose Fermenter; Yellow in TCBS

A

V. cholerae, V. alginolyticus, V. furnissii, V. fluvialis, V. cincinnatiensis, V. metschnikovii

30
Q

Non-Sucrose Fermenter; Green in TCBS

A

V. parahemolyticus, V. vulnificus, V. mimicus

31
Q

Cholera; Rice water stool

A

V. cholerae

32
Q

Otitis, wound infection, gastroenteritis

A

V. alginolyticus

33
Q

Summer diarrhea, B-hemolysis in Wagatsuma agar, Kanagawa phenomenon

A

V. parahemolyticus

34
Q

Septicemia, wound infection, Lactose +, (ONPG +)

A

V. vulnificus

35
Q

Seen in Aquatic environments: Freshwater, polluted, chlorinated, brackish water, occasionally marine water

A

Aeromonas spp.

36
Q

Causes Human: Gastroenteritis, wound infection, bacteremia; Reptile: Red-leg syndrome

A

Aeromonas caviae complex, Aeromonas hydrophila complex

37
Q

Red man syndrome due to in reptiles

A

Vancomycin

38
Q

Gram negative straight bacilli with rounded ends, Oxidase positive, Bull’s eye colonies on CIN, Beta-hemolytic on BAP, Do not grow on TCBS, Not halophilic, grows with 0% NaCl

A

Aeromonas spp.

39
Q

Gram-negative Curved/Seagull wing-shaped bacilli, Catalase, Oxidase, Indoxyl acetate Positive

A

Campylobacter spp.

40
Q

Campylobacter spp. are microaerophilic and grow at

A

42°C (hot enrichment method)

41
Q

Associated with gastroenteritis, reactive arthritis (Reiter syndrome), and Guillain-Barré syndrome

A

Campylobacter spp.

42
Q

Improved staining for Campylobacter spp.

A

0.1% basic fuchsin

43
Q

Culture media for Campylobacter spp.

A

Skirrow, CAMPY-CVA, Cefoperazone agar, Charcoal-based selective medium (CSM)

44
Q

Growth at 32°C and 42°C, Nalidixic acid sensitive, Cephalotin resistant; Hippurate +

A

C. jejuni

45
Q

Growth at 32°C and 42°C, Nalidixic acid sensitive, Cephalotin resistant; Hippurate -

A

C. coli

46
Q

Source of C. jejuni

A

Poultry, Pig, Bull, Dog, Cat

47
Q

Source of C. coli

A

Poultry, Pig, Bull, Sheep

48
Q

Growth at 37°C, Nalidixic acid resistant, Cephalotin sensitive, Found in cattle and sheep

A

C. fetus

49
Q

Causes gastroenteritis and septicemia

A

“Arcobacter spp.”

50
Q

Method to enhance recovery of Campylobacter and Arcobacter in stool samples using 0.45 to 0.65 µm pore size filters

A

“Filtration method”

51
Q

Curved Gram-negative bacilli, Oxidase positive, weak Catalase positive, Urease negative

A

“Arcobacter spp.”

52
Q

Causes Peptic Ulcer and Peptic Cancer

A

“Helicobacter pylori”

53
Q

Virulence factor of H. pylori that produces exotoxin creating vacuoles and loosens tight junctions

A

“VacA”

54
Q

Virulence factor of H. pylori that is part of pathogenicity islands causing peptic carcinoma

A

“CagA”

55
Q

Virulence factor of H. pylori that binds Leb antigens in gastric epithelial cells

A

“BabA”

56
Q

Virulence factor of H. pylori associated with peptic ulcers

A

“IceA”

57
Q

Causes gastroenteritis and septicemia

A

“Arcobacter spp.”

58
Q

Method to enhance recovery of Campylobacter and Arcobacter in stool samples using 0.45 to 0.65 µm pore size filters

A

“Filtration method”

59
Q

Curved Gram-negative bacilli, Oxidase positive, weak Catalase positive, Urease negative

A

“Arcobacter spp.”

60
Q

Causes Peptic Ulcer and Peptic Cancer

A

“Helicobacter pylori”

61
Q

Virulence factor of H. pylori that produces exotoxin creating vacuoles and loosens tight junctions

A

“VacA”

62
Q

Virulence factor of H. pylori that is part of pathogenicity islands causing peptic carcinoma

A

“CagA”

63
Q

Virulence factor of H. pylori that binds Leb antigens in gastric epithelial cells

A

“BabA”

64
Q

Virulence factor of H. pylori associated with peptic ulcers

A

“IceA”

65
Q

Other Helicobacter species isolated from bacteremic homosexual men with HIV

A

“H. rappini, H. cinaedi, H. fennelliae”

66
Q

Tests positive for Catalase, Oxidase, Motility, Urease (Urea Breath Test)

A

“Helicobacter spp.”

67
Q

Growth requirements for Helicobacter spp.

A

“Microaerophilic, Curved Gram-negative bacilli”

68
Q

Transport media for tissue biopsy of H. pylori

A

“Brucella broth with glycerol, Stuart’s transport medium, Portagerm pylori media”

69
Q

Stains used for H. pylori

A

0.1% basic Fuchsin, Warthin-Starry (silver), Giemsa

BACTERIOLOGY (49 decks)

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