Bacteriologic Reactions of Milk Flashcards
Fermentation, Gas-production
Clostridium perfringens, Lactobacillus acidophilus
Stormy fermentation
Clostridium perfringens, Clostridium butyricum
Souring of milk
Streptococcus lactis
Clot formation
Lactobacillus acidophilus
Gas-forming, Casein hydrolysis
Coliform (enterobacteria)
Proteolysis on coagulated milk
Bacillus subtilis
Blue milk
Pseudomonas syncyanea
Blue-green milk, Peptonization (clearing)
Pseudomonas aeruginosa
Reduction and Peptonization of milk
Pseudomonas aeruginosa
Red milk
Serratia marcescens
Yellow milk
Flavobacterium synxanthum
Slimy/Ropy milk
Alcaligenes viscosus
Litmus milk test, Fermentation, Gas production
Clostridium perfringens
Litmus milk test, Clot formation, Acid production (Pink/Mauve color)
Lactobacillus acidophilus
Litmus milk test, Clearing, Peptonization (Watery fluid and shrunken insoluble blue clot)
Pseudomonas aeruginosa
Lactobacillus MRS Broth, (+) Growth, (+) Gas
Leuconostoc
Lactobacillus MRS Broth, (+) Growth, (-) Gas
Lactobacillus
Lactobacillus MRS Broth, Negative
Escherichia coli
Aids in ID of some GP and GN bacilli
Litmus milk test
Detects gas formers from glucose fermentation
Lactobacillus MRS broth
Principle of Litmus milk test
Lactose fermentation → acid, Acid + casein → clot
Principle of Lactobacillus MRS broth
Inhibitor of contaminating organisms: Na acetate & Ammonium citrate
Medium for Litmus milk test
Litmus milk medium
Medium for Lactobacillus MRS broth
MRS broth with vitamins
Indicator for Litmus milk test
Litmus
Indicator for Lactobacillus MRS broth
Durham tube
Incubation for Lactobacillus MRS broth
35-37°C, 24-48 hours
Incubation for Litmus milk test
35-37°C, Observe daily up to 7 days
