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BACTERIOLOGY > Curved, Oxidase positive, Gram-Negative > Flashcards

Curved, Oxidase positive, Gram-Negative Flashcards

1
Q

Curved, Oxidase-positive, Gram-negative Bacilli General

Genus Included

A

Vibrio, Campylobacter, Arcobacter, Helicobacter

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2
Q

General Features: Curved, Oxidase-positive, Gram-negative Bacilli General

A

Catalase Positive, Oxidase Positive, Agents of Gastroenteritis

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3
Q

Vibrio spp. Characteristics

A

Gram-negative, Curved/Comma-shaped Bacilli, Catalase Positive, Oxidase Positive, Indole Positive, Glucose Positive, Motile via Polar Flagella, Facultative Anaerobe, Found in Salt/Brackish/Marine Water

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4
Q

Vibrio spp. Halophilic Exception

A

Vibrio cholerae and Vibrio mimicus are not halophilic

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5
Q

Vibrio spp. Infections

A

Cholera, Septicemia, Wound Infections

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6
Q

Cholera Pathophysiology

A

Over secretion of cAMP leading to profuse watery diarrhea, dehydration, and death

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7
Q

Vibrio cholerae Virulence Factors

A

Choleragen (enterotoxin for cholera), Cholera toxin (CT), Zonula occludens toxin (Zot), Accessory cholera toxin (Ace), O1 and O139 somatic antigens, Hemolysin, Cytotoxin, Flagella, Mucinase, Toxin Coregulated Pili (TCP)

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8
Q

Pandemic Strain of Vibrio cholerae

A

Pandemic strain: VC-O1, Biotype: El Tor

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9
Q

Serotypes of Vibrio cholerae

A

Ogawa, Inaba, Hikojima

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10
Q

Specimen Hallmark of Vibrio cholerae

A

Rice watery stool

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11
Q

Transport Media for Vibrio cholerae

A

Cary Blair

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12
Q

Enrichment Media for Vibrio cholerae

A

Alkaline Peptone Water (pH 8.4)

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13
Q

Cultural Media for Vibrio cholerae

A

Thiosulfate-citrate-bile salts-sucrose (TCBS) (pH 8.6)

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14
Q

Tests for Vibrio cholerae

A

String test positive, Vibriostatic test (0129-impregnated disk) sensitive

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15
Q

General Genus of Curved, Oxidase Positive, Gram-Negative Bacilli

A

Vibrio, Campylobacter, Arcobacter, Helicobacter

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16
Q

Gram Negative Curved/Comma-Shaped Bacilli, Catalase, Oxidase, Indole, Glucose Positive, Motile via Polar Flagella, Facultative Anaerobe, Found in Salt/Brackish/Marine Water

A

Vibrio spp.

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17
Q

All Vibrio spp are halophilic except

A

V. cholerae and V. mimicus

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18
Q

Infections of Vibrio spp.

A

Cholera, Septicemia, Wound Infections

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19
Q

Cholera Pathophysiology

A

Over secretion of cAMP causing profuse watery diarrhea leading to dehydration and death

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20
Q

Associated Condition with Vibrio cholerae

A

Thrombotic thrombocytopenic purpura

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21
Q

Virulence Factors of Vibrio cholerae

A

Choleragen (enterotoxin), Cholera toxin (CT), Zonula occludens toxin (Zot), Accessory cholera toxin (Ace), O1 and O139 Somatic antigens, Hemolysin, Cytotoxin, Flagella, Mucinase, Toxin Coregulated Pili (TCP)

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22
Q

Pandemic Strain of Vibrio cholerae

A

Pandemic strain: VC-O1, Biotypes: El Tor

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23
Q

Serotypes of Vibrio cholerae

A

Ogawa, Inaba, Hikojima

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24
Q

Specimen Hallmark of Vibrio cholerae

A

Rice watery stool

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25
Transport Media for Vibrio cholerae
Cary Blair
26
Enrichment Media for Vibrio cholerae
Alkaline Peptone Water (pH 8.4)
27
Cultural Media for Vibrio cholerae
TCBS (pH 8.6)
28
Diagnostic Tests for Vibrio cholerae
String test positive, Vibriostatic test (0129-impregnated disk) sensitive
29
Sucrose Fermenter; Yellow in TCBS
V. cholerae, V. alginolyticus, V. furnissii, V. fluvialis, V. cincinnatiensis, V. metschnikovii
30
Non-Sucrose Fermenter; Green in TCBS
V. parahemolyticus, V. vulnificus, V. mimicus
31
Cholera; Rice water stool
V. cholerae
32
Otitis, wound infection, gastroenteritis
V. alginolyticus
33
Summer diarrhea, B-hemolysis in Wagatsuma agar, Kanagawa phenomenon
V. parahemolyticus
34
Septicemia, wound infection, Lactose +, (ONPG +)
V. vulnificus
35
Seen in Aquatic environments: Freshwater, polluted, chlorinated, brackish water, occasionally marine water
Aeromonas spp.
36
Causes Human: Gastroenteritis, wound infection, bacteremia; Reptile: Red-leg syndrome
Aeromonas caviae complex, Aeromonas hydrophila complex
37
Red man syndrome due to in reptiles
Vancomycin
38
Gram negative straight bacilli with rounded ends, Oxidase positive, Bull’s eye colonies on CIN, Beta-hemolytic on BAP, Do not grow on TCBS, Not halophilic, grows with 0% NaCl
Aeromonas spp.
39
Gram-negative Curved/Seagull wing-shaped bacilli, Catalase, Oxidase, Indoxyl acetate Positive
Campylobacter spp.
40
Campylobacter spp. are microaerophilic and grow at
42°C (hot enrichment method)
41
Associated with gastroenteritis, reactive arthritis (Reiter syndrome), and Guillain-Barré syndrome
Campylobacter spp.
42
Improved staining for Campylobacter spp.
0.1% basic fuchsin
43
Culture media for Campylobacter spp.
Skirrow, CAMPY-CVA, Cefoperazone agar, Charcoal-based selective medium (CSM)
44
Growth at 32°C and 42°C, Nalidixic acid sensitive, Cephalotin resistant; Hippurate +
C. jejuni
45
Growth at 32°C and 42°C, Nalidixic acid sensitive, Cephalotin resistant; Hippurate -
C. coli
46
Source of C. jejuni
Poultry, Pig, Bull, Dog, Cat
47
Source of C. coli
Poultry, Pig, Bull, Sheep
48
Growth at 37°C, Nalidixic acid resistant, Cephalotin sensitive, Found in cattle and sheep
C. fetus
49
Causes gastroenteritis and septicemia
"Arcobacter spp."
50
Method to enhance recovery of Campylobacter and Arcobacter in stool samples using 0.45 to 0.65 µm pore size filters
"Filtration method"
51
Curved Gram-negative bacilli, Oxidase positive, weak Catalase positive, Urease negative
"Arcobacter spp."
52
Causes Peptic Ulcer and Peptic Cancer
"Helicobacter pylori"
53
Virulence factor of H. pylori that produces exotoxin creating vacuoles and loosens tight junctions
"VacA"
54
Virulence factor of H. pylori that is part of pathogenicity islands causing peptic carcinoma
"CagA"
55
Virulence factor of H. pylori that binds Leb antigens in gastric epithelial cells
"BabA"
56
Virulence factor of H. pylori associated with peptic ulcers
"IceA"
57
Causes gastroenteritis and septicemia
"Arcobacter spp."
58
Method to enhance recovery of Campylobacter and Arcobacter in stool samples using 0.45 to 0.65 µm pore size filters
"Filtration method"
59
Curved Gram-negative bacilli, Oxidase positive, weak Catalase positive, Urease negative
"Arcobacter spp."
60
Causes Peptic Ulcer and Peptic Cancer
"Helicobacter pylori"
61
Virulence factor of H. pylori that produces exotoxin creating vacuoles and loosens tight junctions
"VacA"
62
Virulence factor of H. pylori that is part of pathogenicity islands causing peptic carcinoma
"CagA"
63
Virulence factor of H. pylori that binds Leb antigens in gastric epithelial cells
"BabA"
64
Virulence factor of H. pylori associated with peptic ulcers
"IceA"
65
Other *Helicobacter* species isolated from bacteremic homosexual men with HIV
"H. rappini, H. cinaedi, H. fennelliae"
66
Tests positive for Catalase, Oxidase, Motility, Urease (Urea Breath Test)
"Helicobacter spp."
67
Growth requirements for *Helicobacter spp.*
"Microaerophilic, Curved Gram-negative bacilli"
68
Transport media for tissue biopsy of *H. pylori*
"Brucella broth with glycerol, Stuart’s transport medium, Portagerm pylori media"
69
Stains used for *H. pylori*
0.1% basic Fuchsin, Warthin-Starry (silver), Giemsa

BACTERIOLOGY (49 decks)

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